Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,409,607	G→A	A111A (GCG→GCA)	rlmB →	23S rRNA 2'‑O‑ribose G2251 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,409,607	0	G	A	100.0%	160.9 / NA	46	A111A (GCG→GCA)	rlmB	23S rRNA 2'‑O‑ribose G2251 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/22);  total (24/22)

GTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAAC  >  NC_000913/4409470‑4409742                                                                                                                                          |                                                                                                                                        gTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATg                                                                                                                                       >  1:807795/1‑140 (MQ=255)  tGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGGTCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGc                                                                                                                                      <  1:637313/140‑1 (MQ=255)  tGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACTGATCCGCACAACCTCGGCGCATGc                                                                                                                                      <  1:649444/140‑1 (MQ=255)       cAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCg                                                                                                                                 >  2:225889/1‑140 (MQ=255)                       gcgTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCa                                                                                                                 <  2:308371/140‑1 (MQ=255)                        cgTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAg                                                                                                                >  2:554435/1‑140 (MQ=255)                             aGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGtt                                                                                                           <  1:789838/140‑1 (MQ=255)                               ccAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCa                                                                                                         <  1:877742/140‑1 (MQ=255)                                      gTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTg                                                                                                  >  2:725968/1‑140 (MQ=255)                                               aGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCg                                                                                         >  2:216811/1‑140 (MQ=255)                                               aGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCg                                                                                         <  1:8030/140‑1 (MQ=255)                                                ggAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGa                                                                                        >  2:87111/1‑140 (MQ=255)                                                    aaCGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGa                                                                                    <  1:554435/140‑1 (MQ=255)                                                    aaCGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGa                                                                                    <  2:111162/140‑1 (MQ=255)                                                         tCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCt                                                                               <  1:675789/140‑1 (MQ=255)                                                         tCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCt                                                                               <  1:225889/140‑1 (MQ=255)                                                           tGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTcc                                                                             >  1:883284/1‑140 (MQ=255)                                                             ccGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGc                                                                           >  1:389275/1‑140 (MQ=255)                                                                gATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGcaca                                                                        >  2:899876/1‑140 (MQ=255)                                                                       tcgcttcgctCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                                                          <  2:775226/131‑1 (MQ=255)                                                                       tcgcttcgctCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                                                          >  1:775226/1‑131 (MQ=255)                                                                           ttcgctCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTa                                                             >  2:407440/1‑140 (MQ=255)                                                                                tCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                                                          <  2:845571/122‑1 (MQ=255)                                                                                tCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                                                          >  1:845571/1‑122 (MQ=255)                                                                                       aCCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAg                                                 <  1:725968/140‑1 (MQ=255)                                                                                       aCCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAg                                                 >  1:667570/1‑140 (MQ=255)                                                                                        ccGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACg                                                           <  2:651417/129‑1 (MQ=255)                                                                                        ccGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACg                                                           >  1:651417/1‑129 (MQ=255)                                                                                            tCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACg                                                           <  2:848311/125‑1 (MQ=255)                                                                                            tCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACg                                                           >  1:848311/1‑125 (MQ=255)                                                                                            tCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGcc                                            >  1:182398/1‑140 (MQ=255)                                                                                             ccTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCt                                           >  1:548364/1‑140 (MQ=255)                                                                                                   gATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTgcggcg                                     <  2:434707/140‑1 (MQ=255)                                                                                                         cGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAg                               >  1:578196/1‑140 (MQ=255)                                                                                                         cGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAg                               >  2:737253/1‑140 (MQ=255)                                                                                                         cGACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAg                               >  2:808894/1‑140 (MQ=255)                                                                                                          gACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGa                              >  1:490038/1‑140 (MQ=255)                                                                                                          gACGGCGTAACCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGa                              <  1:87111/140‑1 (MQ=255)                                                                                                                   aCCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTcc                     <  1:737253/140‑1 (MQ=255)                                                                                                                   aCCGATCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTcc                     <  1:808894/140‑1 (MQ=255)                                                                                                                        tCCGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGa                <  1:369180/140‑1 (MQ=255)                                                                                                                         ccGCACAACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCCGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGAt               >  1:967821/1‑140 (MQ=255)                                                                                                                            cacaACCTCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCg            >  1:640740/1‑140 (MQ=255)                                                                                                                                   tCGGCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACt     <  1:216811/140‑1 (MQ=255)                                                                                                                                     ggCGCATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTaa   >  1:531716/1‑140 (MQ=255)                                                                                                                                      gcgcATGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAAc  <  2:490038/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAAC  >  NC_000913/4409470‑4409742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑