Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,257,110	(G)5→6	intergenic (+105/‑5)	dgkA → / → lexA	diacylglycerol kinase/DNA‑binding transcriptional repressor LexA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,257,105	1	.	G	100.0%	137.8 / NA	40	intergenic (+100/‑10)	dgkA/lexA	diacylglycerol kinase/DNA‑binding transcriptional repressor LexA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/20);  total (20/20)

ACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCA‑‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGC  >  NC_000913/4256970‑4257239                                                                                                                                         |                                                                                                                                        aCCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCGTAACTGTATATACACCCA‑‑gggg                                                                                                                                    >  1:696351/1‑140 (MQ=255)     ggTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCA‑‑ggggggcg                                                                                                                                <  2:696351/140‑4 (MQ=255)          aTTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATg                                                                                                                            <  2:170895/140‑1 (MQ=255)            tCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGaa                                                                                                                          >  2:951315/1‑140 (MQ=255)                   tGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTa                                                                                                                   <  2:484060/140‑1 (MQ=255)                   tGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTa                                                                                                                   <  1:126979/140‑1 (MQ=255)                    ggTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAGGGGGGGCGGAATGAAAGCGTTa                                                                                                                   >  2:167950/1‑140 (MQ=255)                        gCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGc                                                                                                              >  1:613712/1‑140 (MQ=255)                           ttttGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAg                                                                                                           <  1:629868/140‑1 (MQ=255)                                   aaCCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAag                                                                                                   <  1:423246/140‑1 (MQ=255)                                     cccTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAGGGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAaga                                                                                                  <  1:167950/140‑1 (MQ=255)                                     cccTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAagag                                                                                                 <  2:158455/140‑1 (MQ=255)                                          ccAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGc                                                                                                              >  1:664387/1‑122 (MQ=255)                                          ccAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGc                                                                                                              <  2:664387/122‑1 (MQ=255)                                           cAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGttt                                                                                           <  1:654591/140‑1 (MQ=255)                                                ttCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGAtct                                                                                      <  1:428512/140‑1 (MQ=255)                                                ttCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGAtct                                                                                      >  2:371767/1‑140 (MQ=255)                                                        aTCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTg                                                                              >  1:825301/1‑140 (MQ=255)                                                         tctcTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGa                                                                             <  2:824429/140‑1 (MQ=255)                                                                tttATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATc                                                                      <  2:467426/140‑1 (MQ=255)                                                                   aTTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGc                                                                   >  1:339651/1‑140 (MQ=255)                                                                         cAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGc                                                                   <  2:507125/134‑1 (MQ=255)                                                                         cAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGc                                                                   >  1:507125/1‑134 (MQ=255)                                                                            tttATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGt                                                          >  2:362481/1‑140 (MQ=255)                                                                                      cAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGa                                                >  2:548986/1‑140 (MQ=255)                                                                                      cAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGa                                                >  1:893222/1‑140 (MQ=255)                                                                                      cAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGa                                                <  2:893222/140‑1 (MQ=255)                                                                                        aaaTCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGAcg                                              <  1:362481/140‑1 (MQ=255)                                                                                        aaaTCGCCTTTTGCTGTATATACCCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGAcg                                              <  1:86055/140‑1 (MQ=255)                                                                                                             aCTCACAGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGt                         >  1:366191/1‑140 (MQ=255)                                                                                                                  caGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTggg                    >  2:804214/1‑140 (MQ=255)                                                                                                                  caGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTggg                    >  1:244320/1‑140 (MQ=255)                                                                                                                  caGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTggg                    >  2:375414/1‑140 (MQ=255)                                                                                                                   aGCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTgggg                   >  1:279760/1‑140 (MQ=255)                                                                                                                    gCATAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgt                  >  2:685000/1‑140 (MQ=255)                                                                                                                       tAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttcc               >  2:413446/1‑140 (MQ=255)                                                                                                                       tAACTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttcc               >  2:765141/1‑140 (MQ=255)                                                                                                                         aCTGTATATACACCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttccgt             <  2:366191/140‑1 (MQ=255)                                                                                                                                  acacCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAc    <  1:413446/140‑1 (MQ=255)                                                                                                                                  acacCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAc    <  1:765141/140‑1 (MQ=255)                                                                                                                                    acCCAG‑GGGGGCGGAATGAAAGCGTTGACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAcgc  >  2:980241/1‑140 (MQ=255)                                                                                                                                    acCCAG‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAcgc  <  1:804214/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ACCTGGTGCATTCTGTTATGGTCGCATTTTGGATAACCCTTCCAGAATTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAGCATAACTGTATATACACCCA‑‑GGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGC  >  NC_000913/4256970‑4257239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑