Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,506	A→G	I386V (ATC→GTC)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,506	0	A	G	100.0%	156.3 / NA	46	I386V (ATC→GTC)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

GGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAA  >  NC_000913/3025369‑3025639                                                                                                                                          |                                                                                                                                      ggCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGcgtc                                                                                                                                     >  2:544781/1‑140 (MQ=255)      aCCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGcgtcatcat                                                                                                                                >  2:271033/1‑140 (MQ=255)       ccATCGCGGTAGTGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCgtcatcatc                                                                                                                               <  1:873938/140‑1 (MQ=255)           cgcgGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCa                                                                                                                           >  2:954024/1‑140 (MQ=255)                tAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGt                                                                                                                      >  1:214959/1‑140 (MQ=255)                     cTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTaaa                                                                                                                 >  1:77314/1‑140 (MQ=255)                     cTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTaaa                                                                                                                 >  1:507604/1‑140 (MQ=255)                             ccTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTg                                                                                                         >  1:887486/1‑140 (MQ=255)                                  gCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGt                                                                                                                      <  2:737199/122‑1 (MQ=255)                                  gCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGt                                                                                                                      >  1:737199/1‑122 (MQ=255)                                      aTTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTa                                                                                                >  2:954044/1‑140 (MQ=255)                                      aTTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGGTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGCTTAAAGCGCCGTGAAACACTTa                                                                                                >  2:203442/1‑140 (MQ=255)                                             atgatTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGc                                                                                         <  1:544781/140‑1 (MQ=255)                                             atgatTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGc                                                                                         >  1:411779/1‑140 (MQ=255)                                             atgatTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGc                                                                                         >  1:730189/1‑140 (MQ=255)                                              tgatTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGcc                                                                                        <  1:271033/140‑1 (MQ=255)                                                atTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCAc                                                                                      >  2:360082/1‑140 (MQ=255)                                                      ggcTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCttt                                                                                <  1:131881/140‑1 (MQ=255)                                                         ttcttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCtttagg                                                                             >  1:609532/1‑140 (MQ=255)                                                          tcttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCtttaggt                                                                            <  1:954024/140‑1 (MQ=255)                                                           cttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCActtctt                                                                                 <  1:611956/134‑1 (MQ=255)                                                           cttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCActtctt                                                                                 >  2:611956/1‑134 (MQ=255)                                                                 gACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGc                                                                     <  1:786824/140‑1 (MQ=255)                                                                     aTTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGTTTgg                                                                 <  2:609532/140‑1 (MQ=255)                                                                      ttCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGc                                                                >  2:774397/1‑140 (MQ=255)                                                                       tCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCg                                                               <  1:866131/140‑1 (MQ=255)                                                                       tCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCACCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCg                                                               <  2:865733/140‑1 (MQ=255)                                                                             ggCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTTAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCt                                                         >  1:393402/1‑140 (MQ=255)                                                                                 gTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGa                                                     >  1:479184/1‑140 (MQ=255)                                                                                  ttCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGGAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGAt                                                    >  2:383377/1‑140 (MQ=255)                                                                                   tCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATc                                                   >  1:542690/1‑140 (MQ=255)                                                                                     tGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATccc                                                 >  1:686899/1‑140 (MQ=255)                                                                                              cAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATCGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAAttttt                                        <  1:219891/140‑1 (MQ=255)                                                                                                          tGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGGAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCa                            <  1:383377/140‑1 (MQ=255)                                                                                                          tGTTTTCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACGCTTATTGCCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCa                            <  1:203442/140‑1 (MQ=255)                                                                                                            ttttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGc                          <  2:365576/140‑1 (MQ=255)                                                                                                            ttttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGc                          <  2:887486/140‑1 (MQ=255)                                                                                                            ttttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAACATTGCCAGc                          <  1:774397/140‑1 (MQ=255)                                                                                                             tttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGcc                         <  2:937072/140‑1 (MQ=255)                                                                                                              ttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCct                        <  2:411779/140‑1 (MQ=255)                                                                                                              ttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCct                        <  2:487034/140‑1 (MQ=255)                                                                                                              ttCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCct                        <  1:954044/140‑1 (MQ=255)                                                                                                               tCCATGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCctc                       <  2:77314/140‑1 (MQ=255)                                                                                                                   tGATTGCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTAtt                   >  1:173994/1‑140 (MQ=255)                                                                                                                        gCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGc                          <  2:156340/128‑1 (MQ=255)                                                                                                                        gCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGc                          >  1:156340/1‑128 (MQ=255)                                                                                                                        gCCATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGt              >  1:711376/1‑140 (MQ=255)                                                                                                                         ccATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGc                              >  1:597793/1‑123 (MQ=255)                                                                                                                         ccATCGCAGGGATTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGc                              <  2:597793/123‑1 (MQ=255)                                                                                                                                    aTTCGCGTCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGaaaa  <  2:686899/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAA  >  NC_000913/3025369‑3025639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑