Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,026	(C)6→5	coding (627/1179 nt)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,021	0	C	.	100.0%	145.4 / NA	35	coding (632/1179 nt)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (18/17);  total (18/17)

ACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTAC  >  NC_000913/2637883‑2638153                                                                                                                                           |                                                                                                                                     acGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGcc                                                                                                                                     <  1:18611/140‑1 (MQ=255) acGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGcc                                                                                                                                     <  2:567808/140‑1 (MQ=255)        tAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGcccccacga                                                                                                                              >  2:803493/1‑136 (MQ=255)                acgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCa                                                                                                                     >  1:595333/1‑140 (MQ=255)                 cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCg                                                                                                                       >  1:440147/1‑137 (MQ=255)                 cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCg                                                                                                                       <  2:440147/137‑1 (MQ=255)                 cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCAc                                                                                                                    >  1:523125/1‑140 (MQ=255)                         gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGcc                                                                                                            >  2:963578/1‑140 (MQ=255)                                   cGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                  >  1:57754/1‑140 (MQ=255)                                     cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCa                                                                                                >  2:165033/1‑140 (MQ=255)                                          aCGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTc                                                                                           >  2:458333/1‑140 (MQ=255)                                            ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                         <  1:165033/140‑1 (MQ=255)                                            ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                         <  2:969192/140‑1 (MQ=255)                                            ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCACTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                         <  2:732609/140‑1 (MQ=255)                                             gTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                                        >  1:117556/1‑140 (MQ=255)                                             gTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                                        >  1:977200/1‑140 (MQ=255)                                                 aTTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGc                                                                                    <  2:57754/140‑1 (MQ=255)                                                  tttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                                                                   <  2:117575/140‑1 (MQ=255)                                                       gTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagag                                                                              >  2:329261/1‑140 (MQ=255)                                                           aaCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑TCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCg                                                                          <  2:679792/140‑1 (MQ=255)                                                           aaCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCg                                                                          <  2:595333/140‑1 (MQ=255)                                                            aCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCACACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGa                                                                         >  2:312755/1‑140 (MQ=255)                                                                     cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                                                                >  2:547441/1‑140 (MQ=255)                                                                        ggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAg                                                             <  2:551073/140‑1 (MQ=255)                                                                         gAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAgg                                                            >  1:469457/1‑140 (MQ=255)                                                                                  tgctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                                                                <  2:339052/127‑1 (MQ=255)                                                                                  tgctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                                                                >  1:339052/1‑127 (MQ=255)                                                                                  tgctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTc                                                   >  2:282654/1‑140 (MQ=255)                                                                                   gctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCACACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTcc                                                  >  2:330023/1‑140 (MQ=255)                                                                                      gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAtt                                               >  1:899375/1‑140 (MQ=255)                                                                                           atcatcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACa                                          <  1:547441/140‑1 (MQ=255)                                                                                                cTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGcc                                     <  2:899375/140‑1 (MQ=255)                                                                                                            cATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGtt                         <  2:117556/140‑1 (MQ=255)                                                                                                              tCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCa                       >  1:13657/1‑140 (MQ=255)                                                                                                               cAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg                      <  1:388597/140‑1 (MQ=255)                                                                                                               cAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCACACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg                      <  1:330023/140‑1 (MQ=255)                                                                                                                             cgcgACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCTGTCAGCTTCGTTCAGCGCTTGTAACTGAc        <  2:977200/140‑1 (MQ=255)                                                                                                                                 aCCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGtt    >  1:316933/1‑140 (MQ=255)                                                                                                                                   cATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTAc  <  2:523125/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     ACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTAC  >  NC_000913/2637883‑2638153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑