Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,608,461	A→G	M671V (ATG→GTG)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,608,461	0	A	G	100.0%	132.9 / NA	39	M671V (ATG→GTG)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/22);  total (17/22)

GGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGAT  >  NC_000913/3608325‑3608592                                                                                                                                         |                                                                                                                                    ggTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGa                                                                                                                                  <  2:852261/140‑1 (MQ=255)   tCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGAtt                                                                                                                                >  2:721511/1‑140 (MQ=255)      gTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGaa                                                                                                                             <  1:950982/140‑1 (MQ=255)           ggTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGc                                                                                                                        >  1:959302/1‑140 (MQ=255)            gTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCa                                                                                                                       >  2:817050/1‑140 (MQ=255)             tATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCAc                                                                                                                      >  2:443324/1‑140 (MQ=255)                tAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCAcgcg                                                                                                                   <  1:450413/140‑1 (MQ=255)                     acgCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACt                                                                                                              <  1:54175/140‑1 (MQ=255)                       gcgcCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCa                                                                                                            >  1:229287/1‑140 (MQ=255)                       gcgcCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCa                                                                                                            <  1:289361/140‑1 (MQ=255)                       gagcCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTgg                                                                                                                         >  2:117552/3‑127 (MQ=255)                       gagcCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTgg                                                                                                                         <  1:117552/125‑1 (MQ=255)                               gATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAata                                                                                                    <  1:418552/140‑1 (MQ=255)                                    aaGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGc                                                                                               <  1:910393/140‑1 (MQ=255)                                       cTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAg                                                                                            <  2:667775/140‑1 (MQ=255)                                         gccgccATCGGGATTGAAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTACCCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGaa                                                                                          <  2:816152/140‑1 (MQ=255)                                           cgccATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACa                                                                                        >  2:464171/1‑140 (MQ=255)                                                      ttGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTgcg                                                                             <  1:303757/140‑1 (MQ=255)                                                         cAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTg                                                                          >  1:435004/1‑140 (MQ=255)                                                                tAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa                                                                   <  1:436709/140‑1 (MQ=255)                                                                tAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa                                                                   >  2:436709/1‑140 (MQ=255)                                                                        caGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATc                                                           >  1:768461/1‑140 (MQ=255)                                                                                gcgcTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGt                                                   <  1:975865/140‑1 (MQ=255)                                                                                  gcTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTca                                                 <  2:959302/140‑1 (MQ=255)                                                                                     ggAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcacca                                              <  2:229287/140‑1 (MQ=255)                                                                                        aaCCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGc                                           <  2:392507/140‑1 (MQ=255)                                                                                             ccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTAACCACGCTGTTa                                      >  2:612111/1‑140 (MQ=255)                                                                                              cgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAg                                     <  1:721511/140‑1 (MQ=255)                                                                                              cgACGCACCATTAACCCATACCCCGCTTCGCGGCATGGTCGAAGGGATTGAACTGGCACGCGCCCCTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAg                                     <  2:164198/140‑1 (MQ=255)                                                                                                     gcaTTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAc                              <  1:817050/140‑1 (MQ=255)                                                                                                       aTTAACCCATAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCg                            >  1:574220/1‑140 (MQ=255)                                                                                                                tAACCACCTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGtggc                   >  2:773159/1‑140 (MQ=255)                                                                                                                    ccccTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGtggctggc               <  2:435004/138‑1 (MQ=255)                                                                                                                     accTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCa              <  1:443324/140‑1 (MQ=255)                                                                                                                      ccTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAg             >  1:431544/1‑140 (MQ=255)                                                                                                                       cTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCCATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAc                              <  2:607589/122‑1 (MQ=255)                                                                                                                       cTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAc                              >  1:607589/1‑122 (MQ=255)                                                                                                                       cTGCGCGGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGt            >  2:163218/1‑140 (MQ=255)                                                                                                                            cgGCCTGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTgg       >  2:29186/1‑140 (MQ=255)                                                                                                                                 tGGTGCAAGTGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGAt  <  1:773159/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GGTCGGTGACGGTATTAACGACGCGCCAGCGATGAAAGCTGCCGCCATCGGGATTGCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGAT  >  NC_000913/3608325‑3608592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑