Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,495	T→C	L694L (TTG→CTG)	ybbP →	putative ABC transporter membrane subunit YbbP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,495	0	T	C	100.0%	184.3 / NA	52	L694L (TTG→CTG)	ybbP	putative ABC transporter membrane subunit YbbP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

ACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTC  >  NC_000913/522360‑522631                                                                                                                                        |                                                                                                                                         acacAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTAtgctgct                                                                                                                                      <  1:782029/140‑1 (MQ=255)  cacaACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTAtgctgctg                                                                                                                                     >  1:377555/1‑140 (MQ=255)       cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATgctgctgttgct                                                                                                                                >  1:786673/1‑140 (MQ=255)                cAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCAcaggt                                                                                                                       <  1:328355/140‑1 (MQ=255)                 aGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCAcaggtg                                                                                                                      >  1:486502/1‑140 (MQ=255)                  gTTCCCGACCATTAGCTTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCAcaggtgc                                                                                                                     <  2:256206/140‑1 (MQ=255)                      ccGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCAcaggtgcaggt                                                                                                                 <  2:662824/140‑1 (MQ=255)                         aCCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTggg                                                                                                              <  2:757989/140‑1 (MQ=255)                             ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATg                                                                                                          >  2:548552/1‑140 (MQ=255)                                    gTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCAcaggtgcagg                                                                                                                  >  2:641871/1‑125 (MQ=255)                                    gTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCAcaggtgcagg                                                                                                                  <  1:641871/125‑1 (MQ=255)                                    gTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagc                                                                                                   >  1:158546/1‑140 (MQ=255)                                    gTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagc                                                                                                   >  2:697047/1‑140 (MQ=255)                                     ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGCGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                                                                  >  2:113060/1‑140 (MQ=255)                                          tatTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatc                                                                                             >  2:544039/1‑140 (MQ=255)                                            ttGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAg                                                                                           >  1:778495/1‑140 (MQ=255)                                               gtgcgATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAg                                                                                        >  1:755614/3‑140 (MQ=255)                                               gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAg                                                                                        >  2:122623/1‑140 (MQ=255)                                               gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAg                                                                                        >  1:586765/1‑140 (MQ=255)                                                    aTTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtggt                                                                                   >  1:450431/1‑140 (MQ=255)                                                     ttttAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTACGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtggtg                                                                                  <  2:683299/140‑1 (MQ=255)                                                       ttAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                                                                <  1:544039/140‑1 (MQ=255)                                                             cAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcg                                                                          <  1:697047/140‑1 (MQ=255)                                                              aGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcgc                                                                         <  2:724250/140‑1 (MQ=255)                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGgtgt                                                                              <  2:201603/134‑1 (MQ=255)                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGgtgt                                                                              >  1:201603/1‑134 (MQ=255)                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                                                        >  1:868761/1‑140 (MQ=255)                                                                gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGcac                                                                       >  2:599171/1‑140 (MQ=255)                                                                 tcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGcaca                                                                      >  2:289278/1‑140 (MQ=255)                                                                         gTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGc                                                              <  1:548552/140‑1 (MQ=255)                                                                              ggAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGa                                                         <  1:465427/140‑1 (MQ=255)                                                                                   aGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaaaaaa                                                    <  1:113060/140‑1 (MQ=255)                                                                                   aGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaaaaaa                                                    <  1:289278/140‑1 (MQ=255)                                                                                    ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAAc                                                   >  1:76074/1‑140 (MQ=255)                                                                                        aGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct                                               <  2:778495/140‑1 (MQ=255)                                                                                             ggCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTa                                          <  1:352833/140‑1 (MQ=255)                                                                                               cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc                                        >  2:759241/1‑140 (MQ=255)                                                                                                     aaGTGATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTtg                                  <  1:129278/140‑1 (MQ=255)                                                                                                         gATGGTGGTACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTgtggt                              <  1:122623/140‑1 (MQ=255)                                                                                                                gtACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc                                        >  2:366407/1‑123 (MQ=255)                                                                                                                gtACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc                                        <  1:968620/123‑1 (MQ=255)                                                                                                                gtACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc                                        <  1:366407/123‑1 (MQ=255)                                                                                                                gtACTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc                                        >  2:968620/1‑123 (MQ=255)                                                                                                                  aCTGGTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCg                     >  2:747807/1‑140 (MQ=255)                                                                                                                     ggTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCa                  <  2:158546/140‑1 (MQ=255)                                                                                                                      gTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCAt                 >  2:645193/1‑140 (MQ=255)                                                                                                                      gTCACCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCAt                 <  1:645193/140‑1 (MQ=255)                                                                                                                         aCCGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCt              >  1:186232/1‑140 (MQ=255)                                                                                                                           cGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGcc                   >  2:627979/1‑133 (MQ=255)                                                                                                                           cGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGcc                   <  1:627979/133‑1 (MQ=255)                                                                                                                           cGCCTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTg            <  1:747807/140‑1 (MQ=255)                                                                                                                             ccTGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTggg          >  2:751081/1‑140 (MQ=255)                                                                                                                               tGCGGTATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGgtt        >  1:146382/1‑140 (MQ=255)                                                                                                                                    tATGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGgtttgttt   <  1:759241/140‑1 (MQ=255)                                                                                                                                     aTGCTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTc  <  2:486502/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTTGTGGTGTGAGTTCGCCATGCTTGGGTTTGTTTC  >  NC_000913/522360‑522631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑