Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,281,767	T→C	intergenic (+142/‑209)	agaA → / → agaS	putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,281,767	0	T	C	100.0%	158.9 / NA	45	intergenic (+142/‑209)	agaA/agaS	putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/19);  total (26/19)

CTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGAC  >  NC_000913/3281633‑3281896                                                                                                                                       |                                                                                                                                  cTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTgcg                                                                                                                              >  2:332024/1‑140 (MQ=255) cTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTgcg                                                                                                                              <  1:913192/140‑1 (MQ=255)      ttATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCa                                                                                                                         <  1:861716/140‑1 (MQ=255)      ttATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCa                                                                                                                         <  2:444371/140‑1 (MQ=255)      ttATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCa                                                                                                                         <  2:706424/140‑1 (MQ=255)        aTTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATc                                                                                                                       <  2:279257/140‑1 (MQ=255)            ggCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGc                                                                                                                   >  1:534124/1‑140 (MQ=255)            ggCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGc                                                                                                                   >  1:618598/1‑140 (MQ=255)             gCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCa                                                                                                                  <  1:605732/140‑1 (MQ=255)              ccTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCaa                                                                                                                 <  2:618598/140‑1 (MQ=255)                        ccccGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGc                                                                                                       >  2:857882/1‑140 (MQ=255)                                   ctttctttTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTAtct                                                                                            >  1:877883/1‑140 (MQ=255)                                       ctttttctttCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTAtcttttc                                                                                        >  2:422561/1‑140 (MQ=255)                                                          cTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAAttt                                                                     <  1:332024/140‑1 (MQ=255)                                                            gtgCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCg                                                                   >  2:918215/1‑140 (MQ=255)                                                            gtgCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCg                                                                   >  2:900935/1‑140 (MQ=255)                                                             tgCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGt                                                                  >  2:510177/1‑140 (MQ=255)                                                              gCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGtt                                                                 <  1:510177/140‑1 (MQ=255)                                                                  cGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTAt                                                             >  1:521850/1‑140 (MQ=255)                                                                    gtgtCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGTGCCGCATCCAGCAATCCCTTTTGCGTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTAttt                                                           >  2:913745/1‑140 (MQ=255)                                                                     tgtCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTc                                                          >  1:855427/1‑140 (MQ=255)                                                                       tCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGt                                                                  <  2:464454/130‑1 (MQ=255)                                                                       tCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGt                                                                  >  1:464454/1‑130 (MQ=255)                                                                       tCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                                                        >  1:867703/1‑140 (MQ=255)                                                                       tCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                                                        >  2:171745/1‑140 (MQ=255)                                                                       tCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                                                        >  1:500005/1‑140 (MQ=255)                                                                       tCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                                                        >  2:462265/1‑140 (MQ=255)                                                                          gATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCttttt                                                     <  2:521850/140‑1 (MQ=255)                                                                           aTGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtttttt                                                    <  1:918215/140‑1 (MQ=255)                                                                                 cGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCAt                                              >  2:188465/1‑140 (MQ=255)                                                                                     aaCGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGaa                                          <  2:589091/140‑1 (MQ=255)                                                                                             ttATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGt                                  <  2:534124/140‑1 (MQ=255)                                                                                             ttATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGt                                  >  2:107147/1‑140 (MQ=255)                                                                                               aTCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGttt                                >  2:41998/1‑140 (MQ=255)                                                                                               aTCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGttt                                <  1:202175/140‑1 (MQ=255)                                                                                                         cAGTTGGTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCtata                      >  1:713900/1‑140 (MQ=255)                                                                                                              ggTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGAttt                 >  2:443368/1‑140 (MQ=255)                                                                                                              ggTGACCGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGAttt                 >  2:42311/1‑140 (MQ=255)                                                                                                                    cGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCa           >  1:616408/1‑140 (MQ=255)                                                                                                                    cGCAAGGCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCa           >  1:226660/1‑140 (MQ=255)                                                                                                                         ggCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGaa      >  2:463149/1‑140 (MQ=255)                                                                                                                         ggCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGaa      <  2:226660/140‑1 (MQ=255)                                                                                                                         ggCCGGATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGaa      <  1:42311/140‑1 (MQ=255)                                                                                                                             ggATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGAc  <  1:41998/140‑1 (MQ=255)                                                                                                                             ggATAAAGCGCTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTCTATTTCTTTTTTCTCCACTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGAc  <  2:877883/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  CTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGAC  >  NC_000913/3281633‑3281896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑