Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,890	(A)8→9	intergenic (‑25/+207)	sibD ← / ← sibE	small RNA SibD/small RNA SibE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,882	1	.	A	100.0%	127.5 / NA	37	intergenic (‑17/+215)	sibD/sibE	small RNA SibD/small RNA SibE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (24/13);  total (24/13)

GGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTG‑AAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACC  >  NC_000913/3194743‑3195008                                                                                                                                             |                                                                                                                               gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTg                                                                                                                                 >  2:61288/1‑140 (MQ=255) gggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTg                                                                                                                                 >  2:119801/1‑140 (MQ=255)       tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTG‑aaaaaa                                                                                                                          >  2:137234/1‑140 (MQ=255)             aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTG‑aaaaaaaaatca                                                                                                                    <  1:119801/140‑5 (MQ=255)                       tATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGc                                                                                                                   <  2:642300/132‑1 (MQ=255)                       tATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGc                                                                                                                   >  1:642300/1‑132 (MQ=255)                        atgatgAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCaagaag                                                                                                          >  1:326319/1‑140 (MQ=255)                         tgatgaAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAgg                                                                                                         <  2:956580/140‑1 (MQ=255)                          gatgaAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGt                                                                                                        <  2:42218/140‑1 (MQ=255)                          gatgaAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGt                                                                                                        <  2:326319/140‑1 (MQ=255)                          gatgaAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGt                                                                                                        <  2:131508/140‑1 (MQ=255)                              aaGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCa                                                                                                    >  1:125250/1‑140 (MQ=255)                              aaGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCa                                                                                                    >  1:438522/1‑140 (MQ=255)                                      catcatACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAAcaca                                                                                            <  2:327242/140‑1 (MQ=255)                                                aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGc                                                                                  >  2:377749/1‑140 (MQ=255)                                                  tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGccc                                                                                >  2:226729/1‑140 (MQ=255)                                                  tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGccc                                                                                >  2:270119/1‑140 (MQ=255)                                                  tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGG‑TTCATTAACACATATCCCTGGCCCa                                                                               >  2:367743/1‑140 (MQ=255)                                                          tACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGcc                                                                        >  1:58128/1‑140 (MQ=255)                                                              cGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCgggg                                                                    >  1:161511/1‑140 (MQ=255)                                                                   gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGAtttt                                                               >  2:729711/1‑140 (MQ=255)                                                                      tCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                                            >  2:629292/1‑140 (MQ=255)                                                                           gCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                                       >  1:343395/1‑140 (MQ=255)                                                                              tattaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg                                                    <  2:343395/140‑1 (MQ=255)                                                                                      gCCAATCAGAGGTGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGACGGATTCATTAACACCTATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                                            >  2:933887/1‑140 (MQ=38)                                                                                      gCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                                            >  1:481920/1‑140 (MQ=255)                                                                                          aTCAGAAGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAg                                        >  1:713056/1‑140 (MQ=255)                                                                                                ggggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCaa                                  <  2:481920/140‑1 (MQ=255)                                                                                                agggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCaa                                  <  2:713056/139‑1 (MQ=255)                                                                                                 gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCaaa                                 >  1:77792/1‑140 (MQ=255)                                                                                                       gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCa                           <  1:226729/140‑1 (MQ=255)                                                                                                        aaaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAg                          >  2:252387/1‑140 (MQ=255)                                                                                                             ctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGt                     >  1:204746/1‑140 (MQ=255)                                                                                                             ctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGt                     >  2:139034/1‑140 (MQ=255)                                                                                                                cctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGc                  >  1:145019/1‑140 (MQ=255)                                                                                                                 ctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa                 <  2:77792/140‑1 (MQ=255)                                                                                                                             ccTTTACTTTAGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTg     <  2:204746/140‑1 (MQ=255)                                                                                                                                ttactttaGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTg     >  2:818994/1‑137 (MQ=255)                                                                                                                                ttactttaGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTg     <  1:818994/137‑1 (MQ=255)                                                                                                                                ttactttaGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc  >  2:654174/1‑140 (MQ=255)                                                                                                                                ttactttaGGTTGAAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGAcc  >  1:908452/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                               GGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTG‑AAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACC  >  NC_000913/3194743‑3195008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑