Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,485	T→C	G142G (GGT→GGC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,485	0	T	C	100.0%	136.8 / NA	39	G142G (GGT→GGC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/16);  total (23/16)

GATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACG  >  NC_000913/3016354‑3016614                                                                                                                                    |                                                                                                                                  gATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCGCCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGtt                                                                                                                           <  1:860516/140‑1 (MQ=255)  aTGCCGAAGACTAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTc                                                                                                                          <  2:963369/140‑1 (MQ=255)  aTGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGtt                                                                                                                           >  1:127948/1‑139 (MQ=255)  aTGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGtt                                                                                                                           >  2:469902/1‑139 (MQ=255)  aTGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGtt                                                                                                                           <  2:127948/139‑1 (MQ=255)  aTGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGtt                                                                                                                           <  1:469902/139‑1 (MQ=255)  aTGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTc                                                                                                                          <  1:134147/140‑1 (MQ=255)  aTGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTc                                                                                                                          <  2:470108/140‑1 (MQ=255)   tGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCa                                                                                                                         <  1:89101/140‑1 (MQ=255)   tGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCa                                                                                                                         <  2:804308/140‑1 (MQ=255)   tGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCa                                                                                                                         <  2:261122/140‑1 (MQ=255)            cGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAata                                                                                                                <  2:991113/140‑1 (MQ=255)                          ccGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTAc                                                                                                  <  1:266379/140‑1 (MQ=255)                            gAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACaa                                                                                                >  1:33078/1‑140 (MQ=255)                            gAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACaa                                                                                                >  2:413227/1‑140 (MQ=255)                            gAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGACTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACaa                                                                                                >  1:883679/1‑140 (MQ=255)                                ggTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGACTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTc                                                                                            <  2:883679/140‑1 (MQ=255)                                  tCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGa                                                                                          <  1:413227/140‑1 (MQ=255)                                  tCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGa                                                                                          <  1:483672/140‑1 (MQ=255)                                       cGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTa                                                                                     >  2:718416/1‑140 (MQ=255)                                           tttACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTaa                                                                                 >  1:354717/1‑140 (MQ=255)                                                    cTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGa                                                                                          >  2:677761/1‑122 (MQ=255)                                                    cTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGa                                                                                          <  1:677761/122‑1 (MQ=255)                                                    cTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAAccgcc                                                                        >  2:135621/1‑140 (MQ=255)                                                     ttAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAAccgccg                                                                       >  1:310603/1‑140 (MQ=255)                                                              gggATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGAtctgtctc                                                              >  1:967847/1‑133 (MQ=255)                                                              gggATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAg                                                              >  1:725886/1‑140 (MQ=255)                                                              gggATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCAGATGCAACAg                                                              >  1:359214/1‑140 (MQ=255)                                                                    aaTACCTCAAGCCTTGTTTTCCCCTCCCCTTTCTCGAAGGGTTTTTCCGCCTTTCTGTTTCGGAAAAATCTTTCATTTTAAATAGGAGCGTCGTTTACACCCTGGAAGTTATAAAGCAACCGCCGATGCAACAGTTCATc                                                        <  2:184862/140‑1 (MQ=255)                                                                     aTACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCg                                                       >  1:326868/1‑140 (MQ=255)                                                                     aTACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCg                                                       >  1:655081/1‑140 (MQ=255)                                                                        cccaaaaGCTTGTTTCCCCCTCCCCCTTCTCGAAGCGTTTTCCCACCCTTCTGTTCCCGAAAAATCTTTCATTTTTAATAGGGGCGTGGTTTACACCCTAGAAGTTATAAACCAACCGCCGATGCAACAGTTCATCGACa                                                    <  2:326868/136‑1 (MQ=255)                                                                         cTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACaa                                                   >  1:184862/1‑140 (MQ=255)                                                                              aGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATatga                                              >  2:614741/1‑140 (MQ=255)                                                                                              cACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCa                              >  1:102280/1‑140 (MQ=255)                                                                                                        aaGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTc                    >  1:428556/1‑140 (MQ=255)                                                                                                        aaGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTc                    >  1:711667/1‑140 (MQ=255)                                                                                                           cGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCAGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCt                 <  2:359214/140‑1 (MQ=255)                                                                                                            gATTTTCCACCTTTCTGATTCCGGAAAATCTTTCATTTTTAATATGAGCGTGGGTTACACCCTCGAAGGTATAAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTc                <  2:310603/140‑1 (MQ=255)                                                                                                                   cAGCCTTCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATcg         >  2:354479/1‑140 (MQ=255)                                                                                                                        ttCTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATa    >  1:724341/1‑140 (MQ=255)                                                                                                                          cTGATTCCGGCAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACg  >  2:817941/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                  GATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACG  >  NC_000913/3016354‑3016614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑