Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,893,684	T→C	L307S (TTA→TCA)	yidZ →	putative LysR‑type transcriptional regulator YidZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,893,684	0	T	C	100.0%	204.0 / NA	59	L307S (TTA→TCA)	yidZ	putative LysR‑type transcriptional regulator YidZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/32);  total (27/32)

ACTACAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTC  >  NC_000913/3893549‑3893819                                                                                                                                        |                                                                                                                                        actacAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACgg                                                                                                                                     >  1:34314/1‑140 (MQ=255)   tacAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGACGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGa                                                                                                                                   <  2:864718/140‑1 (MQ=255)   tacAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGa                                                                                                                                   <  1:173457/140‑1 (MQ=255)   tacAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGa                                                                                                                                   >  1:48955/1‑140 (MQ=255)      aaTCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAAc                                                                                                                                <  2:974051/140‑1 (MQ=255)      aaTCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAAc                                                                                                                                <  1:386173/140‑1 (MQ=255)          aaCTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCAtt                                                                                                                            >  2:687954/1‑140 (MQ=255)          aaCTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCAtt                                                                                                                            >  1:494069/1‑140 (MQ=255)          aaCTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCAtt                                                                                                                            >  1:397912/1‑140 (MQ=255)          aaCGCCATCAATTGCTGTTGGTTGCTTTTCCGCCCCCGTTTGACGAAAACCAACAAAAAAAACTGTAAGTTCCTTTTACCCTGCTGTGGCATAAACGGCATAGCCATAATCCGAAGAACGTCTGGCCAAGGGTAACCAtt                                                                                                                            >  2:484299/1‑140 (MQ=255)            cTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTaa                                                                                                                          >  1:213124/1‑140 (MQ=255)                 tCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAAcc                                                                                                                     >  2:661565/1‑140 (MQ=255)                 tCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAAC‑GAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCt                                                                                                                    >  2:246379/1‑140 (MQ=255)                        ccGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTAcgc                                                                                                              >  2:153744/1‑140 (MQ=255)                           ttggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTc                                                                                                           >  1:518549/1‑140 (MQ=255)                           ttggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTc                                                                                                           <  1:661565/140‑1 (MQ=255)                           ttggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAAC‑GAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCg                                                                                                          <  1:246379/140‑1 (MQ=255)                            tggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCg                                                                                                          <  2:855806/140‑1 (MQ=255)                                tgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATgg                                                                                                      <  2:802200/140‑1 (MQ=255)                                     ttCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATaa                                                                                                 <  2:728598/140‑1 (MQ=255)                                       ccTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAAcc                                                                                               >  2:839479/1‑140 (MQ=255)                                         tctcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGa                                                                                             <  2:603321/140‑1 (MQ=255)                                          ctcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGa                                                                                                         >  1:968414/1‑127 (MQ=255)                                          ctcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGa                                                                                                         <  2:968414/127‑1 (MQ=255)                                          ctcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGaa                                                                                            <  1:112342/140‑1 (MQ=255)                                             ccGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAATAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCg                                                                                         >  1:859568/1‑140 (MQ=255)                                                       aaaGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAAcgc                                                                               <  2:431840/140‑1 (MQ=255)                                                                aaaaaaaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAAtt                                                                      <  1:476313/140‑1 (MQ=255)                                                                 aaaaatGCTGGAAGTTCTTTTTACCAGGCTGTGCCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCCCGCGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGCACCGCGACCCAAttt                                                                     <  2:574476/140‑1 (MQ=255)                                                                        cTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATa                                                              <  2:34314/140‑1 (MQ=255)                                                                                 ccTTTTACCCTGCTGGGGCATAAACGGAACAGCCATAATCCGAAAATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTaaa                                                     <  2:283915/140‑1 (MQ=255)                                                                                     ttACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAg                                                 <  1:781157/140‑1 (MQ=255)                                                                                       aCCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGtt                                               >  2:116147/1‑140 (MQ=255)                                                                                          ctgctgTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGta                                            <  1:786741/140‑1 (MQ=255)                                                                                           tgctgTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaa                                           <  2:275244/140‑1 (MQ=255)                                                                                             ctgTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaata                                         >  2:143501/1‑140 (MQ=255)                                                                                             ctgTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaata                                         >  2:615860/1‑140 (MQ=255)                                                                                               gtgGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGGCCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAg                                       <  2:518549/140‑1 (MQ=255)                                                                                               gtgGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAg                                       >  1:472642/1‑140 (MQ=255)                                                                                               gtgGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAg                                       <  1:687954/140‑1 (MQ=255)                                                                                                tgGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGc                                      >  2:216345/1‑140 (MQ=255)                                                                                                     tAAACGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTc                                 >  2:428775/1‑140 (MQ=255)                                                                                                       aaCGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTg                               >  2:205712/1‑140 (MQ=255)                                                                                                       aaCGGAACAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTg                               >  2:29781/1‑140 (MQ=255)                                                                                                              cAGCCATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGaa                        >  2:519959/1‑140 (MQ=255)                                                                                                                 ccATAATCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAAACGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACtt                     >  1:579520/1‑140 (MQ=255)                                                                                                                     aaTCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAg                 <  2:479191/140‑1 (MQ=255)                                                                                                                     aaTCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAg                 <  1:153744/140‑1 (MQ=255)                                                                                                                     aaTCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAg                 <  2:472642/140‑1 (MQ=255)                                                                                                                     aaTCCGAAGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAg                 <  1:615860/140‑1 (MQ=255)                                                                                                                           aaGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCtt           >  2:239610/1‑140 (MQ=255)                                                                                                                           aaGATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCtt           <  1:216345/140‑1 (MQ=255)                                                                                                                             gATCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTa         <  1:239610/140‑1 (MQ=255)                                                                                                                               tCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGt       <  2:48955/140‑1 (MQ=255)                                                                                                                               tCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGt       <  1:649971/140‑1 (MQ=255)                                                                                                                               tCGTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAAACGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGt       <  2:579520/140‑1 (MQ=255)                                                                                                                                 gTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCt     >  2:42397/1‑140 (MQ=255)                                                                                                                                 gTCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCt     >  1:283514/1‑140 (MQ=255)                                                                                                                                  tCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTg    >  2:132092/1‑140 (MQ=255)                                                                                                                                  tCTGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTg    <  2:448011/140‑1 (MQ=255)                                                                                                                                    tGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTc  <  1:205712/140‑1 (MQ=255)                                                                                                                                    tGGTCACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTc  <  1:116147/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ACTACAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTC  >  NC_000913/3893549‑3893819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑