Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,081,668	A→G	F65L (TTT→CTT)	fdoI ←	formate dehydrogenase O subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,081,668	0	A	G	100.0%	166.7 / NA	48	F65L (TTT→CTT)	fdoI	formate dehydrogenase O subunit gamma
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/20);  total (28/20)

AAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAACATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATC  >  NC_000913/4081535‑4081804                                                                                                                                      |                                                                                                                                         aaGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAAc                                                                                                                                    <  2:930581/140‑1 (MQ=255)  aGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAAcc                                                                                                                                   <  2:802723/140‑1 (MQ=255)            cccGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACaa                                                                                                                         >  2:303782/1‑140 (MQ=255)             ccGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACaaa                                                                                                                        >  2:144375/1‑140 (MQ=255)               ggTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACg                                                                                                                      <  1:494097/140‑1 (MQ=255)                       cTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGa                                                                                                              >  2:415783/1‑140 (MQ=255)                           ttCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTc                                                                                                          >  2:855396/1‑140 (MQ=255)                           ttCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTc                                                                                                          >  1:535925/1‑140 (MQ=255)                             ccTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTcgc                                                                                                        >  2:77006/1‑140 (MQ=255)                             ccTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTcgc                                                                                                        >  2:21352/1‑140 (MQ=255)                                 gTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCa                                                                                                    >  1:424520/1‑140 (MQ=255)                                    gacgacGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCt                                                                                                 >  2:225584/1‑140 (MQ=255)                                     acgacgATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTg                                                                                                <  2:189176/140‑1 (MQ=255)                                     acgacgATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTg                                                                                                >  1:357727/1‑140 (MQ=255)                                         cgaTCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGctgt                                                                                            <  1:225584/140‑4 (MQ=255)                                         cgaTCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGgt                                                                                            <  1:927222/140‑1 (MQ=255)                                          gaTCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGgtg                                                                                           <  1:144375/140‑1 (MQ=255)                                                      atTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGAttt                                                                               >  2:396694/1‑140 (MQ=255)                                                          ttcGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCAt                                                                           >  2:533733/1‑140 (MQ=255)                                                          ttcGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCAt                                                                           >  2:104735/1‑140 (MQ=255)                                                            cGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATc                                                                          >  2:525355/1‑139 (MQ=255)                                                            cGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATc                                                                          <  1:525355/139‑1 (MQ=255)                                                              cccAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAAc                                                                       <  1:21352/140‑1 (MQ=255)                                                              cccAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAAc                                                                       <  2:357727/140‑1 (MQ=255)                                                              cccAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAAc                                                                       <  1:206775/140‑1 (MQ=255)                                                                  aaaGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGt                                                                   >  2:157776/1‑140 (MQ=255)                                                                         tcatcCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGa                                                            <  2:821049/140‑1 (MQ=255)                                                                             cccGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACggg                                                        >  1:182524/1‑140 (MQ=255)                                                                              ccGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGa                                                       >  1:125332/1‑140 (MQ=255)                                                                               cGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGaa                                                      >  1:377190/1‑140 (MQ=255)                                                                                gattgattAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAAc                                                     >  1:650316/1‑140 (MQ=255)                                                                                          gTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAagccca                                           >  1:3387/1‑140 (MQ=255)                                                                                              tgATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAagcccagccc                                       >  1:915574/1‑140 (MQ=255)                                                                                                   ccAGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCa                                  >  2:101548/1‑140 (MQ=255)                                                                                                     aGTAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCAcc                                <  1:533733/140‑1 (MQ=255)                                                                                                       tAACGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCCCAccgc                              >  1:711725/1‑140 (MQ=255)                                                                                                        aaCGGAAAAACATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCAccgcc                             >  2:93046/1‑140 (MQ=255)                                                                                                                aaCATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGAt                     <  1:413845/140‑1 (MQ=255)                                                                                                                  cATGATGATGAACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGa                   <  1:104735/140‑1 (MQ=255)                                                                                                                    tgatgatgaACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAg                 <  1:855396/140‑1 (MQ=255)                                                                                                                         atgaACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGaa            <  1:157776/140‑1 (MQ=255)                                                                                                                           gaACGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAgg          <  1:303782/140‑1 (MQ=255)                                                                                                                             aCGAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCg        >  2:545397/1‑140 (MQ=255)                                                                                                                               gAGGCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGt      <  1:93046/140‑1 (MQ=255)                                                                                                                                 ggCAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGGTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGa    >  2:677102/1‑140 (MQ=255)                                                                                                                                   cAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATc  <  2:125332/140‑1 (MQ=255)                                                                                                                                   cAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATc  >  2:269217/1‑140 (MQ=255)                                                                                                                                   cAAGCATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATc  >  2:862534/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         AAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCATCCCGATTGATTAGGTTGTGATGCCAGTAACGGAAAAACATGATGATGAACGAGGCAAACATAACCACGCCGACAAACGGGTGCAGAATTCGCGCCAGCTGCGGTGTGCCCATGATTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATC  >  NC_000913/4081535‑4081804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑