| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,902,161 | T→C | L89S (TTA→TCA) | ygcE → | putative sugar kinase YgcE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,902,161 | 0 | T | C | 96.4% | 182.7 / ‑2.1 | 56 | L89S (TTA→TCA) | ygcE | putative sugar kinase YgcE |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (29/25); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
GCAGAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAAC > NC_000913/2902031‑2902296 | gCAGAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGc > 1:444518/1‑140 (MQ=255) cAGAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGcc > 1:971142/1‑140 (MQ=255) cAGAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGcc > 1:361862/1‑140 (MQ=255) aGAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCg < 2:652830/140‑1 (MQ=255) gAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGa > 1:83443/1‑140 (MQ=255) cATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTTACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATgg < 2:615722/140‑1 (MQ=255) ccTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCAc < 2:83443/140‑1 (MQ=255) acgaTTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCctgct > 1:74069/1‑140 (MQ=255) catcatTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGAtt > 1:903828/1‑140 (MQ=255) tcatTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAg < 2:971142/140‑1 (MQ=255) catTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGc > 2:389579/1‑140 (MQ=255) catTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGa > 1:487222/1‑135 (MQ=255) catTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGa < 2:487222/135‑1 (MQ=255) atTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCAGATGGCACGCCTGCTGCGCCGTTGATTAGCt < 1:362947/140‑1 (MQ=255) gTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAg > 2:659719/1‑140 (MQ=255) ttttGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAgg < 2:444518/140‑1 (MQ=255) tGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATg > 2:218205/1‑140 (MQ=255) tGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGAAGGATg > 2:268835/1‑140 (MQ=255) ccGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCa < 1:218205/140‑1 (MQ=255) ggTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCAcg < 1:659719/140‑1 (MQ=255) ggTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGAAGGATGCAcg < 1:268835/140‑1 (MQ=255) aTTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTaca < 2:903828/140‑1 (MQ=255) aTTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTaca < 2:74069/140‑1 (MQ=255) aTTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATACACGCGTTaca < 2:651319/140‑1 (MQ=255) gatgaGTCAGTTTTCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGcc < 1:538256/140‑1 (MQ=255) aGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGaa < 1:389579/140‑1 (MQ=255) tGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacaca > 1:607693/1‑140 (MQ=255) gCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacacac < 2:798671/140‑1 (MQ=255) gggAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTaa > 1:937015/1‑140 (MQ=255) aTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACg > 2:28429/1‑126 (MQ=255) aTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACg < 1:28429/126‑1 (MQ=255) aTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACg > 1:436955/1‑126 (MQ=255) aTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACg < 2:436955/126‑1 (MQ=255) atatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCa > 1:693747/1‑140 (MQ=255) atTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc < 1:490150/128‑1 (MQ=255) atTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc > 2:490150/1‑128 (MQ=255) atTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACg > 2:791729/1‑133 (MQ=255) atTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACg < 1:791729/133‑1 (MQ=255) tGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATg > 1:86136/1‑140 (MQ=255) tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTgg < 2:974007/128‑1 (MQ=255) tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTgg > 1:974007/1‑128 (MQ=255) tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCa > 2:417660/1‑130 (MQ=255) tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCa < 1:417660/130‑1 (MQ=255) gTCTGGGATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCtttt > 1:832769/1‑140 (MQ=255) ggATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg < 1:82754/140‑1 (MQ=255) ggATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg < 2:937015/140‑1 (MQ=255) ggATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg < 1:42659/140‑1 (MQ=255) ggATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg > 2:359325/1‑140 (MQ=255) ggATCCATCCGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg > 2:324946/1‑140 (MQ=255) catccGTTGCTGCCGTGCGTCATTGAAAGCCGATGGTACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATc > 2:464800/1‑140 (MQ=255) catccGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATc > 2:213212/1‑140 (MQ=255) catccGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATc > 2:703956/1‑140 (MQ=255) atccGTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCt > 1:848407/1‑140 (MQ=255) gTTGCTGCCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACg < 2:693747/140‑1 (MQ=255) tgcCGTGCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCg > 2:651447/1‑140 (MQ=255) gCGTCATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAAc > 1:251447/1‑140 (MQ=255) | GCAGAACATCCTGACGACGATTTATGGGCATCATTATGTTTTGCCGGTCACGATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAAC > NC_000913/2902031‑2902296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |