Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,165,926	G→A	N16N (AAC→AAT)	parC ←	DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,165,926	0	G	A	100.0%	273.2 / NA	77	N16N (AAC→AAT)	parC	DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/32);  total (45/32)

TTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCGTTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCAC  >  NC_000913/3165790‑3166057                                                                                                                                         |                                                                                                                                    ttttAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCAttt                                                                                                                                  <  2:672806/140‑1 (MQ=255)     aaaTTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCg                                                                                                                              <  2:542518/140‑1 (MQ=255)     aaaTTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCg                                                                                                                              <  2:847439/140‑1 (MQ=255)                      tCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCa                                                                                                             >  2:206196/1‑140 (MQ=255)                      tCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCa                                                                                                             <  1:206196/140‑1 (MQ=255)                           cccAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGgcg                                                                                                        <  1:159354/140‑1 (MQ=255)                             cAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGgcg                                                                                                        <  1:885684/138‑1 (MQ=255)                             cAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGgcg                                                                                                        >  2:885684/1‑138 (MQ=255)                             cAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGct                                                                                                      <  2:230976/140‑1 (MQ=255)                               gTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGctct                                                                                                    >  1:629898/1‑140 (MQ=255)                                    gACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCat                                                                                               >  1:713839/1‑140 (MQ=255)                                          gCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTCCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCt                                                                                         >  1:54659/1‑140 (MQ=255)                                           cATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTc                                                                                        <  2:206848/140‑1 (MQ=255)                                            aTACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCa                                                                                       <  1:846717/140‑1 (MQ=255)                                                    tGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCt                                                                               >  2:331732/1‑140 (MQ=255)                                                    tGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCt                                                                               >  1:946952/1‑140 (MQ=255)                                                    tGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCt                                                                               >  2:448667/1‑140 (MQ=255)                                                              aaCAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTg                                                                              <  2:764317/131‑1 (MQ=255)                                                              aaCAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTg                                                                              >  1:764317/1‑131 (MQ=255)                                                              aaCAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCaa                                                                     >  1:467753/1‑140 (MQ=255)                                                              aaCAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCaa                                                                     >  2:515098/1‑140 (MQ=255)                                                                 aGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACtt                                                                  <  1:420024/140‑1 (MQ=255)                                                                     ttCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTc                                                              >  2:690069/1‑140 (MQ=255)                                                                        aGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGcc                                                           >  2:903289/1‑140 (MQ=255)                                                                        aGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGcc                                                           >  2:697619/1‑140 (MQ=255)                                                                          aCCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAg                                                         <  1:697619/140‑1 (MQ=255)                                                                             aTCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCt                                                      >  2:894928/1‑140 (MQ=255)                                                                             aTCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCt                                                      >  2:567267/1‑140 (MQ=255)                                                                             aTCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCt                                                      >  1:25283/1‑140 (MQ=255)                                                                             aTCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCt                                                      >  1:261835/1‑140 (MQ=255)                                                                              tCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCtt                                                     >  1:692012/1‑140 (MQ=255)                                                                               cACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTc                                                    >  2:232941/1‑140 (MQ=255)                                                                                aCCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCa                                                   <  1:232941/140‑1 (MQ=255)                                                                                aCCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGACGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCa                                                   <  1:262239/140‑1 (MQ=255)                                                                                 ccAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCaa                                                  <  1:841541/140‑1 (MQ=255)                                                                                       aaCGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGc                                            <  2:261835/140‑1 (MQ=255)                                                                                         cGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCaa                                          >  2:735416/1‑140 (MQ=255)                                                                                            cAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAatat                                       >  2:262725/1‑140 (MQ=255)                                                                                            cAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAatat                                       >  1:499965/1‑140 (MQ=255)                                                                                               cGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGc                                    >  2:890023/1‑140 (MQ=255)                                                                                                gCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGcc                                   <  2:692012/140‑1 (MQ=255)                                                                                                  aCGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCgg                                 <  2:964808/140‑1 (MQ=255)                                                                                                  aCGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCgg                                 <  1:890023/140‑1 (MQ=255)                                                                                                  aCGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCgg                                 <  1:735416/140‑1 (MQ=255)                                                                                                  aCGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTCCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCgg                                 <  2:54659/140‑1 (MQ=255)                                                                                                   cGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGga                                >  2:403061/1‑140 (MQ=255)                                                                                                     gTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGgaga                              <  1:690069/140‑1 (MQ=255)                                                                                                      tCCATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGAt                             <  1:262725/140‑1 (MQ=255)                                                                                                       ccATGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGAta                            <  1:515098/140‑1 (MQ=255)                                                                                                         aTGATCACGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGAtact                          <  1:331732/140‑1 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCgg                                 <  2:295249/126‑1 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCgg                                 >  1:295249/1‑126 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCg                                  <  1:809118/125‑1 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCg                                  >  2:809118/1‑125 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  2:769997/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  2:956144/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  1:133127/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  2:551170/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  2:21774/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  2:176463/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  1:427807/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  2:107037/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  1:900882/1‑140 (MQ=255)                                                                                                                cGTACATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTAt                   >  1:584832/1‑140 (MQ=255)                                                                                                                    cATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGc               <  1:176463/140‑1 (MQ=255)                                                                                                                    cATGGAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGc               >  2:741650/1‑140 (MQ=255)                                                                                                                       ggAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCcgc            >  2:932778/1‑140 (MQ=255)                                                                                                                       ggAGTAGTTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCcgc            >  1:44120/1‑140 (MQ=255)                                                                                                                         agtagtTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGt          >  1:107335/1‑140 (MQ=255)                                                                                                                         agtagtTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGt          >  1:517235/1‑140 (MQ=255)                                                                                                                         agtagtTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGt          <  2:584832/140‑1 (MQ=255)                                                                                                                           tagtTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCg        <  1:448667/140‑1 (MQ=255)                                                                                                                           tagtTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCg        >  1:174283/1‑140 (MQ=255)                                                                                                                             gtTTAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAg      <  2:517235/140‑1 (MQ=255)                                                                                                                              tttAAGTAGGCATTTTCCTTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGt     <  2:80040/140‑1 (MQ=255)                                                                                                                              tttAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGt     >  2:175873/1‑140 (MQ=255)                                                                                                                               ttAAGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTc    >  1:241035/1‑140 (MQ=255)                                                                                                                                 aaGTAGGCATTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTcac  <  2:499965/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCGTTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCCAGCCTTCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCAC  >  NC_000913/3165790‑3166057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑