Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,157,814	(C)6→7	intergenic (+240/‑55)	ycfH → / → ptsG	putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,157,808	1	.	C	100.0%	161.8 / NA	47	intergenic (+234/‑61)	ycfH/ptsG	putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/21);  total (26/21)

TATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCT‑CCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCC  >  NC_000913/1157671‑1157939                                                                                                                                           |                                                                                                                                    tatGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCT‑cc                                                                                                                                   <  1:491472/140‑1 (MQ=255)   tGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCT‑cccc                                                                                                                                 <  1:813997/140‑1 (MQ=255)            aaaaTGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCa                                                                                                                         <  1:675881/140‑1 (MQ=255)                  tgCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCT‑cc                                                                                                                                   >  2:521772/1‑123 (MQ=255)                  tgCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCT‑cc                                                                                                                                   <  1:521772/123‑1 (MQ=255)                            ccAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTaaa                                                                                                         >  1:727985/1‑140 (MQ=255)                               aTGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTaaaaaa                                                                                                      <  1:655682/140‑1 (MQ=255)                               aTGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTaaaaaa                                                                                                      <  2:727985/140‑1 (MQ=255)                                 gATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAg                                                                                                    <  2:960710/140‑1 (MQ=255)                                 gATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAg                                                                                                    >  1:960710/1‑140 (MQ=255)                                 gATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAg                                                                                                    <  2:493578/140‑1 (MQ=255)                                  aTCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGc                                                                                                   >  2:833699/1‑140 (MQ=255)                                   tCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCa                                                                                                  >  1:922610/1‑140 (MQ=255)                                      gAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCAccc                                                                                               <  2:859190/140‑1 (MQ=255)                                          ttGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATAc                                                                                           >  2:323560/1‑140 (MQ=255)                                            gAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCAc                                                                                                 >  2:244418/1‑132 (MQ=255)                                            gAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCAc                                                                                                 <  1:244418/132‑1 (MQ=255)                                            gAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTc                                                                                         >  1:866276/1‑140 (MQ=255)                                               aCGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAgg                                                                                      >  2:88947/1‑140 (MQ=255)                                                 gTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAg                                                                                    <  2:866276/140‑1 (MQ=255)                                                  tGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGc                                                                                   >  1:245813/1‑140 (MQ=255)                                                   gATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCa                                                                                  >  2:708975/1‑140 (MQ=255)                                                          gTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCaa                                                                           <  1:323560/140‑1 (MQ=255)                                                           tCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAAt                                                                          >  2:654635/1‑140 (MQ=255)                                                                  aaTTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGttt                                                                   <  2:336011/140‑1 (MQ=255)                                                                      ggCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTACGTTTAAGa                                                               <  1:833699/140‑1 (MQ=255)                                                                                    ttACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAAc                                                 <  1:708975/140‑1 (MQ=255)                                                                                        tctGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGc                                             >  1:226336/1‑140 (MQ=255)                                                                                            tgtAATAAATAAAGGGCGCTTAGATGCCTTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCaaaa                                         >  1:787745/1‑140 (MQ=255)                                                                                                      aaaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTaaa                               >  1:59949/1‑140 (MQ=255)                                                                                                      aaaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTaaa                               >  2:502969/1‑140 (MQ=255)                                                                                                      aaaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTaaa                               >  2:305964/1‑140 (MQ=255)                                                                                                      aaaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTaaa                               >  1:736460/1‑140 (MQ=255)                                                                                                        aGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATc                             >  1:60294/1‑140 (MQ=255)                                                                                                        aGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATc                             >  2:316988/1‑140 (MQ=255)                                                                                                        aGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATc                             >  2:398201/1‑140 (MQ=255)                                                                                                              cTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGAt                       >  1:925605/1‑140 (MQ=255)                                                                                                               ttAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGAtg                      <  1:880825/140‑1 (MQ=255)                                                                                                                tAGATGCCCTGTACACGGCGAGGCTCTCCCCCCCTAGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGAtgc                     <  1:502969/140‑1 (MQ=255)                                                                                                                    tGCCCTGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGcc                 <  1:185329/140‑1 (MQ=255)                                                                                                                         tGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTAt            <  1:398201/140‑1 (MQ=255)                                                                                                                         tGTACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTAt            <  1:316988/140‑1 (MQ=255)                                                                                                                           tACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATcc          >  1:366703/1‑140 (MQ=255)                                                                                                                           tACACGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATcc          >  1:364674/1‑140 (MQ=255)                                                                                                                              acGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTa       <  2:366703/140‑1 (MQ=255)                                                                                                                              acGGCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTa       <  1:305964/140‑1 (MQ=255)                                                                                                                                ggCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTa       >  1:612161/1‑138 (MQ=255)                                                                                                                                ggCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTa       <  2:612161/138‑1 (MQ=255)                                                                                                                                ggCGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACt     >  1:889795/1‑140 (MQ=255)                                                                                                                                  cGAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGc   <  2:889795/140‑1 (MQ=255)                                                                                                                                  cGAGGCTCTCCCCCCCCTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGc   >  2:243134/1‑140 (MQ=255)                                                                                                                                   gAGGCTCTCCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGcc  >  1:678837/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                    TATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCT‑CCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCC  >  NC_000913/1157671‑1157939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑