Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,592	T→C	C511C (TGT→TGC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,592	0	T	C	100.0%	73.9 / NA	22	C511C (TGT→TGC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/0);  total (22/0)

GATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATT  >  NC_000913/3017464‑3017727                                                                                                                                 |                                                                                                                                        gATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGa                                                                                                                              >  1:257107/1‑140 (MQ=255)           aaTACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGt                                                                                                                    >  2:790213/1‑140 (MQ=255)                     tCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAata                                                                                                          >  1:858101/1‑140 (MQ=255)                       tGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATc                                                                                                        >  1:860051/1‑140 (MQ=255)                       tGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATc                                                                                                        >  1:455157/1‑140 (MQ=255)                                                cgcgCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTgg                                                                               >  2:773602/1‑140 (MQ=255)                                                                 aCCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGaa                                                              >  2:800323/1‑140 (MQ=255)                                                                       gcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAg                                                        >  2:6791/1‑140 (MQ=255)                                                                           cgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAAc                                                    >  1:147476/1‑140 (MQ=255)                                                                             ctccccccGCCATTCCCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGc                                                  <  2:147476/135‑1 (MQ=255)                                                                                 gCCCGCCATTACCGGTCATGTTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGc                                              >  1:208024/1‑140 (MQ=255)                                                                                 gCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGc                                              >  2:789883/1‑140 (MQ=255)                                                                                      ccATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACaaa                                         >  2:763019/1‑140 (MQ=255)                                                                                       cATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAAc                                        >  1:565233/1‑140 (MQ=255)                                                                                           cccGGTCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGc                                    <  1:259828/139‑1 (MQ=255)                                                                                                tCATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGtt                               <  1:800323/140‑1 (MQ=255)                                                                                                 cATATTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                              >  1:741883/1‑140 (MQ=255)                                                                                                   tatTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACaaa                                         >  1:841623/1‑127 (MQ=255)                                                                                                   tatTTGCGATCACCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACaaa                                         <  2:841623/127‑1 (MQ=255)                                                                                                        gCGATCCCCAGTCCAAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCa                       <  2:257107/140‑1 (MQ=255)                                                                                                              aCCAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCAt                 >  1:170295/1‑140 (MQ=255)                                                                                                                cAGTGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATcc               >  2:99283/1‑140 (MQ=255)                                                                                                                 ggTGCAAAGACAGGTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCg              <  2:208024/139‑1 (MQ=255)                                                                                                                   tGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGt            >  1:822508/1‑140 (MQ=255)                                                                                                                   tGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGt            <  1:790213/140‑1 (MQ=255)                                                                                                                   tGCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGt            <  1:763019/140‑1 (MQ=255)                                                                                                                    gCCAATACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGtt           >  2:152452/1‑140 (MQ=255)                                                                                                                       aaTACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGcc        <  1:520929/140‑1 (MQ=255)                                                                                                                       aaTACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGcc        <  1:390396/140‑1 (MQ=255)                                                                                                                        aTACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCg       >  1:291372/1‑140 (MQ=255)                                                                                                                         tACAACTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGt      >  1:432963/1‑140 (MQ=255)                                                                                                                             aCTGCACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAtt  >  2:519800/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        GATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATT  >  NC_000913/3017464‑3017727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑