Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,045,892	A→G	C237C (TGT→TGC)	yihG ←	putative acyltransferase YihG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,045,892	0	A	G	97.8%	150.1 / NA	45	C237C (TGT→TGC)	yihG	putative acyltransferase YihG
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (24/20);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCG  >  NC_000913/4045756‑4046019                                                                                                                                         |                                                                                                                                ggCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACCCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAgcac                                                                                                                              >  1:677360/1‑140 (MQ=255)   cgcTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAg                                                                                                                            >  2:394671/1‑140 (MQ=255)     cTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCg                                                                                                                          <  1:778943/140‑1 (MQ=255)       tAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTa                                                                                                                        <  1:221368/140‑1 (MQ=255)               ttGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAg                                                                                                                >  2:317404/1‑140 (MQ=255)                 gTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCa                                                                                                              <  2:557111/140‑1 (MQ=255)                   cATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACGGCGTAACATTAAGCAGt                                                                                                            <  2:380587/140‑1 (MQ=255)                        aTGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATc                                                                                                       <  1:745186/140‑1 (MQ=255)                            aGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAAt                                                                                                   <  1:394671/140‑1 (MQ=255)                               cGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGt                                                                                                <  1:844686/140‑1 (MQ=255)                                   aTGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTAc                                                                                            >  2:203143/1‑140 (MQ=255)                                   aTGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTAc                                                                                            <  1:317404/140‑1 (MQ=255)                                    tGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTAcc                                                                                           <  1:673211/140‑1 (MQ=255)                                     gTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTAccc                                                                                          <  1:695456/140‑1 (MQ=255)                                        aCTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGt                                                                                       <  1:637079/140‑1 (MQ=255)                                         cTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTa                                                                                      <  2:614896/140‑1 (MQ=255)                                              cGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACAttt                                                                                 >  1:160073/1‑140 (MQ=255)                                              cGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACAttt                                                                                 >  1:413832/1‑140 (MQ=255)                                              cGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACAttt                                                                                 >  1:78498/1‑140 (MQ=255)                                              cGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACAttt                                                                                 >  2:728491/1‑140 (MQ=255)                                                                ccACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGcc                                                                           <  2:367645/128‑1 (MQ=255)                                                                ccACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGcc                                                                           >  1:367645/1‑128 (MQ=255)                                                                  acaTGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAAtgcctgc                                                             >  2:911389/1‑140 (MQ=255)                                                                     tGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTgccgc                                                          >  1:148892/1‑140 (MQ=255)                                                                           aCAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTtgg                                                    >  1:664593/1‑140 (MQ=255)                                                                               tCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGc                                                >  1:128065/1‑140 (MQ=255)                                                                               tCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGc                                                >  1:520413/1‑140 (MQ=255)                                                                               tCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGc                                                >  1:923245/1‑140 (MQ=255)                                                                                ccGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGca                                               >  2:606999/1‑140 (MQ=255)                                                                                    gTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGcaacaa                                           >  1:825880/1‑140 (MQ=255)                                                                                      cAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGt                                         >  2:633074/1‑140 (MQ=255)                                                                                       aGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGtt                                        >  1:714129/1‑140 (MQ=255)                                                                                         tttACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGtttt                                      >  2:236233/1‑140 (MQ=255)                                                                                               gCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAag                                <  1:235567/140‑1 (MQ=255)                                                                                                     cATATCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAagtggagt                          <  1:280279/140‑1 (MQ=255)                                                                                                       tatCGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAagtggagtgg                        >  2:526966/1‑140 (MQ=255)                                                                                                          cGAAGAACGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGtt                     <  2:407317/140‑1 (MQ=255)                                                                                                          cGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGTAACATTAAGCAGTTGATCGCCTTGTTTACCCAGAACATTTAGCGCCAGCGCAATGCCTGCCGCCTTTGGGGGCAACAAATTTTGAAAAGTGGAGTGGGtt                     >  2:774546/1‑140 (MQ=255)                                                                                                            aagaaCGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTg                   <  2:413832/140‑1 (MQ=255)                                                                                                             agaaCGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGc                  >  2:99498/1‑140 (MQ=255)                                                                                                              gaaCGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCt                 <  1:911389/140‑1 (MQ=255)                                                                                                              gaaCGGCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCt                 <  1:728491/140‑1 (MQ=255)                                                                                                                  ggCTGACGGTTATTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATg             >  1:835626/1‑140 (MQ=255)                                                                                                                             aTTGTCCGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCg  >  1:52603/1‑140 (MQ=255)                                                                                                                                  ccGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTg    <  2:588964/133‑1 (MQ=255)                                                                                                                                  ccGGATAGCACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTg    >  1:588964/1‑133 (MQ=255)                                                                                                                                         |                                                                                                                                GGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGTAACATTAAGCAGTTTATCGAATTGTTTACCCAGTACATTTAGCGCCATCGCAATGCCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCG  >  NC_000913/4045756‑4046019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑