Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,774,056 (G)5→6 coding (268/867 nt) ydiB → shikimate dehydrogenase/quinate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,774,0511.G100.0% 175.8 / NA 50V88G (GTG→GGG) ydiBshikimate dehydrogenase/quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/28);  total (22/28)

GTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑GGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTGC  >  NC_000913/1773918‑1774173
                                                                                                                                      |                                                                                                                          
gTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑gggggg                                                                                                                      <  2:497597/140‑2 (MQ=255)
 tGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑ggggggc                                                                                                                     >  2:694866/1‑138 (MQ=255)
  ggATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑ggggggcc                                                                                                                    <  1:201156/140‑4 (MQ=255)
  ggATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑ggggggcc                                                                                                                    >  1:752131/1‑137 (MQ=255)
       aCGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCaa                                                                                                                <  2:794367/140‑1 (MQ=255)
          aTAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAacac                                                                                                             <  2:891134/140‑1 (MQ=255)
           tAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACAcc                                                                                                            <  1:556793/140‑1 (MQ=255)
           tAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACAcc                                                                                                            <  1:589828/140‑1 (MQ=255)
            aGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCa                                                                                                           <  1:213673/140‑1 (MQ=255)
             gCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAGATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATGAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCAt                                                                                                          >  2:870516/1‑140 (MQ=255)
             gCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAac                                                                                                               >  1:808950/1‑135 (MQ=255)
             gCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAac                                                                                                               <  2:808950/135‑1 (MQ=255)
             gCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCAt                                                                                                          >  1:626992/1‑140 (MQ=255)
                 tCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGtt                                                                                                      >  1:872385/1‑140 (MQ=255)
                  ccTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTa                                                                                                     <  2:800835/140‑1 (MQ=255)
                  ccTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑ggggggcc                                                                                                                    <  1:876180/124‑4 (MQ=255)
                  ccTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑ggggggcc                                                                                                                    >  2:876180/1‑121 (MQ=255)
                      gAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAatga                                                                                                 <  2:924227/140‑1 (MQ=255)
                          aaTTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAatgatgat                                                                                             <  2:752131/140‑1 (MQ=255)
                                  gATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCt                                                                                     >  2:302566/1‑140 (MQ=255)
                                    ttAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCAATCAACACCATCGTTAATGATGATGGCTATCTGc                                                                                   <  2:872385/140‑1 (MQ=255)
                                      aaaaGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGt                                                                                 <  1:431255/140‑1 (MQ=255)
                                              tCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATaa                                                                         >  1:625051/1‑140 (MQ=255)
                                                    tGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGa                                                                   <  1:923799/140‑1 (MQ=255)
                                                         ggAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCa                                                              <  2:695932/140‑1 (MQ=255)
                                                                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGTCATATTc                                                  >  2:530221/1‑140 (MQ=255)
                                                                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTc                                                  >  2:771114/1‑140 (MQ=255)
                                                                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTc                                                  >  1:142966/1‑140 (MQ=255)
                                                                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTc                                                  >  1:362758/1‑140 (MQ=255)
                                                                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTc                                                  >  1:440393/1‑140 (MQ=255)
                                                                      cGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTcg                                                 <  1:109030/140‑1 (MQ=255)
                                                                      cGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTcg                                                 <  2:625051/140‑1 (MQ=255)
                                                                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTcgcg                                               >  2:957677/1‑140 (MQ=255)
                                                                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTcgcg                                               <  1:694866/140‑1 (MQ=255)
                                                                          gCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGcc                                             >  1:292744/1‑140 (MQ=255)
                                                                                caaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGTCATATTCGCGCCATTaaa                                       <  1:530221/140‑1 (MQ=255)
                                                                                  aacaacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAaga                                     <  1:616080/140‑1 (MQ=255)
                                                                                     aacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagagag                                  <  1:771114/140‑1 (MQ=255)
                                                                                             tgtgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTg                          >  2:766576/1‑140 (MQ=255)
                                                                                               tgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGat                        >  2:184740/1‑140 (MQ=255)
                                                                                               tgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGat                        >  2:344417/1‑140 (MQ=255)
                                                                                               tgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGat                        >  2:385677/1‑140 (MQ=255)
                                                                                               tgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGat                        >  2:799289/1‑140 (MQ=255)
                                                                                                    atGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCaa                   <  1:957677/140‑1 (MQ=255)
                                                                                                       ttGATGAATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAgg                >  1:242432/1‑140 (MQ=255)
                                                                                                         gatgaATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCa              >  1:583499/1‑140 (MQ=255)
                                                                                                         gatgaATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCa              >  2:489639/1‑140 (MQ=255)
                                                                                                            gaATTAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCaaaa           <  1:799289/140‑1 (MQ=255)
                                                                                                               ttAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGa        >  1:449025/1‑140 (MQ=255)
                                                                                                               ttAACACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGa        <  2:525175/140‑1 (MQ=255)
                                                                                                                 aaCACCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATg      <  1:766576/140‑1 (MQ=255)
                                                                                                                    cccAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTg   <  2:292744/139‑1 (MQ=255)
                                                                                                                    cccAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTg   <  1:961136/139‑1 (MQ=255)
                                                                                                                    acCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTg   <  1:385677/140‑1 (MQ=255)
                                                                                                                    acCAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTg   <  1:304516/140‑1 (MQ=255)
                                                                                                                     ccAGCTGCCAAACTGGTGGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTGc  <  1:489639/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                          
GTGGATAACGATAGCTTTCCTGGAGCAATTGAAGGATTAAAAGCCCTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGT‑GGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTGATATCAAAGGCAAAACGATGGTGC  >  NC_000913/1773918‑1774173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑