Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,536	G→A	G95D (GGC→GAC)	gabP →	4‑aminobutyrate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,536	0	G	A	100.0%	83.6 / NA	26	G95D (GGC→GAC)	gabP	4‑aminobutyrate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/17);  total (9/17)

CTATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCA  >  NC_000913/2794402‑2794670                                                                                                                                       |                                                                                                                                       cTATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGC‑GGCTATACCATCGACTGGtt                                                                                                                                  <  1:30512/140‑1 (MQ=255)                gCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGtttt                                                                                                                    <  1:470215/140‑1 (MQ=255)                  tACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGGTGCCCCCCCCCATACCGGGTCGTTTTTCCCCCATTCCGATAAAACCATTGGTCGCTGGGCGGGCTATACCATCCACTGGGTGTACTGGTGGgttttg                                                                                                                  >  2:70399/1‑138 (MQ=255)                         ggtTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGCTACCGGTTCGGTTTCCACCCATGCCGATAAAACCATTGGTCGCTGGGCGGGGTATACCATCGACTGGTTGTACTGGTGGGTTTGGGGACTgg                                                                                                           >  2:363408/1‑140 (MQ=255)                          gtTATGATTATGCGGATGTTGGCGGAAATGGCGGGTGTCACGCCCGAAACCGGGTTGTTTTTCCCCTTTGACGCTAAAGACATTGGTCGCCGGGGGGGGCATATCAACGACTGGGTTTACTGGGGGGTTTTGGGACTggg                                                                                                          >  2:52252/1‑139 (MQ=255)                          gtTATGATTATGCGGATGTTGGCGGAAATGGCGGGTGGCCCGCCCCATACCGGGTTGTTTTCCCCCTCATCCTGTAAAAACATTTGCGGCCGGGCGGGGTGTACCATCGGCTGGTTGTACAGGTGGGTTTGGGGTCTggg                                                                                                          >  2:949481/1‑139 (MQ=255)                                              ggCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACa                                                                                      <  1:363408/140‑1 (MQ=255)                                                cGGAAATGGCGGTTGCCCCGCCCCATACCGGGTCGTTTTTCACCCATTCCGGTAAAGCCATTGGGCGCTGGGCGGGCTATACCATCGACTGGGTGTACTGGTGGGTTTGGGGACTGGTTTTTCCCCCGGAAGCCAACATc                                                                                    >  2:513682/1‑140 (MQ=255)                                                 ggAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATcg                                                                                   <  1:70399/140‑1 (MQ=255)                                                  gAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATcgc                                                                                  <  1:513917/140‑1 (MQ=255)                                                              gCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCt                                                                      <  2:268522/140‑1 (MQ=255)                                                                  cGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCAc                                                                  >  1:38616/1‑140 (MQ=255)                                                                     ccGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCa                                                                             <  1:513574/126‑1 (MQ=255)                                                                     ccGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCa                                                                             >  2:513574/1‑126 (MQ=255)                                                                       gATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATg                                                             <  2:775911/140‑1 (MQ=255)                                                                           ccGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGtt                                                         <  2:247935/140‑1 (MQ=255)                                                                              gTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCa                                                      >  1:874881/1‑140 (MQ=255)                                                                               ttCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAg                                                     >  1:816457/1‑140 (MQ=255)                                                                                  gTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCa                                                  <  2:874881/140‑1 (MQ=255)                                                                                   ttttCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCAt                                                 >  2:89212/1‑140 (MQ=255)                                                                                        cACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCa                                            >  1:466306/1‑140 (MQ=255)                                                                                         aCCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCAt                                           <  1:52252/140‑1 (MQ=255)                                                                                           cTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCt                                         <  2:466306/140‑1 (MQ=255)                                                                                                 cGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTAt                                   <  1:949481/140‑1 (MQ=255)                                                                                                       aGCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                             <  2:816457/140‑1 (MQ=255)                                                                                                        gCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                             <  1:738327/139‑1 (MQ=255)                                                                                                        gCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                             >  2:738327/1‑139 (MQ=255)                                                                                                        gCCATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCt                            >  1:769104/1‑140 (MQ=255)                                                                                                         ccATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                             >  1:766703/1‑138 (MQ=255)                                                                                                         ccATTGGTCGCTGGGCGGGCTATACCATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                             <  2:766703/138‑1 (MQ=255)                                                                                                                                  cATCGACTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCa  <  2:769104/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CTATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCA  >  NC_000913/2794402‑2794670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑