Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 543,854 T→C T65A (ACA→GCA)  allE ← (S)‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,8540TC100.0% 145.3 / NA 42T65A (ACA→GCA) allE(S)‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

TAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTACCGTGTTTAACAA  >  NC_000913/543724‑543988
                                                                                                                                  |                                                                                                                                      
tAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATa                                                                                                                               <  2:890541/140‑1 (MQ=255)
 aGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATaa                                                                                                                              >  1:612247/1‑140 (MQ=255)
     aCCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGCCCAGATAATcaa                                                                                                                          >  2:143237/1‑140 (MQ=255)
      ccTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATcaac                                                                                                                         <  1:506335/140‑1 (MQ=255)
            cTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAaga                                                                                                                   >  2:481347/1‑140 (MQ=255)
                 ggCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATa                                                                                                                               <  2:774884/123‑1 (MQ=255)
                 ggCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATa                                                                                                                               >  1:774884/1‑123 (MQ=255)
                     aaTGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAg                                                                                                          <  2:189411/140‑1 (MQ=255)
                          tttGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTg                                                                                                     <  2:48402/140‑1 (MQ=255)
                            tGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGc                                                                                                   <  2:85372/140‑1 (MQ=255)
                            tGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGc                                                                                                   <  2:612247/140‑1 (MQ=255)
                            tGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGc                                                                                                   >  1:518430/1‑140 (MQ=255)
                               cTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTg                                                                                                <  1:705789/140‑1 (MQ=255)
                                   ggCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGgaga                                                                                            <  1:152464/140‑1 (MQ=255)
                                        tGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCACCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGAtt                                                                                       >  1:19684/1‑140 (MQ=255)
                                               gATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGt                                                                                >  2:375121/1‑140 (MQ=255)
                                                          agaTCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCa                                                                     >  1:464627/1‑140 (MQ=255)
                                                            aTCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCaaa                                                                   >  2:199649/1‑140 (MQ=255)
                                                               acaTACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGcc                                                                >  1:890325/1‑140 (MQ=255)
                                                               acaTACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGcc                                                                <  1:199649/140‑1 (MQ=255)
                                                                 aTACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCg                                                              >  2:784089/1‑140 (MQ=255)
                                                                      ggAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGaata                                                         >  2:63475/1‑140 (MQ=255)
                                                                            tttCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATAttt                                                   <  1:481347/140‑1 (MQ=255)
                                                                                     cTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGa                                          >  2:561795/1‑140 (MQ=255)
                                                                                       tCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGAcc                                        >  2:447868/1‑140 (MQ=255)
                                                                                        cGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCa                                       >  2:791319/1‑140 (MQ=255)
                                                                                         gCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCAt                                      <  1:63475/140‑1 (MQ=255)
                                                                                         gCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCAt                                      <  2:464627/140‑1 (MQ=255)
                                                                                          ccACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATc                                     <  1:791319/140‑1 (MQ=255)
                                                                                           cACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCCTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATcc                                    <  2:823081/140‑1 (MQ=255)
                                                                                            aCCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCg                                   <  2:484444/140‑1 (MQ=255)
                                                                                              cGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCggg                                 >  2:451507/1‑140 (MQ=255)
                                                                                                  gCCCTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTa                             <  1:447868/140‑1 (MQ=255)
                                                                                                   cccTGTTGGTTGCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTaa                            >  1:938525/1‑140 (MQ=255)
                                                                                                          ggttgCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCg                     >  1:789643/1‑140 (MQ=255)
                                                                                                             tgCCACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGaaa                  >  2:487104/1‑140 (MQ=255)
                                                                                                                cACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTa               <  2:19684/140‑1 (MQ=255)
                                                                                                                cACCGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTa               <  1:784089/140‑1 (MQ=255)
                                                                                                                  ccGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTAcc             >  1:795647/1‑140 (MQ=255)
                                                                                                                  ccGTTTTGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTAcc             >  1:549058/1‑140 (MQ=255)
                                                                                                                     ttttGATGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTACCgtg          >  2:140126/1‑140 (MQ=255)
                                                                                                                           tGCAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTACCGTGTTTAAc    <  2:938525/140‑1 (MQ=255)
                                                                                                                             cAGTGCGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTACCGTGTTTAACaa  >  2:275367/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                      
TAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAGAGGATTGTCGCGTCACAATTTTCAAAGCCCGGAATAATATTTTTTACCAGACCATCCGGGGTTAACAGTGCGAAATTACCGTGTTTAACAA  >  NC_000913/543724‑543988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑