Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	605,175	A→G	F84L (TTC→CTC)	nfsB ←	NAD(P)H nitroreductase NfsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	605,175	0	A	G	100.0%	210.4 / NA	60	F84L (TTC→CTC)	nfsB	NAD(P)H nitroreductase NfsB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/34);  total (26/34)

TGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGA  >  NC_000913/605042‑605310                                                                                                                                      |                                                                                                                                        tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGcaccac                                                                                                                                   <  2:29747/140‑1 (MQ=255) tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGcaccac                                                                                                                                   >  1:723672/1‑140 (MQ=255) tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGcaccac                                                                                                                                   <  1:385776/140‑1 (MQ=255) tGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGcaccac                                                                                                                                   >  2:899630/1‑140 (MQ=255)   cATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCacga                                                                                                                                 >  1:117175/1‑140 (MQ=255)   cATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCacga                                                                                                                                 >  1:300140/1‑140 (MQ=255)    atatCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTCTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTGCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCacgac                                                                                                                                <  2:673612/140‑1 (MQ=255)       tCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCATGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTg                                                                                                                             <  1:3476/140‑1 (MQ=255)         aGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCg                                                                                                                           <  1:273445/140‑1 (MQ=255)         aGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCg                                                                                                                           <  1:224374/140‑1 (MQ=255)           cGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAg                                                                                                                         >  2:591789/1‑140 (MQ=255)           cGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAg                                                                                                                         >  2:920444/1‑140 (MQ=255)           cGAAGAACTTGCGACCTTTATCGGTCGCGGCTTTCGCTTCCGGGGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAg                                                                                                                         >  2:715173/1‑140 (MQ=255)            gaagaaCTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAgg                                                                                                                        <  1:591789/140‑1 (MQ=255)               gaaCTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCAt                                                                                                                     <  2:949746/140‑1 (MQ=255)                  cTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCaa                                                                                                                  >  2:840600/1‑140 (MQ=255)                   ttGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAg                                                                                                                 <  1:70927/140‑1 (MQ=255)                    tGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGc                                                                                                                >  2:899322/1‑140 (MQ=255)                          cTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGTACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTa                                                                                                          <  1:888960/140‑1 (MQ=255)                           tttATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTTCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTAc                                                                                                         <  1:524322/140‑1 (MQ=255)                             tATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGc                                                                                                       <  2:491488/140‑1 (MQ=255)                                    cgcgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGa                                                                                                >  2:114112/1‑140 (MQ=255)                                     gcgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGaa                                                                                               <  2:723672/140‑1 (MQ=255)                                     gcgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGaa                                                                                               <  2:875439/140‑1 (MQ=255)                                     gcgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGaa                                                                                               <  1:913328/140‑1 (MQ=255)                                     gcgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGaa                                                                                               <  2:300140/140‑1 (MQ=255)                                      cgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAac                                                                                              <  1:920444/140‑1 (MQ=255)                                      cgGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAac                                                                                              <  2:349671/140‑1 (MQ=255)                                       ggCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAaca                                                                                             <  1:346924/140‑1 (MQ=255)                                                   cGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACg                                                                                           >  1:69201/1‑130 (MQ=255)                                                   cGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACg                                                                                           <  2:69201/130‑1 (MQ=255)                                                        tGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCg                                                                            >  2:827826/1‑140 (MQ=255)                                                                     aTCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAacac                                                               >  2:314957/1‑140 (MQ=255)                                                                     aTCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAacac                                                               >  2:977926/1‑140 (MQ=255)                                                                     aTCGGCATCTTCCTGGTCAACAACCACCTTCACCCAGACATCGTCCATCGCGGTTTTTGCCCAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAacac                                                               >  2:949633/1‑140 (MQ=255)                                                                             cTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTa                                                       >  2:248175/1‑140 (MQ=255)                                                                               tCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTAcc                                                     <  2:617896/140‑1 (MQ=255)                                                                                 cTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACctt                                                   <  2:252328/140‑1 (MQ=255)                                                                                         caacCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTg                                           <  1:114112/140‑1 (MQ=255)                                                                                         caacCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTg                                           >  2:504627/1‑140 (MQ=255)                                                                                          aacCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGc                                          >  2:35908/1‑140 (MQ=255)                                                                                           acCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCt                                         <  2:250459/140‑1 (MQ=255)                                                                                           acCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCt                                         <  1:298333/140‑1 (MQ=255)                                                                                             cAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTgg                                       <  1:840600/140‑1 (MQ=255)                                                                                             cAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTgg                                       <  1:289329/140‑1 (MQ=255)                                                                                                      ccAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATaa                              <  1:899630/140‑1 (MQ=255)                                                                                                       cAGACATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATaaa                             >  1:653890/1‑140 (MQ=255)                                                                                                          aCATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATg                          >  2:703266/1‑140 (MQ=255)                                                                                                          aCATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATg                          >  2:941031/1‑140 (MQ=255)                                                                                                           cATCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGc                         >  2:961093/1‑140 (MQ=255)                                                                                                            aTCGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGcc                        <  1:703266/140‑1 (MQ=255)                                                                                                              cGTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCAc                      >  1:957420/1‑140 (MQ=255)                                                                                                               gTCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAGCACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACg                     >  1:223551/1‑140 (MQ=255)                                                                                                                tCCATCGCGGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACgg                    <  2:568871/140‑1 (MQ=255)                                                                                                                     cgcgGTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTggg               >  1:191621/1‑140 (MQ=255)                                                                                                                        ggTTTTTGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGt            <  2:191621/140‑1 (MQ=255)                                                                                                                            tttGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGt        <  1:35908/140‑1 (MQ=255)                                                                                                                              tGCACAGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGc      >  2:884204/1‑140 (MQ=255)                                                                                                                                  caGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGa  <  1:504627/140‑1 (MQ=255)                                                                                                                                  caGAGCACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGa  <  1:527067/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCACAGAACACCACGACGTGCGAGGCATCAAGCATTTTACGCTCGTTGAACACGTAATTACCGGCAGCGGATTTGGCAACACGCGCTTTACCTTCTTCCGTGCTGGCAACAATAAAATGCCACGGCTGGGAGTTGGTGCTGGA  >  NC_000913/605042‑605310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑