Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,504,478	T→C	F143L (TTT→CTT)	frlR →	putative DNA‑binding transcriptional regulator FrlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,504,478	0	T	C	100.0%	197.3 / NA	56	F143L (TTT→CTT)	frlR	putative DNA‑binding transcriptional regulator FrlR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

GGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATA  >  NC_000913/3504343‑3504613                                                                                                                                        |                                                                                                                                         ggcaaggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTAt                                                                                                                                      >  1:950868/1‑140 (MQ=255)    aaggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGa                                                                                                                                   <  2:950868/140‑1 (MQ=255)    aaggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGa                                                                                                                                   <  1:143071/140‑1 (MQ=255)    aaggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGa                                                                                                                                   <  2:305924/140‑1 (MQ=255)     aggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGAt                                                                                                                                  >  1:779224/1‑140 (MQ=255)      ggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTg                                                                                                                                    <  2:840538/137‑1 (MQ=255)      ggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTg                                                                                                                                    >  1:840538/1‑137 (MQ=255)      ggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATa                                                                                                                                 >  2:570358/1‑140 (MQ=255)       gcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGTGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATa                                                                                                                                 >  2:366631/1‑139 (MQ=255)       gcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGTGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATa                                                                                                                                 <  1:366631/139‑1 (MQ=255)           cgaAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGa                                                                                                                                   >  2:623461/1‑133 (MQ=255)           cgaAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGa                                                                                                                                   <  1:623461/133‑1 (MQ=255)                             aaCAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTcgcg                                                                                                           >  1:160539/1‑139 (MQ=255)                             aaCAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTcgcg                                                                                                           <  2:160539/139‑1 (MQ=255)                             aaCAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGt                                                                                                          <  2:586729/140‑1 (MQ=255)                             aaCAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGt                                                                                                          <  1:570358/140‑1 (MQ=255)                              aCAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGtt                                                                                                         >  1:332971/1‑140 (MQ=255)                                 ggAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATc                                                                                                      >  2:515168/1‑140 (MQ=255)                                       ggTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACt                                                                                                >  2:103280/1‑140 (MQ=255)                                            gcgccgcgccGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTtgat                                                                                           <  1:132821/140‑1 (MQ=255)                                            gcgccgcgccGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTtgat                                                                                           <  1:59081/140‑1 (MQ=255)                                                  cgccgTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTTATGAGAtt                                                                                     >  2:265862/1‑140 (MQ=255)                                                            cGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaag                                                                           <  2:332971/140‑1 (MQ=255)                                                              aaaaGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaagga                                                                         <  1:515168/140‑1 (MQ=255)                                                                 aGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGc                                                                      >  1:286187/1‑140 (MQ=255)                                                                 aGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGCGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGc                                                                      >  1:689564/1‑140 (MQ=255)                                                                   cTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGCGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTc                                                                    <  2:689564/140‑1 (MQ=255)                                                                     gAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCa                                                                  <  1:690506/140‑1 (MQ=255)                                                                        cATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCt                                                              <  1:255072/140‑1 (MQ=255)                                                                         aTCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTa                                                             <  1:759346/140‑1 (MQ=255)                                                                            cccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCa                                                          <  1:422061/140‑1 (MQ=255)                                                                             ccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaag                                                                           >  1:974955/1‑123 (MQ=255)                                                                             ccGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaag                                                                           <  2:974955/123‑1 (MQ=255)                                                                              cGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGt                                                        <  2:344029/140‑1 (MQ=255)                                                                                gAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTa                                                      >  1:292597/1‑140 (MQ=255)                                                                                       cGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAgg                                               <  2:779224/140‑1 (MQ=255)                                                                                               tCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGa                                              <  1:970514/133‑1 (MQ=255)                                                                                               tCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGa                                              >  2:970514/1‑133 (MQ=255)                                                                                               tCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGtttt                                       >  1:84751/1‑140 (MQ=255)                                                                                                   tctcTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGaca                                   <  1:65477/140‑1 (MQ=255)                                                                                                     tctGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGCAGCGTTTTGACAcg                                 <  2:466298/140‑1 (MQ=255)                                                                                                      ctGCCGGGTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACAcgc                                >  2:162630/1‑140 (MQ=255)                                                                                                             gTGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGt                         <  1:162630/140‑1 (MQ=255)                                                                                                              tGATGTATCTCGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTc                        >  2:772172/1‑140 (MQ=255)                                                                                                                        cGATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaaaa              >  2:615715/1‑140 (MQ=255)                                                                                                                         gATAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaaaaa             <  1:615715/140‑1 (MQ=255)                                                                                                                          aTAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaaaaa             >  1:777933/1‑140 (MQ=255)                                                                                                                          aTAAAGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAg            >  1:148089/1‑140 (MQ=255)                                                                                                                            aaaGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGt                         >  1:979076/1‑125 (MQ=255)                                                                                                                            aaaGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGt                         <  2:979076/125‑1 (MQ=255)                                                                                                                            aaaGAGCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGAc          <  1:109984/140‑1 (MQ=255)                                                                                                                                agCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGc                     <  2:388627/125‑1 (MQ=255)                                                                                                                                agCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGc                     >  1:388627/1‑125 (MQ=255)                                                                                                                                agCCGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATc      >  2:122133/1‑140 (MQ=255)                                                                                                                                   cGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGat   >  1:906572/1‑140 (MQ=255)                                                                                                                                   cGCTGCTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGat   >  2:838837/1‑140 (MQ=255)                                                                                                                                    gctgctTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTTATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGata  <  1:265862/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCA‑CCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATA  >  NC_000913/3504343‑3504613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑