Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,917,723	T→C	G889G (GGT→GGC)	barA →	sensory histidine kinase BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,917,723	0	T	C	100.0%	169.2 / NA	48	G889G (GGT→GGC)	barA	sensory histidine kinase BarA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/27);  total (21/27)

GTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTT  >  NC_000913/2917586‑2917858                                                                                                                                          |                                                                                                                                        gTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCAc                                                                                                                                       <  2:866497/140‑1 (MQ=255)     aGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTaaa                                                                                                                                   >  2:595350/1‑140 (MQ=255)        aaCAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAaagaa                                                                                                                                <  2:228869/140‑1 (MQ=255)            gCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAaagaagaag                                                                                                                            >  2:75011/1‑140 (MQ=255)            gCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAaagaagaag                                                                                                                            >  2:335465/1‑140 (MQ=255)                gTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGAttt                                                                                                                        >  2:274687/1‑140 (MQ=255)                         aaCCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGa                                                                                                               <  1:385457/140‑1 (MQ=255)                          aCCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAg                                                                                                              >  1:917082/1‑140 (MQ=255)                               gAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAgctgct                                                                                                         >  2:836543/1‑140 (MQ=255)                                aaGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGctgctg                                                                                                        <  1:491129/140‑1 (MQ=255)                                 aGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTgg                                                                                                       >  2:299069/1‑140 (MQ=255)                                    ccTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTgg                                                                                                       >  2:137219/1‑137 (MQ=255)                                    ccTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTgg                                                                                                       <  1:137219/137‑1 (MQ=255)                                    ccTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAAc                                                                                                    >  1:482287/1‑140 (MQ=255)                                      tGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTg                                                                                                  <  2:315731/140‑1 (MQ=255)                                          tgatttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTgg                                                                                              <  2:482287/140‑1 (MQ=255)                                          tgatttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTgg                                                                                              <  2:637658/140‑1 (MQ=255)                                           gatttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                                                             <  1:497877/140‑1 (MQ=255)                                           gatttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                                                             <  1:724347/140‑1 (MQ=255)                                            atttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc                                                                                            <  2:222111/140‑1 (MQ=255)                                             tttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                                                             >  2:39932/1‑138 (MQ=255)                                             tttgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                                                             <  1:39932/138‑1 (MQ=255)                                               tgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag                                                                                         <  2:559536/140‑1 (MQ=255)                                               tgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag                                                                                         <  2:221056/140‑1 (MQ=255)                                               tgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag                                                                                         <  1:262197/140‑1 (MQ=255)                                               tgattCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag                                                                                         <  1:299069/140‑1 (MQ=255)                                                  ttCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTg                                                                                                  <  2:128608/128‑1 (MQ=255)                                                  ttCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTg                                                                                                  >  1:128608/1‑128 (MQ=255)                                                  ttCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATg                                                                                      <  1:274687/140‑1 (MQ=255)                                                                          gCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGc                                                              >  1:748847/1‑140 (MQ=255)                                                                                  ggTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtct                                                      <  2:917082/140‑1 (MQ=255)                                                                                        ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTa                                                >  1:469190/1‑140 (MQ=255)                                                                                             tATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGa                                           >  1:68062/1‑140 (MQ=255)                                                                                              aTGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGAt                                          <  2:519456/140‑1 (MQ=255)                                                                                              aTGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGAt                                          >  2:842017/1‑140 (MQ=255)                                                                                              aTGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGACCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGAt                                          <  2:439667/140‑1 (MQ=255)                                                                                                gaagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGt                                        >  1:774295/1‑140 (MQ=255)                                                                                                                cTGATCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAAt                        <  2:774295/140‑1 (MQ=255)                                                                                                                   aTCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAg                               <  1:323995/130‑1 (MQ=255)                                                                                                                   aTCGAACAACAGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAg                               >  2:323995/1‑130 (MQ=255)                                                                                                                             aGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTa              >  1:401517/1‑137 (MQ=255)                                                                                                                             aGCTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTa              <  2:401517/137‑1 (MQ=255)                                                                                                                               cTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACg         <  1:355703/140‑1 (MQ=255)                                                                                                                               cTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACg         <  1:335465/140‑1 (MQ=255)                                                                                                                               cTACGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACg         >  2:674571/1‑140 (MQ=255)                                                                                                                                 aCGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACg         <  1:955062/138‑1 (MQ=255)                                                                                                                                 aCGTAGCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACg         >  2:955062/1‑138 (MQ=255)                                                                                                                                      gCGGCACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGttt  >  1:470318/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTT  >  NC_000913/2917586‑2917858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑