Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,600,218 T→C D189G (GAT→GGT)  rpoH ← RNA polymerase, sigma 32 (sigma H) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,600,2180TC100.0% 141.6 / NA 42D189G (GAT→GGT) rpoHRNA polymerase, sigma 32 (sigma H) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

GACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAATCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGTTAAACCAGCCCAGACG  >  NC_000913/3600090‑3600351
                                                                                                                                |                                                                                                                                     
gACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGaa                                                                                                                            >  1:106613/1‑140 (MQ=255)
gACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGaa                                                                                                                            >  1:243533/1‑140 (MQ=255)
 aCCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAAccgt                                                                                                                                    >  1:13585/1‑131 (MQ=255)
 aCCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAAccgt                                                                                                                                    <  2:13585/131‑1 (MQ=255)
  cccTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGa                                                                                                                          <  1:217353/140‑1 (MQ=255)
  cccTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGa                                                                                                                          <  2:482471/140‑1 (MQ=255)
            cgTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAg                                                                                                                <  2:828896/140‑1 (MQ=255)
                                    gCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCtgtg                                                                                                   >  1:240668/1‑129 (MQ=255)
                                    gCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCtgtg                                                                                                   <  2:240668/129‑1 (MQ=255)
                                      tctTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGa                                                                                      <  1:548434/140‑1 (MQ=255)
                                       ctTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGAt                                                                                     <  2:106613/140‑1 (MQ=255)
                                             ggTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCAtc                                                                               <  2:967382/139‑1 (MQ=255)
                                                    atcTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTGGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCacgta                                                                        >  2:162899/1‑140 (MQ=255)
                                                       ttCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCacgtacgt                                                                     <  1:286877/140‑1 (MQ=255)
                                                       ttCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCacgtacgt                                                                     >  1:884862/1‑140 (MQ=255)
                                                            tGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTg                                                                <  2:48478/140‑1 (MQ=255)
                                                                 tCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGt                                                           <  2:536805/140‑1 (MQ=255)
                                                                   ggCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTa                                                         >  2:614288/1‑140 (MQ=255)
                                                                       aaGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTGGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGcc                                                     <  1:162899/140‑1 (MQ=255)
                                                                       aaGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGcc                                                     <  2:243533/140‑1 (MQ=255)
                                                                        aGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGccc                                                    >  1:44525/1‑140 (MQ=255)
                                                                           tAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGt                                                 >  2:131657/1‑140 (MQ=255)
                                                                             gatgatTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTc                                               <  2:44525/140‑1 (MQ=255)
                                                                              atgatTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCa                                              <  2:521575/140‑1 (MQ=255)
                                                                                  tttATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACggg                                          >  1:406683/1‑140 (MQ=255)
                                                                                   ttATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGc                                         >  2:346672/1‑140 (MQ=255)
                                                                                     aTCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGcca                                       <  2:406683/140‑1 (MQ=255)
                                                                                           cAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCa                                              <  1:456265/127‑1 (MQ=255)
                                                                                           cAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCa                                              >  2:456265/1‑127 (MQ=255)
                                                                                           cAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCAttt                                 >  1:709774/1‑140 (MQ=255)
                                                                                           cAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCAttt                                 >  1:603720/1‑140 (MQ=255)
                                                                                                     aCCGGAGCCATCGGCTGGCTGTCGGATCCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATc                       <  2:238963/140‑1 (MQ=255)
                                                                                                     aCCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATc                       <  2:603720/140‑1 (MQ=255)
                                                                                                     aCCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATc                       >  1:288748/1‑140 (MQ=255)
                                                                                                     aCCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTAAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCAt                        <  1:13919/139‑1 (MQ=255)
                                                                                                     aCCGGAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTAAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCAt                        >  2:13919/1‑139 (MQ=255)
                                                                                                        ggAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTg                    >  2:928790/1‑140 (MQ=255)
                                                                                                        ggAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTg                    >  2:624294/1‑140 (MQ=255)
                                                                                                         gAGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTgg                   >  1:497236/1‑140 (MQ=255)
                                                                                                          aGCCATCGGCTGGCTGTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGt                  <  1:624294/140‑1 (MQ=255)
                                                                                                                    tggctgTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGTTAAAcc            <  2:590500/136‑1 (MQ=255)
                                                                                                                    tggctgTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGTTAAAcc            >  1:590500/1‑136 (MQ=255)
                                                                                                                         gTCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGTTAAACCAGCCCAGAc   >  1:42932/1‑140 (MQ=255)
                                                                                                                          tCGGAACCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGTTAAACCAGCCCAGACg  <  1:131657/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
GACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGCTGGCTGTCGGAATCGTCGTCGGAAGACAGGTCAAAGGTCATGTCCTGTGCCGCCATACGTGATTCCATCTCACGTACGTCTTTGCTGGTTACGCCCAGTTCACGGGCCACCATTTCGACTTCATCCTGGTTAAACCAGCCCAGACG  >  NC_000913/3600090‑3600351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑