Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,745,602	T→C	Y106C (TAC→TGC)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,602	0	T	C	100.0%	156.7 / NA	44	Y106C (TAC→TGC)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

CGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAG  >  NC_000913/2745475‑2745733                                                                                                                                |                                                                                                                                    cGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCtt                                                                                                                         <  1:210599/140‑1 (MQ=255)  gCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTc                                                                                                                        >  2:761438/1‑140 (MQ=255)     tCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAg                                                                                                                     <  1:10883/140‑1 (MQ=255)       tttCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTccc                                                                                                                               >  1:252797/1‑128 (MQ=255)       tttCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTccc                                                                                                                               <  2:252797/128‑1 (MQ=255)         tCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGa                                                                                                                 >  2:525578/1‑140 (MQ=255)           aGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTccc                                                                                                                               >  2:627958/1‑124 (MQ=255)           aGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTccc                                                                                                                               <  1:627958/124‑1 (MQ=255)           aGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAgg                                                                                                               <  1:412219/140‑1 (MQ=255)                  ccTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGc                                                                                                        >  2:410808/1‑140 (MQ=255)                             agaACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGAt                                                                                                     <  2:825379/132‑1 (MQ=255)                             agaACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGAt                                                                                                     >  1:825379/1‑132 (MQ=255)                                                    catcatATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTcagca                                                                      >  2:390583/1‑140 (MQ=255)                                                     atcatATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTTTTCAAGCTGAGGCAGCTGCGATGAATCCACGGCAATTGCACAATTCGTcagcag                                                                     <  1:877492/140‑1 (MQ=255)                                                          atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTcag                                                                        <  1:14670/132‑1 (MQ=255)                                                          atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTcag                                                                        >  2:14670/1‑132 (MQ=255)                                                          atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTcg                                                                >  1:240039/1‑140 (MQ=255)                                                           tCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTcgc                                                               <  1:926020/140‑1 (MQ=255)                                                              acgacTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTcgc                                                               >  1:32558/1‑137 (MQ=255)                                                              acgacTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTcgc                                                               <  2:32558/137‑1 (MQ=255)                                                               cgacTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCa                                                           <  1:410808/140‑1 (MQ=255)                                                                     tACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGa                                                     >  2:281470/1‑140 (MQ=255)                                                                                      tCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGt                                                 <  1:417234/127‑1 (MQ=255)                                                                                      tCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGt                                                 >  2:417234/1‑127 (MQ=255)                                                                                       cAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGc                                   >  2:715445/1‑140 (MQ=255)                                                                                        aGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCt                                  >  1:222458/1‑140 (MQ=255)                                                                                               actacCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTgatgat                           <  1:281470/140‑1 (MQ=255)                                                                                                  acCTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCAt                        <  1:525578/140‑1 (MQ=255)                                                                                                    cTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCt                                  <  2:607599/128‑1 (MQ=255)                                                                                                    cTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCt                                  >  1:607599/1‑128 (MQ=255)                                                                                                    cTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGt                      >  2:380235/1‑140 (MQ=255)                                                                                                    cTGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCAGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGt                      <  1:390583/140‑1 (MQ=255)                                                                                                     tGGCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTc                     <  1:305083/140‑1 (MQ=255)                                                                                                       gCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCt                   <  1:715445/140‑1 (MQ=255)                                                                                                       gCAGCCCATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCt                   >  2:652276/1‑140 (MQ=255)                                                                                                           cccATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAtt               >  2:268766/1‑140 (MQ=255)                                                                                                           cccATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAtt               >  2:555258/1‑140 (MQ=255)                                                                                                           cccATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAtt               >  2:777849/1‑140 (MQ=255)                                                                                                             cATCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtgt             >  1:289126/1‑140 (MQ=255)                                                                                                              aTCAGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtgtg            >  2:969121/1‑140 (MQ=255)                                                                                                              aTCAGGTCTTCCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtgtg            <  1:684458/140‑1 (MQ=255)                                                                                                                 aGGTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTgtggtg         >  2:171584/1‑140 (MQ=255)                                                                                                                   gTCTTTCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCt       <  1:171584/140‑1 (MQ=255)                                                                                                                        tCCAGTAGCAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccag  >  2:54578/1‑140 (MQ=255)                                                                                                                             tagcagtCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttcc    <  1:711220/133‑1 (MQ=255)                                                                                                                                |                                                                                                                                    CGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAG  >  NC_000913/2745475‑2745733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑