Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,452,342	A→G	G195G (GGT→GGC)	rplC ←	50S ribosomal subunit protein L3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,452,342	0	A	G	100.0%	185.6 / NA	53	G195G (GGT→GGC)	rplC	50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/23);  total (30/23)

GCTTCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGT  >  NC_000913/3452210‑3452476                                                                                                                                     |                                                                                                                                       gcTTCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGa                                                                                                                                 <  2:962633/140‑1 (MQ=255)  cTTCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCGGGGAc                                                                                                                                <  1:892026/140‑1 (MQ=255)    tCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAg                                                                                                                              <  2:857617/140‑1 (MQ=255)              cACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAAc                                                                                                                    <  2:452021/140‑1 (MQ=255)              cACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAAc                                                                                                                    <  1:368859/140‑1 (MQ=255)                               tCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCt                                                                                                   <  1:66421/140‑1 (MQ=255)                                  gAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCt                                                                                                   <  2:975043/137‑1 (MQ=255)                                  gAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCt                                                                                                   >  1:975043/1‑137 (MQ=255)                                  gAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCt                                                                                                   >  1:96619/1‑137 (MQ=255)                                  gAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCt                                                                                                   <  2:96619/137‑1 (MQ=255)                                       aGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCa                                                                                           >  1:302187/1‑140 (MQ=255)                                               cgcTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGtac                                                                                   >  2:469449/1‑140 (MQ=255)                                               cgcTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGtac                                                                                   <  2:302187/140‑1 (MQ=255)                                                gcTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGtact                                                                                  >  1:27516/1‑140 (MQ=255)                                                      cgcgTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCa                                                                            <  1:274254/140‑1 (MQ=255)                                                       gcgTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCa                                                                            >  2:848524/1‑139 (MQ=255)                                                       gcgTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCa                                                                            <  1:848524/139‑1 (MQ=255)                                                           cTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGct                                                                       <  1:216424/140‑1 (MQ=255)                                                             ttCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGctct                                                                     <  2:874014/140‑1 (MQ=255)                                                                    cTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGt                                                              >  2:570080/1‑140 (MQ=255)                                                                     tAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGtt                                                             >  2:812252/1‑140 (MQ=255)                                                                       aTTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTac                                                           >  2:292/1‑140 (MQ=255)                                                                            aTTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTaca                                                          <  1:551798/136‑1 (MQ=255)                                                                            aTTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTaca                                                          >  2:551798/1‑136 (MQ=255)                                                                            aTTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACAcgtt                                                      <  2:20258/140‑1 (MQ=255)                                                                                 tATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTa                                                 <  2:27516/140‑1 (MQ=255)                                                                                   tctcCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTAcc                                               >  1:402329/1‑140 (MQ=255)                                                                                       cTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAGCCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATc                                           >  2:865894/1‑140 (MQ=255)                                                                                          aCGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGa                                        >  2:240027/1‑140 (MQ=255)                                                                                             ccTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCt                                     >  1:138421/1‑140 (MQ=255)                                                                                               ttCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACAcgttcgt                                                   >  1:408414/1‑124 (MQ=255)                                                                                               ttCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACAcgttcgt                                                   <  2:408414/124‑1 (MQ=255)                                                                                               ttCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGc                                   >  2:569921/1‑140 (MQ=255)                                                                                                 cacaGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCa                                 >  2:901865/1‑140 (MQ=255)                                                                                                 cacaGCTGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCa                                 >  2:847902/1‑140 (MQ=255)                                                                                                       tGGTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCt                           <  1:47954/140‑1 (MQ=255)                                                                                                        ggTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCtt                          >  1:877550/1‑140 (MQ=255)                                                                                                         gTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTg                         <  1:847902/140‑1 (MQ=255)                                                                                                         gTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTg                         >  2:157233/1‑140 (MQ=255)                                                                                                         gTTTAACGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTg                         >  1:503743/1‑140 (MQ=255)                                                                                                              aCGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCttt                    >  2:594250/1‑140 (MQ=255)                                                                                                               cGATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTg                   <  2:503743/140‑1 (MQ=255)                                                                                                                gATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGa                  >  2:339868/1‑140 (MQ=255)                                                                                                                gATCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGa                  <  1:469449/140‑1 (MQ=255)                                                                                                                 aTCAGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGaa                 >  2:920717/1‑140 (MQ=255)                                                                                                                    aGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAacac              >  1:839369/1‑140 (MQ=255)                                                                                                                    aGGTCGCTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAacac              >  2:494216/1‑140 (MQ=255)                                                                                                                          cTACCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGcc        >  1:648650/1‑140 (MQ=255)                                                                                                                            aCCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCg      >  2:822234/1‑140 (MQ=255)                                                                                                                            aCCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCg      >  1:701010/1‑140 (MQ=255)                                                                                                                            aCCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCg      <  1:812252/140‑1 (MQ=255)                                                                                                                            aCCGGTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCg      <  1:157233/140‑1 (MQ=255)                                                                                                                                gTTGCGCCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGt  >  1:299153/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GCTTCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGT  >  NC_000913/3452210‑3452476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑