Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,229,057	A→T	D57V (GAT→GTT)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,229,057	0	A	T	97.9%	161.7 / ‑3.6	48	D57V (GAT→GTT)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (25/22);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01

GCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGG  >  NC_000913/3228922‑3229187                                                                                                                                        |                                                                                                                                   gcgcTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTtctc                                                                                                                                >  2:616418/1‑140 (MQ=255)    ctctGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGa                                                                                                                             <  1:616418/140‑1 (MQ=255)     tctGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAg                                                                                                                            <  1:395277/140‑1 (MQ=255)      ctgctgATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGc                                                                                                                           <  1:133426/140‑1 (MQ=255)       tgctgATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGcc                                                                                                                          >  2:840435/1‑140 (MQ=255)         ctgATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTg                                                                                                                        >  1:233770/1‑140 (MQ=255)         ctgATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTg                                                                                                                        >  1:486405/1‑140 (MQ=255)            aTAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCa                                                                                                                     >  1:628378/1‑140 (MQ=255)            aTAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCa                                                                                                                     <  2:558634/140‑1 (MQ=255)                gTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGt                                                                                                                 <  1:840435/140‑1 (MQ=255)                gTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGt                                                                                                                 <  1:220400/140‑1 (MQ=255)                gTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGt                                                                                                                 <  1:73383/140‑1 (MQ=255)                 tttttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTg                                                                                                                        <  2:70225/132‑1 (MQ=255)                 tttttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTg                                                                                                                        >  1:70225/1‑132 (MQ=255)                   tttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCAt                                                                                                              <  2:486405/140‑1 (MQ=255)                    ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATc                                                                                                             <  2:482845/140‑1 (MQ=255)                               ccGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGAc                                                                                                  <  1:54545/140‑1 (MQ=255)                                 gCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACgg                                                                                                <  1:408278/140‑1 (MQ=255)                                      cGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTa                                                                                           >  1:959793/1‑140 (MQ=255)                                      cGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTa                                                                                           >  1:601697/1‑140 (MQ=255)                                                                   gTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTgcg                                                              <  2:566792/140‑1 (MQ=255)                                                                        ggCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCgctgct                                                         <  1:158121/140‑1 (MQ=255)                                                                         gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCTTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGctgctg                                                        >  2:306321/1‑140 (MQ=255)                                                                          cgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGa                                                       >  1:348415/1‑140 (MQ=255)                                                                            cgccgcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACg                                                     >  1:121501/1‑140 (MQ=255)                                                                              ccgcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGa                                                   >  2:787045/1‑140 (MQ=255)                                                                                  aGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAagag                                               <  2:628378/140‑1 (MQ=255)                                                                                    tACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGGTGCGCTGCTGACGGAAGAGTa                                             >  2:706179/1‑140 (MQ=255)                                                                                       aTGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACAt                                          <  2:233770/140‑1 (MQ=255)                                                                                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAag                                                 >  1:853969/1‑126 (MQ=255)                                                                                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAag                                                 <  2:853969/126‑1 (MQ=255)                                                                                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACttt                                   >  2:303963/1‑140 (MQ=255)                                                                                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACttt                                   >  1:779184/1‑140 (MQ=255)                                                                                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACttt                                   >  1:769786/1‑140 (MQ=255)                                                                                                tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTAt                                 >  2:973509/1‑140 (MQ=255)                                                                                                tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTAt                                 >  2:269375/1‑140 (MQ=255)                                                                                                tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTAt                                 >  1:937116/1‑140 (MQ=255)                                                                                                     ttacgaCGATCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCa                            <  1:303963/140‑1 (MQ=255)                                                                                                        cgacgaTCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCa                         >  2:747714/1‑140 (MQ=255)                                                                                                        cgacgaTCACCAGCGTTTTAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCa                         >  1:765586/1‑140 (MQ=255)                                                                                                                       ttttAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGa          <  2:370832/140‑1 (MQ=255)                                                                                                                       ttttAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGa          >  2:955534/1‑140 (MQ=255)                                                                                                                        tttAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGAc         <  2:601697/140‑1 (MQ=255)                                                                                                                        tttAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGAc         <  2:959793/140‑1 (MQ=255)                                                                                                                         ttAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGAcc        >  1:95599/1‑140 (MQ=255)                                                                                                                          tAATCCGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCg       <  1:269375/140‑1 (MQ=255)                                                                                                                              ccGTTTTTCGTTCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTg   >  1:820272/1‑140 (MQ=255)                                                                                                                               cGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTgg  >  2:964642/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGG  >  NC_000913/3228922‑3229187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑