Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,321	A→G	V506A (GTA→GCA)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,321	0	A	G	100.0%	239.0 / NA	67	V506A (GTA→GCA)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/34);  total (33/34)

TTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGCAGGACAACATCAGCACC  >  NC_000913/4482185‑4482457                                                                                                                                         |                                                                                                                                         ttCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCa                                                                                                                                       >  1:309868/1‑140 (MQ=255)  tCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCAt                                                                                                                                      <  1:146207/140‑1 (MQ=255)  tCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCAt                                                                                                                                      >  1:100534/1‑140 (MQ=255)  tCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCAt                                                                                                                                      <  1:269228/140‑1 (MQ=255)    gTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCa                                                                                                                                    >  1:893743/1‑140 (MQ=255)      catcaCGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACg                                                                                                                                  <  2:100534/140‑1 (MQ=255)        tcaCGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCt                                                                                                                                >  2:451857/1‑140 (MQ=255)          aCGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTgg                                                                                                                              <  2:368848/140‑1 (MQ=255)          aCGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTgg                                                                                                                              <  1:927039/140‑1 (MQ=255)                   gCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGa                                                                                                                     >  2:484874/1‑140 (MQ=255)                   gCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCACGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGa                                                                                                                     >  1:554288/1‑140 (MQ=255)                      ggTCATGTAAACGGTGTGGAACGGCACCTGCGGGTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACt                                                                                                                  >  1:946279/1‑140 (MQ=255)                       gTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGt                                                                                                                        >  1:239760/1‑133 (MQ=255)                       gTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGt                                                                                                                        <  2:239760/133‑1 (MQ=255)                        tCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGa                                                                                                                >  1:562385/1‑140 (MQ=255)                           tGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTgg                                                                                                                      >  1:902924/1‑131 (MQ=255)                           tGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTgg                                                                                                                      <  2:902924/131‑1 (MQ=255)                                 cGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCg                                                                                                       >  1:337641/1‑140 (MQ=255)                                 cGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCg                                                                                                       <  1:660488/140‑1 (MQ=255)                                  ggTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGt                                                                                                      >  1:328968/1‑140 (MQ=255)                                     gtgGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCgg                                                                                                   >  1:807654/1‑140 (MQ=255)                                           cGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTAtttt                                                                                             >  2:557386/1‑140 (MQ=255)                                              cACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCg                                                                                          >  1:701535/1‑140 (MQ=255)                                                ccTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgc                                                                                        >  1:416577/1‑140 (MQ=255)                                                ccTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgc                                                                                        <  2:665776/140‑1 (MQ=255)                                                     ggTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgccagcc                                                                                   <  1:557386/140‑1 (MQ=255)                                                     ggTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgccagcc                                                                                   <  1:26945/140‑1 (MQ=255)                                                     ggTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgccagcc                                                                                   <  2:309868/140‑1 (MQ=255)                                                      gTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgccagcc                                                                                   >  2:90495/1‑139 (MQ=255)                                                      gTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgccagcc                                                                                   <  1:90495/139‑1 (MQ=255)                                                      gTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCACGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGgccagcca                                                                                  <  2:554288/140‑1 (MQ=255)                                                        ttGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAg                                                                                <  2:246166/140‑1 (MQ=255)                                                           ccATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGt                                                                             >  1:191561/1‑140 (MQ=255)                                                           ccATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGt                                                                             <  2:328968/140‑1 (MQ=255)                                                                ttCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAgaga                                                                        >  1:942288/1‑140 (MQ=255)                                                                  cATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAg                                                                      <  2:701535/140‑1 (MQ=255)                                                                     cTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTc                                                                   <  1:246971/140‑1 (MQ=255)                                                                       ttGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCca                                                                 <  2:191561/140‑1 (MQ=255)                                                                            gaAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAgcg                                                            <  1:484874/140‑1 (MQ=255)                                                                            gaAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAgcg                                                            <  1:571440/140‑1 (MQ=255)                                                                                       tcatcaTGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAAc                                                 >  2:919676/1‑140 (MQ=255)                                                                                       tcatcaTGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAAc                                                 >  2:838982/1‑140 (MQ=255)                                                                                          tcatgatcatgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAg                                              >  1:208195/1‑140 (MQ=255)                                                                                             tgatcatgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTa                                           <  2:807654/140‑1 (MQ=255)                                                                                                 catgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAAc                                                 <  1:792633/130‑1 (MQ=255)                                                                                                 catgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAAc                                                 >  2:792633/1‑130 (MQ=255)                                                                                                 catgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCga                                       >  2:810775/1‑140 (MQ=255)                                                                                                 catgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCga                                       >  2:421810/1‑140 (MQ=255)                                                                                                 catgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCga                                       >  1:325582/1‑140 (MQ=255)                                                                                                 catgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCga                                       >  1:793869/1‑140 (MQ=255)                                                                                                   tgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCgaga                                     >  2:216718/1‑140 (MQ=255)                                                                                                   tgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCgaga                                     <  1:404783/140‑1 (MQ=255)                                                                                                   tgCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCgaga                                     >  2:831329/1‑140 (MQ=255)                                                                                                   tgCGGGCAATCCAGAAGAAAATGATGACGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCgaga                                     >  2:169601/1‑140 (MQ=255)                                                                                                    gCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGaa                                    <  1:831329/140‑1 (MQ=255)                                                                                                       ggCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGt                                 >  2:350965/1‑140 (MQ=255)                                                                                                        gCAATCCAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTc                                >  1:308595/1‑140 (MQ=255)                                                                                                             ccAGAAGAAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGt                           <  1:838982/140‑1 (MQ=255)                                                                                                               agaagaAAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTcc                         <  1:216718/140‑1 (MQ=255)                                                                                                               agaagaAAATGATGACGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTcc                         <  1:169601/140‑1 (MQ=255)                                                                                                                  agaaAATGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGa                      <  2:562385/140‑1 (MQ=255)                                                                                                                    aaaaTGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACg                    <  2:893743/140‑1 (MQ=255)                                                                                                                     aaaTGATGTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGc                   <  1:937582/140‑1 (MQ=255)                                                                                                                          atgTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGCAGGac              <  1:810775/140‑1 (MQ=255)                                                                                                                          atgTCGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGCAGGac              <  1:451857/140‑1 (MQ=255)                                                                                                                              cGAAACCAGATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGCAGGacaaca          >  1:24663/1‑140 (MQ=255)                                                                                                                                      gATGCCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGCAGGACAACATCAGCAcc  <  1:919676/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGATGAAGTGCATGGTCATCATGATCATGCGGGCAATCCAGAAGAAAATGATGTCGAAACCAGATACCATCACGCTGGTTGGGTGGAACTGACGCAGGGCGTCGGTATTTTCCGGCCAGCCAAGGGTAGAGAAGGTCCACAGCGCAGAAGAGAACCAGGTATCGAGAACGTCTTCGTCCTGACGCAGGACAACATCAGCACC  >  NC_000913/4482185‑4482457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑