Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,304,220	G→A	Q62* (CAG→TAG)	ytcA ←	putative lipoprotein YtcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,304,220	0	G	A	100.0%	285.4 / NA	80	Q62* (CAG→TAG)	ytcA	putative lipoprotein YtcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/34);  total (46/34)

GGAGCTTTTTTCGGCGTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTGAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGACGGGAATTATCC  >  NC_000913/4304084‑4304354                                                                                                                                         |                                                                                                                                       ggagCTTTTTTCGGCGTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAAt                                                                                                                                     >  1:233102/1‑140 (MQ=255)      ttttttCGGCGTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTc                                                                                                                                <  2:194654/140‑1 (MQ=255)         tttCGGCGTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGcc                                                                                                                             <  2:2349/140‑1 (MQ=255)             ggCGTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGt                                                                                                                         >  2:62157/1‑140 (MQ=255)                gTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGtgat                                                                                                                      <  2:847589/140‑1 (MQ=255)                 tACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGtgatg                                                                                                                     <  1:181065/140‑1 (MQ=255)                  aCTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGtgatga                                                                                                                    >  2:164144/1‑140 (MQ=255)                    tttCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGACATTTGCCCGCTAAATAACTCGCCTCGTGATGAGc                                                                                                                  <  2:547870/140‑1 (MQ=255)                             gcgcGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATa                                                                                                         <  1:893066/140‑1 (MQ=255)                               gcgTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAg                                                                                                       <  1:433011/140‑1 (MQ=255)                                cgTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAgg                                                                                                      >  1:537140/1‑140 (MQ=255)                                cgTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAgg                                                                                                      >  1:436900/1‑140 (MQ=255)                                cgTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAG‑GAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGc                                                                                                     >  2:747009/1‑140 (MQ=255)                                  tctcAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCt                                                                                                    >  2:858769/1‑140 (MQ=255)                                             ttAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGgcgc                                                                                         >  1:186349/1‑140 (MQ=255)                                             ttAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGgcgc                                                                                         >  2:334590/1‑140 (MQ=255)                                             ttAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGgcgc                                                                                         >  1:539750/1‑140 (MQ=255)                                              tAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCa                                                                                        >  2:699174/1‑140 (MQ=255)                                              tAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCa                                                                                        <  1:164144/140‑1 (MQ=255)                                                 aaaaaTGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATCGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGaa                                                                                     >  2:905259/1‑140 (MQ=255)                                                       gccagccaGAACAGCATGGCGTAG‑GAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAg                                                                              <  1:747009/140‑1 (MQ=255)                                                        ccagccagAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCTGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAg                                                                              <  2:214262/140‑1 (MQ=255)                                                        ccagccagAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAg                                                                              <  2:436900/140‑1 (MQ=255)                                                          agccagAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCt                                                                            <  1:37843/140‑1 (MQ=255)                                                          agccagAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCt                                                                            >  1:372824/1‑140 (MQ=255)                                                          agccagAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCt                                                                            <  2:233102/140‑1 (MQ=255)                                                          agccagAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCt                                                                            >  1:316221/1‑140 (MQ=255)                                                                     cATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAg                                                                              >  2:723805/1‑127 (MQ=255)                                                                     cATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAg                                                                              <  1:723805/127‑1 (MQ=255)                                                                       tGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAgcg                                                               <  2:537140/140‑1 (MQ=255)                                                                          cGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCg                                                            <  2:372824/140‑1 (MQ=255)                                                                          cGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCg                                                            >  2:384368/1‑140 (MQ=255)                                                                          cGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCg                                                            >  1:273875/1‑140 (MQ=255)                                                                                  gCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCgg                                                    >  1:796365/1‑140 (MQ=255)                                                                                   cAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCggg                                                   >  1:803366/1‑140 (MQ=255)                                                                                   cAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCggg                                                   >  1:820759/1‑140 (MQ=255)                                                                                   cAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCggg                                                   >  2:738851/1‑140 (MQ=255)                                                                                   cAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCggg                                                   >  1:129785/1‑140 (MQ=255)                                                                                    aaaaaGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  <  2:946967/140‑1 (MQ=255)                                                                                    aaaaaGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  >  1:946967/1‑140 (MQ=255)                                                                                    aaaaaGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  <  1:222205/140‑1 (MQ=255)                                                                                    aaaaaGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  >  1:378189/1‑140 (MQ=255)                                                                                    aaaaaGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  >  1:150497/1‑140 (MQ=255)                                                                                      aaaGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGAtt                                                >  2:516344/1‑140 (MQ=255)                                                                                        aGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGc                                              <  1:858769/140‑1 (MQ=255)                                                                                          ggCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCg                                            <  2:287684/140‑1 (MQ=255)                                                                                           gCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCgg                                           <  1:699174/140‑1 (MQ=255)                                                                                            cGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGga                                          <  1:62157/140‑1 (MQ=255)                                                                                              gTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  <  2:887683/130‑1 (MQ=255)                                                                                              gTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGa                                                  >  1:887683/1‑130 (MQ=255)                                                                                               tataAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGAt                                       >  2:118251/1‑140 (MQ=255)                                                                                               tataAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGAt                                       <  2:150497/140‑1 (MQ=255)                                                                                                    ataatTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGgag                                         <  1:44816/133‑1 (MQ=255)                                                                                                    ataatTCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGgag                                         >  2:44816/1‑133 (MQ=255)                                                                                                        ttCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCAt                              >  1:224782/1‑140 (MQ=255)                                                                                                        ttCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCAt                              >  1:933696/1‑140 (MQ=255)                                                                                                         tCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATc                             >  2:723981/1‑140 (MQ=255)                                                                                                         tCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATc                             >  2:659193/1‑140 (MQ=255)                                                                                                         tCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATc                             >  1:685212/1‑140 (MQ=255)                                                                                                         tCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATc                             >  1:628816/1‑140 (MQ=255)                                                                                                         tCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATc                             >  2:840873/1‑140 (MQ=255)                                                                                                         tCCGACAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATc                             >  1:554580/1‑140 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCgg                                           <  2:462933/122‑1 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCgg                                           >  1:462933/1‑122 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGa                         <  2:224782/140‑1 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGa                         >  2:267615/1‑140 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGa                         >  2:198435/1‑140 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGa                         >  1:437506/1‑140 (MQ=255)                                                                                                             aCAAATGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCAGGAGATAATGAGCATCCCGa                         >  1:589224/1‑140 (MQ=255)                                                                                                              cAAATGCCAGATCGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGGGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGaa                        <  1:905259/140‑1 (MQ=255)                                                                                                               aaaTGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGaaa                       <  2:528384/140‑1 (MQ=255)                                                                                                                 aTGCCAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAAcc                     <  2:129785/140‑1 (MQ=255)                                                                                                                    ccAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGTAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATg                  <  2:685212/140‑1 (MQ=255)                                                                                                                    ccAGATTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATg                  <  1:497561/140‑1 (MQ=255)                                                                                                                        aTTGATATTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTC‑AAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGAc             >  2:489822/1‑140 (MQ=255)                                                                                                                            atatTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGACggg          >  2:348171/1‑140 (MQ=255)                                                                                                                              atTTGCCCGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGACGGGaa        <  2:186349/140‑1 (MQ=255)                                                                                                                                   cccGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGAc             >  1:114801/1‑130 (MQ=255)                                                                                                                                   cccGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGAc             <  2:114801/130‑1 (MQ=255)                                                                                                                                    ccGCTAAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGACGGGAATTATcc  >  1:390087/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGAGCTTTTTTCGGCGTACTTTCCATGAGCGCGTCTCAAATAGATTAGAAAAATGCCAGCCAGAACAGCATGGCGTAGAGAGCAAAAAGGGCGGTATAAATAATTCCGACAAATGCCAGATTGATATTTGCCCGCTGAATAACTCGCCTCGTGATGAGCGTCAAAATAAGGCTGGCAATGGCGCAGAAAAACCAGCTGGGATAATAAGCGCCGATCACCGGGATTGCCGGAGATAATGAGCATCCCGAAACCATGAAGACGGGAATTATCC  >  NC_000913/4304084‑4304354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑