Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,586	T→C	M66T (ATG→ACG)	yhdV →	lipoprotein YhdV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,586	0	T	C	100.0%	161.2 / NA	49	M66T (ATG→ACG)	yhdV	lipoprotein YhdV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/36);  total (13/36)

TCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTAC  >  NC_000913/3418450‑3418720                                                                                                                                         |                                                                                                                                       tCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACggg                                                                                                                                     <  1:74113/140‑1 (MQ=255)   gCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGa                                                                                                                                   >  2:958129/1‑140 (MQ=255)     ccTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGatc                                                                                                                                 <  1:131062/140‑1 (MQ=255)           ttCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGct                                                                                                                           <  1:668279/140‑1 (MQ=255)                    aTGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGaaa                                                                                                                  <  1:59356/140‑1 (MQ=255)                           tCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGa                                                                                                           >  2:21887/1‑140 (MQ=255)                            cAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGaa                                                                                                          >  1:835450/1‑140 (MQ=255)                             aGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACAGAAc                                                                                                         <  2:406718/140‑1 (MQ=255)                                   gtcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGATGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGgcg                                                                                                   <  1:944319/140‑1 (MQ=255)                                   gtcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCACCGCTCTATCGAAATAACTGAACAGGgcg                                                                                                   <  1:958129/140‑1 (MQ=255)                                    tcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGGTGCTTTGGCTGGCCTGACGACCGGGATCATCGGTCTATCGAAATAACTGAACAgggggg                                                                                                  >  2:650272/1‑137 (MQ=255)                                    tcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTTCTGGCCCGACGACGGGGGTCATCGCTCTATCGAAATAACTGCACAAGgcgc                                                                                                  >  2:822890/1‑140 (MQ=255)                                    tcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGgcgc                                                                                                  >  2:66467/1‑140 (MQ=255)                                    tcTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGgcgc                                                                                                  >  1:14925/1‑140 (MQ=255)                                     cTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGcgcg                                                                                                 <  1:458789/140‑1 (MQ=255)                                         ttCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGt                                                                                             >  2:110900/1‑140 (MQ=255)                                               ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       <  2:5114/140‑1 (MQ=255)                                               ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       <  1:970866/140‑1 (MQ=255)                                               ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       <  1:628406/140‑1 (MQ=255)                                               ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       <  1:650272/140‑1 (MQ=255)                                               ccAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       <  1:66467/140‑1 (MQ=255)                                                  aTACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGctc                                                                                    >  2:775069/1‑140 (MQ=255)                                                      cTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCtttt                                                                                <  1:110900/140‑1 (MQ=255)                                                       tGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       <  1:157188/132‑1 (MQ=255)                                                       tGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCcgcg                                                                                       >  2:157188/1‑132 (MQ=255)                                                         tATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                             <  1:775069/140‑1 (MQ=255)                                                         tATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                             <  2:625680/140‑1 (MQ=255)                                                         tATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTaa                                                                             <  1:21887/140‑1 (MQ=255)                                                          aTGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAAc                                                                            >  1:894827/1‑140 (MQ=255)                                                              aaGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATa                                                                        <  2:819309/140‑1 (MQ=255)                                                               aGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATAc                                                                       <  2:14925/140‑1 (MQ=255)                                                                ggCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACa                                                                      >  2:615479/1‑140 (MQ=255)                                                                ggCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACa                                                                      >  2:829131/1‑140 (MQ=255)                                                                   aCGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTa                                                                   >  1:600460/1‑140 (MQ=255)                                                                    cGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAt                                                                  <  1:314424/140‑1 (MQ=255)                                                                      aCTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTAtct                                                                <  2:333643/140‑1 (MQ=255)                                                                          aGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAg                                                                     >  1:409693/1‑131 (MQ=255)                                                                          aGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAg                                                                     <  2:409693/131‑1 (MQ=255)                                                                           ggATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCa                                                           <  2:894827/140‑1 (MQ=255)                                                                                 cTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGAt                                                     >  1:876134/1‑140 (MQ=255)                                                                                     gAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaaaa                                                 <  1:829131/140‑1 (MQ=255)                                                                                     gAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATaaaa                                                 >  1:778459/1‑140 (MQ=255)                                                                                      aaaCGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTTCTGGCCTGACGACGGGGGTCATCGCTCTATCGAAATAACTGAACAAGGCGCGGAGGTCCGCGCTCTTTTTTACGATACAGTATCTCTCCATAAGATaaaaa                                                >  2:477812/1‑139 (MQ=255)                                                                                       aaCGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAata                                               <  2:245330/140‑1 (MQ=255)                                                                                           gCTGGGGCTATTGCCGGCGGGGGAGCTGCTGTTGCTGGCCTGACGACGGGGGTCATCGCTCTATCGAAATAACTGAACAGGGCGCGGGGGTCCGCGCTCTTTTTTACGATACAGTATCTTTCCATAAGATAAAATAtttt                                           >  2:232008/1‑138 (MQ=255)                                                                                              ggggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCtat                                        >  2:648911/1‑140 (MQ=255)                                                                                              ggggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCtat                                        <  2:778459/140‑1 (MQ=255)                                                                                              ggggCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGAGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCtat                                        <  1:539196/140‑1 (MQ=255)                                                                                                    aTTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTctg                                  <  1:477812/140‑1 (MQ=255)                                                                                                         cggcggGGCAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTggg                             <  1:914818/140‑1 (MQ=255)                                                                                                           gcggGGCAGCTGCTGTTGCTGGCCTGACGACGGGGGTCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTa                           >  2:467354/1‑140 (MQ=255)                                                                                                                 cAGCTGCTGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTccc                     <  2:876134/140‑1 (MQ=255)                                                                                                                   gctgctGTTGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCctg                   <  1:648911/140‑1 (MQ=255)                                                                                                                           tGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTa           <  1:467354/140‑1 (MQ=255)                                                                                                                           tGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTa           <  2:761031/140‑1 (MQ=255)                                                                                                                           tGCTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTa           <  1:615479/140‑1 (MQ=255)                                                                                                                             cTGGCCTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGc         <  2:610619/140‑1 (MQ=255)                                                                                                                                 ccTGACGACGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGt     >  1:698310/1‑140 (MQ=255)                                                                                                                                    gacgacGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGATCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTaa  >  1:33049/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       TCGCCCTGCGTTCCGGTTTATGACGATCAGGGTCGTCTGGTTCACACCAATACCTGTATGAAAGGCACGACTCAGGATAACTGGGAAACGGCTGGGGCTATTGCCGGCGGGGCAGCTGCTGTTGCTGGCCTGACGATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTAC  >  NC_000913/3418450‑3418720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑