Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,672	T→C	Y483C (TAC→TGC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,672	0	T	C	100.0%	188.0 / NA	54	Y483C (TAC→TGC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/28);  total (26/28)

GTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCC  >  NC_000913/3878536‑3878797                                                                                                                                         |                                                                                                                              gTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACt                                                                                                                            >  1:620505/1‑140 (MQ=255)      cGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCa                                                                                                                       >  2:224499/1‑140 (MQ=255)      cGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCa                                                                                                                       <  1:946144/140‑1 (MQ=255)       gTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCAc                                                                                                                      <  1:413667/140‑1 (MQ=255)        tGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACg                                                                                                                     <  2:572914/140‑1 (MQ=255)          aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGAc                                                                                                                   >  1:601853/1‑140 (MQ=255)          aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGAc                                                                                                                   >  1:226886/1‑140 (MQ=255)          aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGAc                                                                                                                   >  2:533348/1‑140 (MQ=255)          aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGAc                                                                                                                   >  2:15743/1‑140 (MQ=255)          aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGAc                                                                                                                   >  2:627432/1‑140 (MQ=255)           ccGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGAcc                                                                                                                  <  2:245147/140‑1 (MQ=255)                   aaCGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACcac                                                                                                          <  2:601853/140‑1 (MQ=255)                          tCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAg                                                                                                   >  1:739459/1‑140 (MQ=255)                          tCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAg                                                                                                   <  1:224499/140‑1 (MQ=255)                            cGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAgcg                                                                                                 <  1:353449/140‑1 (MQ=255)                            cGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAgcg                                                                                                 <  2:739459/140‑1 (MQ=255)                            cGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAgcg                                                                                                 <  2:456695/140‑1 (MQ=255)                                           gaagaaGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCg                                                                                  >  1:224053/1‑140 (MQ=255)                                             agaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGcc                                                                                <  2:465263/140‑1 (MQ=255)                                               aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                              <  1:900708/140‑1 (MQ=255)                                               aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                              <  2:226886/140‑1 (MQ=255)                                                agGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                             <  2:224053/140‑1 (MQ=255)                                                 ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                            >  1:367124/1‑140 (MQ=255)                                                      aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTga                                                                       >  1:515328/1‑140 (MQ=255)                                                      aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTga                                                                       >  2:759146/1‑140 (MQ=255)                                                       cagcagCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgag                                                                      <  2:515328/140‑1 (MQ=255)                                                         gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaa                                                                    <  2:838223/140‑1 (MQ=255)                                                           agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                                  <  2:56745/140‑1 (MQ=255)                                                           agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                                  <  2:422884/140‑1 (MQ=255)                                                            gcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagag                                                                 >  2:636749/1‑140 (MQ=255)                                                                aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCa                                                             <  1:572727/140‑1 (MQ=255)                                                                aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCa                                                             <  1:476759/140‑1 (MQ=255)                                                                aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCa                                                             <  2:801327/140‑1 (MQ=255)                                                                 cGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAt                                                            >  1:463451/1‑140 (MQ=255)                                                                     tgtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                        <  2:521732/140‑1 (MQ=255)                                                                       tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                                      >  2:628547/1‑140 (MQ=255)                                                                                tCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                             >  1:156523/1‑140 (MQ=255)                                                                                          cATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACg                                   >  2:867268/1‑140 (MQ=255)                                                                                          cATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACg                                   >  1:292180/1‑140 (MQ=255)                                                                                              ggTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                               <  1:15743/140‑1 (MQ=255)                                                                                                            tgCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc                 <  2:463451/140‑1 (MQ=255)                                                                                                            tgCTGTGATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc                 >  2:547090/1‑140 (MQ=255)                                                                                                               tgtgATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc              >  1:448024/1‑140 (MQ=255)                                                                                                               tgtgATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc              >  1:287252/1‑140 (MQ=255)                                                                                                                 tgATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg            <  1:951150/140‑1 (MQ=255)                                                                                                                 tgATAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTACAGCgg            >  1:425613/1‑140 (MQ=255)                                                                                                                   aTAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa          <  2:614616/140‑1 (MQ=255)                                                                                                                   aTAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa          >  1:31196/1‑140 (MQ=255)                                                                                                                   aTAACGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa          >  2:856873/1‑140 (MQ=255)                                                                                                                     aaCGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGa        <  2:287252/140‑1 (MQ=255)                                                                                                                      aCGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGaa       <  2:31196/140‑1 (MQ=255)                                                                                                                      aCGCAGTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTAGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGaa       <  2:166936/140‑1 (MQ=255)                                                                                                                           gTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGcc  >  2:188773/1‑140 (MQ=255)                                                                                                                           gTTTGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGcc  >  1:239130/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                              GTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCC  >  NC_000913/3878536‑3878797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑