| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,042,574 | T→C | Y192C (TAC→TGC) | yqfA ← | hemolysin‑III family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,042,574 | 0 | T | C | 97.6% | 136.8 / ‑4.9 | 42 | Y192C (TAC→TGC) | yqfA | hemolysin‑III family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (28/13); total (29/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGC > NC_000913/3042449‑3042710 | gACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTg < 2:199184/140‑1 (MQ=255) gcgcGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGt > 1:151615/1‑140 (MQ=255) cgcgTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTa < 1:529230/140‑1 (MQ=255) tCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGaaa > 1:618309/1‑140 (MQ=255) tCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCAc > 2:705207/1‑140 (MQ=255) ccTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGa < 2:618309/140‑1 (MQ=255) cTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAg > 1:734397/1‑140 (MQ=255) cTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAg > 1:188527/1‑140 (MQ=255) aCAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCg > 1:555688/1‑140 (MQ=255) gCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATaaa > 1:110557/1‑140 (MQ=255) gCATACCCATTACGCCTGCCCAATATACAAA‑AGAACCCCAGAAAGTGGCAAAAACCACCGCCCAGCACGAAACCGTGGCAGAATGCAAGGTTGCATGGGA‑TGGGGTTGCAAACGTAAAAAAACAACCCGAGCGAATAAAc > 2:327551/1‑140 (MQ=255) cATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAAc > 1:350645/1‑140 (MQ=255) tACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCa > 1:593496/1‑140 (MQ=255) aCCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGa > 1:250029/1‑131 (MQ=255) aCCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGa < 2:250029/131‑1 (MQ=255) aCCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAc < 1:612979/140‑1 (MQ=255) cccATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcg < 2:614479/140‑1 (MQ=255) ccATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcgc < 2:151615/140‑1 (MQ=255) ttACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcgccgc < 2:734397/140‑1 (MQ=255) tACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcg > 2:983212/1‑135 (MQ=255) tACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCAcg < 1:983212/135‑1 (MQ=255) tACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACgccgcc > 1:457320/1‑140 (MQ=255) cccTCCCAAATATAAAAATAGATCGCAAGAAGGTGGCCCCCCCTCCCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCCCCCCGAGCGAATAACCCACGCCGCCTAc < 2:188527/139‑1 (MQ=255) ccTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCCCCCCGAGCGAATAAACCACGCCGCCTAcc < 1:382024/140‑1 (MQ=255) cccAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCa > 1:88899/1‑140 (MQ=255) cccAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCa > 2:670360/1‑140 (MQ=255) ccAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGACAg > 1:945530/1‑140 (MQ=255) ccAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGACAg < 2:945530/140‑1 (MQ=255) caaaTATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAg < 1:670360/138‑1 (MQ=255) aaTATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTa < 1:705207/140‑1 (MQ=255) gATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGc > 2:235612/1‑140 (MQ=255) aTCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGcc > 2:966109/1‑140 (MQ=255) tCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGccc > 1:730161/1‑140 (MQ=255) tCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGccc > 1:857730/1‑140 (MQ=255) cGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCccg > 2:24690/1‑140 (MQ=255) tACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCAtt > 2:339980/1‑140 (MQ=255) ccgccgAGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTc > 1:819070/1‑140 (MQ=255) aGCACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAAt > 1:304768/1‑140 (MQ=255) cACGAAGCCGTGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAACCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTa > 2:187142/1‑140 (MQ=255) tGCCAGATGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGa > 2:814214/1‑140 (MQ=255) tGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagccag > 1:512012/1‑140 (MQ=255) tGGCATGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagccag > 1:337299/1‑140 (MQ=255) gcgggTTGGAAGGAATTGCTTTTGCAGAGGAAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACagccagcc < 2:88899/137‑1 (MQ=255) aTGGTTGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCa > 1:572273/1‑140 (MQ=255) ttGCATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGc > 2:727903/1‑140 (MQ=255) | GACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAA‑TGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGC > NC_000913/3042449‑3042710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |