Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,826	+G	coding (992/1245 nt)	mtr ←	tryptophan:H(+) symporter Mtr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,822	1	.	G	100.0%	181.4 / NA	52	P332P (CCG→CCC)	mtr	tryptophan:H(+) symporter Mtr
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (33/19);  total (33/19)

ATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGC‑GGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACA  >  NC_000913/3304683‑3304955                                                                                                                                              |                                                                                                                                      aTTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGc                                                                                                                                        <  1:274056/140‑1 (MQ=255)  tttCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGC‑g                                                                                                                                      <  2:86767/140‑1 (MQ=255)     cGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGC‑gggg                                                                                                                                   >  2:926618/1‑140 (MQ=255)        gCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGC‑gggggca                                                                                                                                <  1:765641/140‑4 (MQ=255)        gCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGC‑gggggca                                                                                                                                <  2:11186/140‑4 (MQ=255)                  cGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCa                                                                                                                       <  2:122574/140‑1 (MQ=255)                                aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                                         <  2:19037/140‑1 (MQ=255)                                aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                                         <  1:93136/140‑1 (MQ=255)                                aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                                                                         <  1:527177/140‑1 (MQ=255)                                  gggCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCg                                                                                                       >  1:425233/1‑140 (MQ=255)                                     cTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                                                                                    <  1:908225/140‑1 (MQ=255)                                           gcgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCAGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGc                                                                                              <  1:672446/140‑1 (MQ=255)                                              ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATACCCAATGGGGAACAGGAACCCGTCCGGGAACAACAC‑CCCCCCCACAACTGCCGGGGGCAAAGGTCACAAATGCCGTTTTAAAGCGGCCCACAGCCgg                                                                                           >  2:203035/1‑139 (MQ=255)                                              ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                                           >  2:414053/1‑140 (MQ=255)                                              ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGATCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                                                                           >  2:596400/1‑140 (MQ=255)                                                      aTTGGCCCCCAGATGGTTGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGa                                                                                   <  1:203035/140‑1 (MQ=255)                                                        tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                                                                                 <  2:549214/140‑1 (MQ=255)                                                              ccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAAc                                                                           >  2:456802/1‑140 (MQ=255)                                                                aGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                                         <  1:187184/140‑1 (MQ=255)                                                                  aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                                         <  1:742022/138‑1 (MQ=255)                                                                  aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                                         >  2:742022/1‑138 (MQ=255)                                                                  aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGAt                                                                       <  1:452917/140‑1 (MQ=255)                                                                    ggTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCt                                                                     <  1:872911/140‑1 (MQ=255)                                                                            cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa                                                             >  1:96594/1‑140 (MQ=255)                                                                            cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa                                                             >  2:355100/1‑140 (MQ=255)                                                                              aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                                         <  1:414056/126‑1 (MQ=255)                                                                              aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                                                                         >  2:414056/1‑126 (MQ=255)                                                                                aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTc                                                          >  2:616746/1‑140 (MQ=255)                                                                                aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGTCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca                                                         >  2:618950/1‑140 (MQ=255)                                                                                aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca                                                         >  1:742926/1‑140 (MQ=255)                                                                                  cAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaa                                                       <  1:896862/140‑1 (MQ=255)                                                                                      aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaacaaa                                                   >  1:869834/1‑140 (MQ=255)                                                                                         aCCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAccc                                                >  2:673356/1‑140 (MQ=255)                                                                                         aCCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAccc                                                >  2:981151/1‑140 (MQ=255)                                                                                          ccAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaaca                                                     >  2:376159/1‑134 (MQ=255)                                                                                          ccAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaaca                                                     <  1:376159/134‑1 (MQ=255)                                                                                              tGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGt                                           >  2:428375/1‑140 (MQ=255)                                                                                                  gTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACg                                       >  2:779905/1‑140 (MQ=255)                                                                                                   tACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                                      >  1:598716/1‑140 (MQ=255)                                                                                                   tACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                                      >  1:105585/1‑140 (MQ=255)                                                                                                    aCAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGcc                                     >  2:850158/1‑140 (MQ=255)                                                                                                          aTCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGcc                                     <  1:345757/134‑1 (MQ=255)                                                                                                          aTCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGcc                                     >  2:345757/1‑134 (MQ=255)                                                                                                           tCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaac                              >  1:81318/1‑140 (MQ=255)                                                                                                           tCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaac                              >  2:779961/1‑140 (MQ=255)                                                                                                               ttCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaac                          >  1:315238/1‑140 (MQ=255)                                                                                                               ttCGGGAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaac                          >  1:388832/1‑140 (MQ=255)                                                                                                                  gggAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATTCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCTGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCg                       >  1:357460/1‑140 (MQ=255)                                                                                                                   ggAACAACAG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGc                      >  2:143773/1‑140 (MQ=255)                                                                                                                         acaG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGc                >  2:285039/1‑140 (MQ=255)                                                                                                                         acaG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGc                >  2:455761/1‑140 (MQ=255)                                                                                                                          caG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa               <  2:81318/140‑1 (MQ=255)                                                                                                                          caG‑CCCCCCCACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCa               <  2:96594/140‑1 (MQ=255)                                                                                                                            g‑CCCCCCCACAACTGGCGGGGGCGAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCaaa             >  2:501624/1‑140 (MQ=255)                                                                                                                               ccccccACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACATATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGt           >  1:867287/1‑140 (MQ=255)                                                                                                                                 ccccACAACTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGttt         <  1:926618/140‑1 (MQ=255)                                                                                                                                        aCTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAaca  >  2:201730/1‑140 (MQ=255)                                                                                                                                        aCTGGCGGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAaca  >  2:581000/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                      ATTTCGGGCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCCACAACTGGC‑GGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACA  >  NC_000913/3304683‑3304955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑