Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,253,791 A→G N259S (AAC→AGC)  ubiA → 4‑hydroxybenzoate octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,253,7910AG97.1% 233.4 / ‑2.4 70N259S (AAC→AGC) ubiA4‑hydroxybenzoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (33/35);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAACCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTT  >  NC_000913/4253655‑4253924
                                                                                                                                        |                                                                                                                                     
attattGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCcg                                                                                                                                    >  2:811503/1‑140 (MQ=255)
  tattGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCcgcg                                                                                                                                  >  1:47150/1‑140 (MQ=255)
   attGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGTCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGa                                                                                                                                 >  2:979194/1‑140 (MQ=255)
      ggTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCg                                                                                                                              >  1:429153/1‑140 (MQ=255)
             tGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGcc                                                                                                                       <  2:47150/140‑1 (MQ=255)
               cAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTg                                                                                                                     <  1:979194/140‑1 (MQ=255)
                  aTTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCcg                                                                                                                                    >  1:366293/1‑122 (MQ=255)
                  aTTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCcg                                                                                                                                    <  2:366293/122‑1 (MQ=255)
                  aTTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCtt                                                                                                                  >  2:846216/1‑140 (MQ=255)
                    tGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTa                                                                                                                <  2:139842/140‑1 (MQ=255)
                      gCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTa                                                                                                                <  2:787247/138‑1 (MQ=255)
                      gCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTa                                                                                                                >  1:787247/1‑138 (MQ=255)
                      gCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCT‑GGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAg                                                                                                             <  1:571677/140‑1 (MQ=255)
                       cGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAg                                                                                                             <  1:846216/140‑1 (MQ=255)
                       cGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAg                                                                                                             <  2:274971/140‑1 (MQ=255)
                          aCTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCAt                                                                                                          <  1:188539/140‑1 (MQ=255)
                             ggCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTa                                                                                                       <  1:218188/140‑1 (MQ=255)
                             ggCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTa                                                                                                       <  1:384339/140‑1 (MQ=255)
                                    aTGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGaataa                                                                                                >  1:838964/1‑140 (MQ=255)
                                    aTGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGaata                                                                                                 <  1:467904/139‑1 (MQ=255)
                                    aTGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGaata                                                                                                 >  2:467904/1‑139 (MQ=255)
                                    aTGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGaat                                                                                                  <  1:742824/138‑1 (MQ=255)
                                    aTGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGaat                                                                                                  >  2:742824/1‑138 (MQ=255)
                                      ggCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTa                                                                                                       <  1:168860/131‑1 (MQ=255)
                                      ggCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTa                                                                                                       >  2:168860/1‑131 (MQ=255)
                                      ggCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAataata                                                                                              <  1:347909/140‑1 (MQ=255)
                                       gCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAAtaataa                                                                                             <  2:572392/140‑1 (MQ=255)
                                       gCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAAtaataa                                                                                             <  2:474376/140‑1 (MQ=255)
                                        cGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAAc                                                                                            >  2:873613/1‑140 (MQ=255)
                                              tcGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAGAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGtt                                                                                      >  2:859491/1‑140 (MQ=255)
                                                  tGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTc                                                                                  <  1:873613/140‑1 (MQ=255)
                                                      ttAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGt                                                                              >  2:351910/1‑140 (MQ=255)
                                                      ttAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAACCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGt                                                                              >  1:307230/1‑140 (MQ=255)
                                                       tAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTa                                                                             >  1:293543/1‑140 (MQ=255)
                                                         aaTGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATg                                                                                        >  2:504730/1‑127 (MQ=255)
                                                         aaTGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATg                                                                                        <  1:504730/127‑1 (MQ=255)
                                                                tAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTa                                                                    >  1:614939/1‑140 (MQ=255)
                                                                   gCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAACCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAggg                                                                 <  2:307230/140‑1 (MQ=255)
                                                                       gggATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAGAACTGATTGCCAGCCGCGAGCCTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTACTTTTAGGGCTgg                                                             <  1:859491/140‑1 (MQ=255)
                                                                       gggATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTgg                                                             >  2:478471/1‑140 (MQ=255)
                                                                       gggATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTgg                                                             >  1:178789/1‑140 (MQ=255)
                                                                       gggATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTgg                                                             >  2:670169/1‑140 (MQ=255)
                                                                       gggATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTCATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTgg                                                             >  1:163203/1‑140 (MQ=255)
                                                                        ggATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGc                                                            <  2:293543/140‑1 (MQ=255)
                                                                           tatTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAAt                                                         <  1:478471/140‑1 (MQ=255)
                                                                             ttACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGa                                                       >  2:676189/1‑140 (MQ=255)
                                                                                cTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGtt                                                    <  1:811503/140‑1 (MQ=255)
                                                                                  ggTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTAc                                                  >  2:320331/1‑140 (MQ=255)
                                                                                   gTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACt                                                 >  1:507637/1‑140 (MQ=255)
                                                                                   gTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACt                                                 >  2:723140/1‑140 (MQ=255)
                                                                                     cAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTgg                                               <  1:723140/140‑1 (MQ=255)
                                                                                     cAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTgg                                               <  2:838964/140‑1 (MQ=255)
                                                                                      aaTTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGc                                              <  2:178789/140‑1 (MQ=255)
                                                                                        ttCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGTATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCAt                                            >  2:782600/1‑140 (MQ=255)
                                                                                             gtggCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCtg                                       >  1:549814/1‑140 (MQ=255)
                                                                                             gtggCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCtg                                       >  1:66670/1‑140 (MQ=255)
                                                                                              tggctggcGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCtga                                      <  2:66670/140‑1 (MQ=255)
                                                                                                 ctggcGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCtgatga                                   <  1:154773/140‑1 (MQ=255)
                                                                                                  tggcGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACt                                                 >  2:697945/1‑125 (MQ=255)
                                                                                                  tggcGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACt                                                 <  1:697945/125‑1 (MQ=255)
                                                                                                                 ttATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGgat                   <  1:676189/140‑1 (MQ=255)
                                                                                                                 ttATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGgat                   <  2:614939/140‑1 (MQ=255)
                                                                                                                 ttATCAACAAAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGgat                   <  2:507637/140‑1 (MQ=255)
                                                                                                                      aacaaAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGatca              >  1:652839/1‑140 (MQ=255)
                                                                                                                        caaAAACTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGatcatc            >  2:830455/1‑140 (MQ=255)
                                                                                                                             aCTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCt       >  2:918518/1‑140 (MQ=255)
                                                                                                                             aCTGATTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCt       <  1:830455/140‑1 (MQ=255)
                                                                                                                                 aTTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCTttct   <  1:559214/140‑1 (MQ=255)
                                                                                                                                 aTTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCTttct   >  2:559214/1‑140 (MQ=255)
                                                                                                                                 aTTGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCTttct   >  1:47592/1‑140 (MQ=255)
                                                                                                                                  ttGCCAGCCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCTttctt  <  2:652839/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
ATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTGGTCAATTCTGGTGGCTGGCGCGCTGTTTGTTTATCAACAAAAACTGATTGCCAACCGCGAGCGTGAAGCCTGCTTTAAAGCATTTATGAATAATAACTATGTTGGTCTGGTACTATTTTTAGGGCTGGCAATGAGTTACTGGCATTTCTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTT  >  NC_000913/4253655‑4253924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑