Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,866	A→G	R523R (CGT→CGC)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,866	0	A	G	100.0%	195.2 / NA	55	R523R (CGT→CGC)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/30);  total (25/30)

CGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCT  >  NC_000913/2966729‑2966999                                                                                                                                          |                                                                                                                                      cgTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCcgcg                                                                                                                                     >  2:875080/1‑140 (MQ=255) cgTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCcgcg                                                                                                                                     >  2:271851/1‑140 (MQ=255)  gTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCgcgc                                                                                                                                    <  2:167224/140‑1 (MQ=255)   tGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGcc                                                                                                                                   >  2:595665/1‑140 (MQ=255)       tcatcaACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAAcc                                                                                                                               <  2:445496/140‑1 (MQ=255)        catcaACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAAccc                                                                                                                              <  2:236640/140‑1 (MQ=255)              cTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCAt                                                                                                                        <  2:295242/140‑1 (MQ=255)                  aTCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGAt                                                                                                                    >  2:950539/1‑140 (MQ=255)                  aTCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGAt                                                                                                                    >  2:321483/1‑140 (MQ=255)                  aTCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCACCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGAt                                                                                                                    >  1:392860/1‑140 (MQ=255)                              gACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGa                                                                                                        <  2:809431/140‑1 (MQ=255)                              gACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGa                                                                                                        <  1:950539/140‑1 (MQ=255)                               aCCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGAt                                                                                                       <  1:375080/140‑1 (MQ=255)                                 cATCGGCAACAGAATATTCAGGTTACCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCg                                                                                                     <  1:926268/140‑1 (MQ=255)                                  aTCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCgg                                                                                                    >  1:712654/1‑140 (MQ=255)                                     ggCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCAt                                                                                                 >  2:142874/1‑140 (MQ=255)                                        aaCAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTa                                                                                              <  2:877080/140‑1 (MQ=255)                                         aCAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTaa                                                                                             <  2:237593/140‑1 (MQ=255)                                            gAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGc                                                                                          <  2:401151/140‑1 (MQ=255)                                            gAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGc                                                                                          <  2:712654/140‑1 (MQ=255)                                              atatTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAg                                                                                        <  2:762245/140‑1 (MQ=255)                                                  tCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAgctgc                                                                                    >  1:649181/1‑140 (MQ=255)                                                       ttgaccGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAgc                                                                                       >  1:804183/5‑132 (MQ=255)                                                       ttGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCC‑GGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCt                                                                              >  2:597256/1‑140 (MQ=255)                                                       ttGACCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAgc                                                                                       <  2:804183/132‑1 (MQ=255)                                                         gCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTg                                                                             >  2:222650/1‑140 (MQ=255)                                                          cccGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGc                                                                            >  2:113855/1‑140 (MQ=255)                                                          cccGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCC‑GGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCt                                                                           <  1:597256/140‑1 (MQ=255)                                                           ccGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCt                                                                           >  1:785433/1‑140 (MQ=255)                                                              tAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCg                                                                        >  2:898403/1‑140 (MQ=255)                                                                gCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGAc                                                                      <  2:392860/140‑1 (MQ=255)                                                                   gCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGATGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATc                                                                   <  1:699944/140‑1 (MQ=255)                                                                        aGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAgtg                                                              >  2:676020/1‑138 (MQ=255)                                                                          cACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTa                                                            <  2:180559/140‑1 (MQ=255)                                                                          cACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTa                                                            <  2:811638/140‑1 (MQ=255)                                                                              cagcaTCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCa                                                        >  1:281833/1‑140 (MQ=255)                                                                                   tCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTg                                                   <  1:875080/140‑1 (MQ=255)                                                                                   tCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTg                                                   <  2:785433/140‑1 (MQ=255)                                                                                    cGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGa                                                  >  2:16318/1‑140 (MQ=255)                                                                                    cGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGa                                                  <  1:142874/140‑1 (MQ=255)                                                                                      cccGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACg                                                >  2:473470/1‑140 (MQ=255)                                                                                                 aTCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCAtt                                     >  2:115837/1‑140 (MQ=255)                                                                                                     agaagaTCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGG‑ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACa                                >  1:220931/1‑140 (MQ=255)                                                                                                         gaTCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACAttt                             >  1:44836/1‑140 (MQ=255)                                                                                                          aTCTCCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTg                            >  2:809191/1‑140 (MQ=255)                                                                                                             tcCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTgcag                         <  2:649181/140‑1 (MQ=255)                                                                                                             tcCGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTgcag                         <  2:281833/140‑1 (MQ=255)                                                                                                              ccGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTgcagc                        <  2:44836/140‑1 (MQ=255)                                                                                                               cGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTgcagca                       <  1:222650/140‑1 (MQ=255)                                                                                                               cGGCTGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTgcagca                       <  1:113855/140‑1 (MQ=255)                                                                                                                   tGATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATccc                   <  1:271851/140‑1 (MQ=255)                                                                                                                    gATCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATcccc                  >  2:838256/1‑140 (MQ=255)                                                                                                                     aTCGAGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCt                 >  1:215600/1‑140 (MQ=255)                                                                                                                         aGCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATa             <  2:654972/140‑1 (MQ=255)                                                                                                                          gCGTAATGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATAc            <  1:16318/140‑1 (MQ=255)                                                                                                                               aTGCGAATCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTgcgc       >  1:464169/1‑140 (MQ=255)                                                                                                                                    aaTCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCt  >  1:924172/1‑140 (MQ=255)                                                                                                                                     aTCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTg          >  2:292760/1‑131 (MQ=255)                                                                                                                                     aTCCCGCGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTg          <  1:292760/131‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCT  >  NC_000913/2966729‑2966999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑