| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,195,882 | A→G | V73A (GTC→GCC) | thiC ← | phosphomethylpyrimidine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,195,882 | 0 | A | G | 97.7% | 142.2 / ‑4.1 | 43 | V73A (GTC→GCC) | thiC | phosphomethylpyrimidine synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/22); minor base T (1/0); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
GTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGC > NC_000913/4195747‑4196005 | gtgtAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCgg > 1:679233/1‑140 (MQ=255) aTCGGAACTGCGCACGGCAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGCAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTTCTGATCACCCTACCTGCCGGAGGCGGCGTAGGCCCGAATCg > 2:431428/1‑140 (MQ=255) tCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGc > 1:753053/1‑140 (MQ=255) tCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGc > 2:366358/1‑140 (MQ=255) cGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCt < 2:812382/140‑1 (MQ=255) aCTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGt > 2:375322/1‑140 (MQ=255) aCTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGt < 1:245287/140‑1 (MQ=255) aCTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGt < 2:89415/140‑1 (MQ=255) cgcACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTttc > 2:864519/1‑140 (MQ=255) cgcACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTttc > 2:494944/1‑140 (MQ=255) gcACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTttct > 1:242477/1‑140 (MQ=255) tAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTAc < 1:216571/140‑1 (MQ=255) tAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTAc > 2:216571/1‑140 (MQ=255) aGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTg < 1:67796/140‑1 (MQ=255) tcttcGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCgg < 1:953304/140‑1 (MQ=255) cGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTTGTACTGCGGCTGt > 2:689054/1‑140 (MQ=255) tCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCttt > 2:523865/1‑140 (MQ=255) tCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCt > 1:352204/1‑138 (MQ=255) tCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCt < 2:352204/138‑1 (MQ=255) gccgcGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTTGTACTGCGGCTGTTCTTTGc < 1:689054/140‑1 (MQ=255) cgcgcgCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCTCCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTa < 1:175790/140‑1 (MQ=255) cgcgcgCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTa < 1:494944/140‑1 (MQ=255) cACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGt < 2:941464/140‑1 (MQ=255) gCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCggg < 1:193887/140‑1 (MQ=255) gCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCggg < 1:366358/140‑1 (MQ=255) gCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCt > 2:34995/1‑138 (MQ=255) gCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCt < 1:34995/138‑1 (MQ=255) gCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTaa > 2:113813/1‑140 (MQ=255) gCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTaa > 2:341067/1‑140 (MQ=255) gCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTaa > 1:304706/1‑140 (MQ=255) gCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTaa > 2:17061/1‑140 (MQ=255) gCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGc > 2:512669/1‑140 (MQ=255) aCGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGgcacgcg < 2:768509/140‑1 (MQ=255) tAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGgcacgcgcacg < 1:113813/140‑1 (MQ=255) tGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCg < 1:512669/140‑1 (MQ=255) tGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCg < 2:679233/140‑1 (MQ=255) tGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCg < 2:354906/140‑1 (MQ=255) aaTCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGgt < 2:563644/140‑1 (MQ=255) aTCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGgtg > 1:692802/1‑140 (MQ=255) aTCCCCATACGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAg < 1:431428/140‑1 (MQ=255) cGGGCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATaaa < 1:861345/140‑1 (MQ=255) gggCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAAt > 1:764608/1‑140 (MQ=255) gCCGGAGGTGTCGTAGTCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCTCGCACCGGCACGCGCCCGCCGGGGGGCGTGCCCGAGATATAAAtcc > 2:926001/1‑138 (MQ=255) gCCGGAGGTGTCGTAGGCCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGc > 1:59174/1‑140 (MQ=255) | GTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTCACGCATCGGCACGCGCACGCCGGGGTGTGTGCCAGTGATATAAATGC > NC_000913/4195747‑4196005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |