Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,854,287	T→C	intergenic (+23/‑51)	hypE → / → fhlA	hydrogenase maturation protein, carbamoyl dehydratase/DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,854,287	0	T	C	100.0%	147.7 / NA	42	intergenic (+23/‑51)	hypE/fhlA	hydrogenase maturation protein, carbamoyl dehydratase/DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/24);  total (18/24)

CGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTC  >  NC_000913/2854152‑2854423                                                                                                                                        |                                                                                                                                         cggcgCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTAtagctagt                                                                                                                                      >  2:32142/1‑140 (MQ=255) cggcgCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTAtagctagt                                                                                                                                      >  2:628837/1‑140 (MQ=255)           tGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCtt                                                                                                                            <  1:20122/140‑1 (MQ=255)                      tggAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCtt                                                                                                                 >  2:751670/1‑140 (MQ=255)                       ggAACGTAAAGGTGTTCGTCTTGCCGGTCCGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCttt                                                                                                                <  1:131240/140‑1 (MQ=255)                        gAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTg                                                                                                               <  1:66634/140‑1 (MQ=255)                             tAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCAc                                                                                                                      >  2:941698/1‑128 (MQ=255)                             tAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCAc                                                                                                                      <  1:941698/128‑1 (MQ=255)                                            tGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAAc                                                                                           >  1:463551/1‑140 (MQ=255)                                               cGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACaaa                                                                                        <  2:104461/140‑1 (MQ=255)                                                  tCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATg                                                                                     >  1:585928/1‑140 (MQ=255)                                                      tATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGC‑TTGCGGTGCTTTCCTGGAAGAACAAAATGTCata                                                                                >  1:310850/1‑140 (MQ=255)                                                           cGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATaca                                                                            <  2:580427/140‑1 (MQ=255)                                                           cGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATaca                                                                            >  2:455356/1‑140 (MQ=255)                                                                aaCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAAt                                                                                      <  2:337039/125‑1 (MQ=255)                                                                aaCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAAt                                                                                      >  1:337039/1‑125 (MQ=255)                                                                aaCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGAt                                                                       <  2:585928/140‑1 (MQ=255)                                                                                aCCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAg                                                                    <  1:911007/127‑1 (MQ=255)                                                                                aCCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAg                                                                    >  2:911007/1‑127 (MQ=255)                                                                                aCCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGacaa                                                       <  2:923/140‑1 (MQ=255)                                                                                 ccACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCgg                                                           <  2:343974/135‑1 (MQ=255)                                                                                 ccACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCgg                                                           >  1:343974/1‑135 (MQ=255)                                                                                  cacaCGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGacaaca                                                     <  2:463551/140‑1 (MQ=255)                                                                                     acGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGC‑TTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAggg                                                 <  2:310850/140‑1 (MQ=255)                                                                                       gCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                            >  2:127760/1‑128 (MQ=255)                                                                                       gCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                            >  2:413803/1‑128 (MQ=255)                                                                                       gCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                            <  1:127760/128‑1 (MQ=255)                                                                                       gCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                            <  1:413803/128‑1 (MQ=255)                                                                                       gCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAggg                                                 <  2:230382/139‑1 (MQ=255)                                                                                       gCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAggg                                                 >  1:230382/1‑139 (MQ=255)                                                                                         cGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGgttg                                              <  1:751670/140‑1 (MQ=255)                                                                                           aaCCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGgttgtt                                            <  2:26046/140‑1 (MQ=255)                                                                                                 ttCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACAt                                      <  1:455356/140‑1 (MQ=255)                                                                                                 ttCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACAt                                      >  2:740435/1‑140 (MQ=255)                                                                                                     gcgTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACt                                  >  2:723340/1‑140 (MQ=255)                                                                                                     gcgTATATGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGACCTCGGACAACAAGGGTTGTTCGACATCACt                                  <  1:628837/140‑1 (MQ=255)                                                                                                          tatGCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGac                             >  2:167806/1‑140 (MQ=255)                                                                                                             gCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCAc                                   >  1:962933/1‑131 (MQ=255)                                                                                                             gCTAATAAAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCAc                                   <  2:962933/131‑1 (MQ=255)                                                                                                                 ataaAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGac                             <  2:606115/133‑1 (MQ=255)                                                                                                                 ataaAATTCTAAATCTCCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGac                             >  1:606115/1‑133 (MQ=255)                                                                                                                                tcCTATAGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTg       <  1:844120/140‑1 (MQ=255)                                                                                                                                    ataGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGcc              >  2:347464/1‑129 (MQ=255)                                                                                                                                    ataGCTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGcc              <  1:347464/129‑1 (MQ=255)                                                                                                                                     tagctagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTc  <  1:723340/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTC  >  NC_000913/2854152‑2854423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑