Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	513,158	T→C	F195L (TTC→CTC)	ybaT →	putative transporter YbaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	513,158	0	T	C	100.0%	159.8 / NA	46	F195L (TTC→CTC)	ybaT	putative transporter YbaT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

CCATGCGGTAGGGCGGCTGGAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTACCACGTTGCT  >  NC_000913/513025‑513294                                                                                                                                      |                                                                                                                                         ccATGCGGTAGGGCGGCTGGAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCtcttct                                                                                                                                    >  1:380075/1‑140 (MQ=255)                    gAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACttt                                                                                                                 <  2:786297/140‑1 (MQ=255)                         gATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTg                                                                                                            <  2:917427/140‑1 (MQ=255)                          aTCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGc                                                                                                           >  1:544966/1‑140 (MQ=255)                              tcgtcgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTAt                                                                                                       >  1:876140/1‑140 (MQ=255)                               cgtcgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTc                                                                                                                >  1:594300/1‑130 (MQ=255)                               cgtcgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTc                                                                                                                <  2:594300/130‑1 (MQ=255)                                 tcgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCg                                                                                                    >  1:344511/1‑140 (MQ=255)                                 tcgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCg                                                                                                    <  2:424139/140‑1 (MQ=255)                                 tcgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCg                                                                                                    <  2:344511/140‑1 (MQ=255)                                  cgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGc                                                                                                     <  1:366922/138‑1 (MQ=255)                                  cgGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGc                                                                                                     >  2:366922/1‑138 (MQ=255)                                     cATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTGCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCt                                                                                                >  2:826028/1‑140 (MQ=255)                                               atgatCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCatgat                                                                                      >  2:759324/1‑140 (MQ=255)                                               atgatCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCatgat                                                                                      >  1:622087/1‑140 (MQ=255)                                               atgatCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCatgat                                                                                      >  2:558598/1‑140 (MQ=255)                                                             gccgATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCCCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGcggcgg                                                                        <  2:937740/137‑1 (MQ=255)                                                             gCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGcggcgg                                                                        >  2:130966/1‑140 (MQ=255)                                                             gCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGcggcgg                                                                        >  1:807961/1‑140 (MQ=255)                                                             gCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGcggcgg                                                                        >  1:717342/1‑140 (MQ=255)                                                                 attattGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGAt                                                                      >  1:928262/1‑138 (MQ=255)                                                                 attattGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGAt                                                                      <  2:928262/138‑1 (MQ=255)                                                                  ttattGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATaaa                                                                   >  2:635280/1‑140 (MQ=255)                                                                  ttattGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATaaa                                                                   >  2:78579/1‑140 (MQ=255)                                                                  ttattGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATaaa                                                                   >  2:348242/1‑140 (MQ=255)                                                                    attGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGt                                                                 <  1:628214/140‑1 (MQ=255)                                                                       gCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGaa                                                              <  1:558598/140‑1 (MQ=255)                                                                       gCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGaa                                                              <  2:717342/140‑1 (MQ=255)                                                                           gtgtCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGAt                                                          <  1:130966/140‑1 (MQ=255)                                                                           gtgtCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGAt                                                          >  1:247641/1‑140 (MQ=255)                                                                           gtgtCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGAt                                                          >  2:623761/1‑140 (MQ=255)                                                                             gtCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATcc                                                        >  2:750972/1‑140 (MQ=255)                                                                                 ggTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAg                                                    <  2:380075/140‑1 (MQ=255)                                                                                  gTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAgg                                                   <  1:826028/140‑1 (MQ=255)                                                                                  gTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAgg                                                   <  1:510157/140‑1 (MQ=255)                                                                                      cTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCAt                                               <  1:348242/140‑1 (MQ=255)                                                                                               gcgcATATTTCCGTCTCTGCGCCCCACAGCACCGGTGCGCTCGTCTCCGGTATTGCGATAACTGTCCTGGCCTATGCGGACTTTGTCACGATGGTGAACGCGGCGGATAACGTGAAAGACCCGGAGGTCATTATGCTACg                                      <  2:445589/140‑1 (MQ=255)                                                                                                          cGTCTCTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCtgg                           >  2:860224/1‑140 (MQ=255)                                                                                                            tctcTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCtggtg                         <  2:863387/140‑1 (MQ=255)                                                                                                            tctcTGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTCTCCTTGCCTCTGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCtggtg                         <  2:881925/140‑1 (MQ=255)                                                                                                               ctGCGCCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCg                      >  2:753561/1‑140 (MQ=255)                                                                                                                 gcgcCCCCCAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGAt                    <  2:876140/140‑1 (MQ=255)                                                                                                                    ccccccAGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTgg                 >  1:633884/1‑140 (MQ=255)                                                                                                                          aGCTCCGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATTGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTAc           >  2:866542/1‑140 (MQ=255)                                                                                                                              ccGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTACCACg       <  2:622087/140‑1 (MQ=255)                                                                                                                              ccGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTACCACg       <  2:633884/140‑1 (MQ=255)                                                                                                                               cGGTGCGCTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTACCACGt      <  1:866542/140‑1 (MQ=255)                                                                                                                                   gcgcTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTACCACGTTGCt  <  2:544966/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CCATGCGGTAGGGCGGCTGGAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCGGATAAAGTGAAAGATCCGCAGGTCATTATGCCACGGGCGTTTCTGGTGGCGATTGGCGTTACCACGTTGCT  >  NC_000913/513025‑513294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑