Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,960,231	C→T	pseudogene (230/449 nt)	yifN ←	putative toxin YifN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,960,231	0	C	T	100.0%	315.2 / NA	89	pseudogene (230/449 nt)	yifN	putative toxin YifN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/47);  total (42/47)

AGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGCATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCATCGTAAT  >  NC_000913/3960097‑3960366                                                                                                                                       |                                                                                                                                        aGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTcc                                                                                                                                    <  1:423064/140‑1 (MQ=255) aGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTcc                                                                                                                                    <  1:379561/140‑1 (MQ=255)  gATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCa                                                                                                                                   >  1:927553/1‑140 (MQ=255)   aTACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCAc                                                                                                                                  >  1:655277/1‑140 (MQ=255)     aCCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGt                                                                                                                                <  2:927553/140‑1 (MQ=255)        ctctGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGtttt                                                                                                                             >  2:827785/1‑140 (MQ=255)           tGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTc                                                                                                                                     >  2:150123/1‑129 (MQ=255)           tGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTc                                                                                                                                     <  1:150123/129‑1 (MQ=255)           tGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAAc                                                                                                                          >  2:253948/1‑140 (MQ=255)           tGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAAc                                                                                                                          <  1:33125/140‑1 (MQ=255)            gTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACt                                                                                                                         >  1:226741/1‑140 (MQ=255)                   cTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTgg                                                                                                                  >  1:977349/1‑140 (MQ=255)                    ttGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGt                                                                                                                 <  1:138895/140‑1 (MQ=255)                     tGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTg                                                                                                                   >  1:736013/1‑137 (MQ=255)                     tGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTg                                                                                                                   <  2:736013/137‑1 (MQ=255)                     tGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTc                                                                                                                >  2:731959/1‑140 (MQ=255)                     tGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTc                                                                                                                >  1:248855/1‑140 (MQ=255)                     tGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTc                                                                                                                >  2:366666/1‑140 (MQ=255)                       ctctGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTcgc                                                                                                              <  1:827785/140‑1 (MQ=255)                         ctGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGa                                                                                                            <  1:731959/140‑1 (MQ=255)                          tGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTTTCTGGTCGCGAg                                                                                                           <  1:721615/140‑1 (MQ=255)                            tttAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGGATCTGCTGATCAAAGAATTGTAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTg                                                                                                         >  1:833303/1‑140 (MQ=255)                            tttAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTg                                                                                                         <  2:977349/140‑1 (MQ=255)                            tttAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTg                                                                                                         <  2:276370/140‑1 (MQ=255)                                    gTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATaa                                                                                                 >  1:820920/1‑140 (MQ=255)                                      ttCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAg                                                                                                           <  1:677374/128‑1 (MQ=255)                                      ttCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAg                                                                                                           >  2:677374/1‑128 (MQ=255)                                      ttCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATg                                                                                               >  1:300209/1‑140 (MQ=255)                                      ttCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATg                                                                                               >  2:466606/1‑140 (MQ=255)                                      ttCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATg                                                                                               >  1:762240/1‑140 (MQ=255)                                        cGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGa                                                                                             <  1:466606/140‑1 (MQ=255)                                          actgactTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAac                                                                                           >  2:473702/1‑140 (MQ=255)                                            tgactTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAacga                                                                                         <  2:226741/140‑1 (MQ=255)                                            tgactTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAacga                                                                                         <  2:20813/140‑1 (MQ=255)                                            tgactTGTTGTACCAAGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAacga                                                                                         <  1:437618/140‑1 (MQ=255)                                             gactTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAacgac                                                                                        <  2:300209/140‑1 (MQ=255)                                                        ccAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGt                                                                             >  2:228191/1‑140 (MQ=255)                                                        ccAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGt                                                                             >  2:292243/1‑140 (MQ=255)                                                        ccAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGt                                                                             <  2:344467/140‑1 (MQ=255)                                                        ccAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGt                                                                             >  1:344467/1‑140 (MQ=255)                                                        ccAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGt                                                                             <  2:820920/140‑1 (MQ=255)                                                           ggTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGc                                                                          >  2:127741/1‑140 (MQ=255)                                                            gTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGcc                                                                         <  2:248855/140‑1 (MQ=255)                                                             tCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCg                                                                              <  2:525339/134‑1 (MQ=255)                                                             tCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCg                                                                              >  1:525339/1‑134 (MQ=255)                                                             tCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCa                                                                        >  1:871899/1‑140 (MQ=255)                                                                 cTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTaa                                                                    >  2:371107/1‑140 (MQ=255)                                                                    tGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAAttt                                                                 <  1:202277/140‑1 (MQ=255)                                                                      gCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAAttttt                                                               >  1:812/1‑140 (MQ=255)                                                                           aCGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGtt                                                          >  1:928007/1‑140 (MQ=255)                                                                           aCGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGtt                                                          >  1:588387/1‑140 (MQ=255)                                                                            cGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTg                                                         <  1:702754/140‑1 (MQ=255)                                                                            cGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTg                                                         <  1:292243/140‑1 (MQ=255)                                                                                tCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCa                                                     >  1:85053/1‑140 (MQ=255)                                                                                tCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCa                                                     >  1:161808/1‑140 (MQ=255)                                                                                 ctgctgATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCAc                                                    <  1:553692/140‑1 (MQ=255)                                                                                 ctgctcATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCAc                                                    <  2:871899/140‑1 (MQ=255)                                                                                   gctgATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGa                                                  <  1:577916/140‑1 (MQ=255)                                                                                   gctgATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGa                                                  <  1:468409/140‑1 (MQ=255)                                                                                         cAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTa                                            >  2:243027/1‑140 (MQ=255)                                                                                           aaGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAAc                                          >  1:99021/1‑140 (MQ=255)                                                                                            aGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAAcc                                         <  2:588387/140‑1 (MQ=255)                                                                                                     ggTCATTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGCCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTa                                <  2:857409/140‑1 (MQ=255)                                                                                                         aTTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCAt                            <  2:781149/140‑1 (MQ=255)                                                                                                         aTTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCAt                            <  2:161808/140‑1 (MQ=255)                                                                                                         aTTGATAACATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCAt                            <  2:346589/140‑1 (MQ=255)                                                                                                               aaCATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCagg                      <  1:835622/140‑1 (MQ=255)                                                                                                                aCATTACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCagga                     >  1:71676/1‑140 (MQ=255)                                                                                                                    tACTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGaa                 >  1:63051/1‑140 (MQ=255)                                                                                                                     aCTGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAAt                <  1:127741/140‑1 (MQ=255)                                                                                                                       tGGCTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGc              >  2:275629/1‑140 (MQ=255)                                                                                                                          cTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           <  2:655277/140‑1 (MQ=255)                                                                                                                          cTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           <  2:85053/140‑1 (MQ=255)                                                                                                                          cTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           <  1:17464/140‑1 (MQ=255)                                                                                                                          cTATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCATTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           <  2:190419/140‑1 (MQ=255)                                                                                                                           tATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAAt                >  2:663011/1‑134 (MQ=255)                                                                                                                           tATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAAt                <  1:663011/134‑1 (MQ=255)                                                                                                                           tATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGcc          >  2:333452/1‑140 (MQ=255)                                                                                                                           tATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGcc          <  1:473702/140‑1 (MQ=255)                                                                                                                           tATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           >  1:181298/1‑139 (MQ=255)                                                                                                                           tATTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           <  2:181298/139‑1 (MQ=255)                                                                                                                            aTTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           >  2:654225/1‑138 (MQ=255)                                                                                                                            aTTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGc           <  1:654225/138‑1 (MQ=255)                                                                                                                            aTTTCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCa         <  1:243027/140‑1 (MQ=255)                                                                                                                             tttCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCAt        >  1:293527/1‑140 (MQ=255)                                                                                                                              ttCAACATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCATc       >  1:347293/1‑140 (MQ=255)                                                                                                                                 aaCATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCATCGTa    <  2:812/140‑1 (MQ=255)                                                                                                                                 aaCATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCATCGTa    <  1:228191/140‑1 (MQ=255)                                                                                                                                   cATGTATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCATCGTAAt  >  1:830498/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTATTTCAACATGCATTCCACGTTTTAACTTGTCTGGTCGCGAGTGGTTGATAATGGAACGACGATGCATGCGTTGCCATTAATTTTTCCGTTGAGCACGACAACTAACCTGTTTTTTATCATTTCAGGAGGAATGCGGCCATCGTAAT  >  NC_000913/3960097‑3960366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑