Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,267,780	(T)6→7	intergenic (+69/‑49)	yeiQ → / → yeiR	putative dehydrogenase, NAD‑dependent/zinc‑binding GTPase YeiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,774	1	.	T	100.0%	102.1 / NA	30	intergenic (+63/‑55)	yeiQ/yeiR	putative dehydrogenase, NAD‑dependent/zinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (14/16);  total (14/16)

CATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑TTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTT  >  NC_000913/2267639‑2267896                                                                                                                                         |                                                                                                                           cATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑tttt                                                                                                                        >  1:301518/1‑140 (MQ=255)  aTCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑ttttt                                                                                                                       <  2:301518/140‑1 (MQ=255)    cGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTCAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑ttttttt                                                                                                                     >  1:675092/1‑139 (MQ=255)                gCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTaa                                                                                                          >  2:747258/1‑140 (MQ=255)                gCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑ttttt                                                                                                                       >  1:936515/1‑126 (MQ=255)                gCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑ttttt                                                                                                                       <  2:936515/126‑1 (MQ=255)                   acaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGc                                                                                                       <  2:674548/140‑1 (MQ=255)                      aaTCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCa                                                                                                    <  1:597598/140‑1 (MQ=255)                             cAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACt                                                                                             <  2:732021/140‑1 (MQ=255)                                ttCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTAc                                                                                          <  2:974072/140‑1 (MQ=255)                                  cGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCa                                                                                                    >  2:390701/1‑128 (MQ=255)                                  cGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCa                                                                                                    <  1:390701/128‑1 (MQ=255)                                          aTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTTAGTTAACTTACCAGAGTATTg                                                                                >  1:608119/1‑140 (MQ=255)                                               gcgcTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAg                                                                           <  1:747258/140‑1 (MQ=255)                                                         acacCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGAc                                                                 <  2:4144/140‑1 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      <  2:272221/131‑1 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      >  2:139680/1‑131 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      <  1:139680/131‑1 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      >  1:272221/1‑131 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGAc                                                                 >  1:562644/1‑136 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGAc                                                                 <  2:562644/136‑1 (MQ=255)                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAgg                                                             >  1:130455/1‑140 (MQ=255)                                                               tCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      >  1:48644/1‑129 (MQ=255)                                                               tCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      <  2:48644/129‑1 (MQ=255)                                                               tCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGAc                                                           >  2:347240/1‑140 (MQ=255)                                                               tCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGAc                                                           >  1:474833/1‑140 (MQ=255)                                                                             tttCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCg                                             <  1:347240/140‑1 (MQ=255)                                                                                cTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGtt                                          >  1:334975/1‑140 (MQ=255)                                                                                        aaaGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGc                                  >  2:60883/1‑140 (MQ=255)                                                                                                tGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaa                          >  1:57985/1‑140 (MQ=255)                                                                                                tGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaa                          >  1:37079/1‑140 (MQ=255)                                                                                                           ttCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGa               <  2:924474/140‑1 (MQ=255)                                                                                                                 cGCTCTCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTTAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttcttc         <  2:608119/140‑1 (MQ=255)                                                                                                                   ctctCCCCTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCAt       <  2:434229/140‑1 (MQ=255)                                                                                                                        cccTTCTCTTTTCTGCCTTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGtt  <  2:780824/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCC‑TTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTT  >  NC_000913/2267639‑2267896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑