Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,034,537	A→G	V213A (GTG→GCG)	lysS ←	lysine‑‑tRNA ligase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,034,537	0	A	G	100.0%	180.4 / NA	52	V213A (GTG→GCG)	lysS	lysine‑‑tRNA ligase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

GATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGA  >  NC_000913/3034401‑3034671                                                                                                                                         |                                                                                                                                       gATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCt                                                                                                                                     <  2:376984/140‑1 (MQ=255)            cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGc                                                                                                                          <  1:278922/140‑1 (MQ=255)                   gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCaa                                                                                                                   >  1:613907/1‑140 (MQ=255)                           caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATa                                                                                                           >  1:762314/1‑140 (MQ=255)                                 ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCcg                                                                                                     >  1:280754/1‑140 (MQ=255)                                     aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGt                                                                                                 >  1:540518/1‑140 (MQ=255)                                     aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGt                                                                                                 >  2:716223/1‑140 (MQ=255)                                       gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTAATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGt                                                                                                 >  1:191327/1‑138 (MQ=255)                                       gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTAATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGt                                                                                                 <  2:191327/138‑1 (MQ=255)                                         ttGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCcg                                                                                                     <  2:338685/132‑1 (MQ=255)                                         ttGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCcg                                                                                                     >  1:338685/1‑132 (MQ=255)                                           gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCa                                                                                           >  2:398072/1‑140 (MQ=255)                                             ggTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATg                                                                                         <  2:280754/140‑1 (MQ=255)                                                 cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACt                                                                                     >  1:447807/1‑140 (MQ=255)                                                   gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTgg                                                                                   >  1:253549/1‑140 (MQ=255)                                                     tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAATCCGCGGTTCACCATGAACTGGCg                                                                                 >  2:264028/1‑140 (MQ=255)                                                     tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg                                                                                 <  2:540518/140‑1 (MQ=255)                                                      ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa                                                                                >  1:881836/1‑140 (MQ=255)                                                       cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa                                                                               <  2:613907/140‑1 (MQ=255)                                                        ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAAt                                                                              >  1:535820/1‑140 (MQ=255)                                                        ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAAt                                                                              <  2:535820/140‑1 (MQ=255)                                                              aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa                                                                                <  2:751771/132‑1 (MQ=255)                                                              aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa                                                                                >  1:751771/1‑132 (MQ=255)                                                                 cGCAGGTACATATCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGc                                                                                  >  1:432564/1‑127 (MQ=255)                                                                 cGCAGGTACATATCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGc                                                                                  <  2:432564/127‑1 (MQ=255)                                                                  gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAgg                                                                    <  2:253549/140‑1 (MQ=255)                                                                       tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTg                                                               >  1:950686/1‑140 (MQ=255)                                                                         cATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCg                                                             >  2:479839/1‑140 (MQ=255)                                                                           tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGGATACCAGAGAGGATCTGCGAg                                                           >  2:908883/1‑140 (MQ=255)                                                                           tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAg                                                           <  1:908883/140‑1 (MQ=255)                                                                             tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAATCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg                                                         <  1:264028/140‑1 (MQ=255)                                                                             tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg                                                         <  2:762314/140‑1 (MQ=255)                                                                             tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCCGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg                                                         <  1:398072/140‑1 (MQ=255)                                                                                agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCAc                                                      >  1:261049/1‑140 (MQ=255)                                                                                agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCAc                                                      <  1:222488/140‑1 (MQ=255)                                                                                        cgcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAgg                                              >  2:617156/1‑140 (MQ=255)                                                                                           gTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGgtgt                                           >  1:108281/1‑140 (MQ=255)                                                                                            ttATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGtt                                          >  1:85991/1‑140 (MQ=255)                                                                                               tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCg                                       <  1:617156/140‑1 (MQ=255)                                                                                               tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCg                                       >  2:561676/1‑140 (MQ=255)                                                                                                     gtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTc                                 >  1:551929/1‑140 (MQ=255)                                                                                                             cGGACGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTgg                         <  1:230705/140‑1 (MQ=255)                                                                                                                aCGCGCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgaga                      >  2:587032/1‑140 (MQ=255)                                                                                                                 cgcgCAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGATAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgagat                     <  1:538351/140‑1 (MQ=255)                                                                                                                     cAGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgagatgaga                 <  1:956228/140‑1 (MQ=255)                                                                                                                      aGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgagatgagat                >  1:819810/1‑140 (MQ=255)                                                                                                                      aGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgagatgagat                <  1:716223/140‑1 (MQ=255)                                                                                                                      aGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgagatgagat                <  1:561676/140‑1 (MQ=255)                                                                                                                      aGCGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGgagatgagat                <  1:450722/140‑1 (MQ=255)                                                                                                                        cGGCACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCg              >  2:827519/1‑140 (MQ=255)                                                                                                                           cACCGCCAGGGATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgaga           <  1:479839/140‑1 (MQ=255)                                                                                                                                    ggATCGCCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGa  <  2:950686/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGA  >  NC_000913/3034401‑3034671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑