Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	627,340	A→G	Y424C (TAC→TGC)	entE →	2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	627,340	0	A	G	100.0%	182.3 / NA	52	Y424C (TAC→TGC)	entE	2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

GAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGA  >  NC_000913/627204‑627472                                                                                                                                         |                                                                                                                                     gAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCAt                                                                                                                                   <  2:209143/140‑1 (MQ=255) gAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCAt                                                                                                                                   <  1:336760/140‑1 (MQ=255)   aGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCa                                                                                                                                 <  1:781057/140‑1 (MQ=255)   aGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCa                                                                                                                                 <  2:95770/140‑1 (MQ=255)        gACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTg                                                                                                                            >  1:644859/1‑140 (MQ=255)          cGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCa                                                                                                                          >  2:517521/1‑140 (MQ=255)              tgatgaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAgggg                                                                                                                      <  2:591402/140‑1 (MQ=255)                    cgcgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGGGGGTTGCATCACCGTGCAGGGGCGCgag                                                                                                                <  1:391047/140‑1 (MQ=255)                    cgcgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCgag                                                                                                                <  1:517521/140‑1 (MQ=255)                     gcgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCgaga                                                                                                               <  1:850450/140‑1 (MQ=255)                      cgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTTCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGaa                                                                                                              >  2:205483/1‑140 (MQ=255)                      cgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGt                                                                                                                             >  2:921929/1‑125 (MQ=255)                      cgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGt                                                                                                                             <  1:921929/125‑1 (MQ=255)                       gcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGaaa                                                                                                             >  1:442732/1‑140 (MQ=255)                        cgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAg                                                                                                            >  1:555346/1‑140 (MQ=255)                         gggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGa                                                                                                           <  2:555346/140‑1 (MQ=255)                              gTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGa                                                                                                      <  2:608565/140‑1 (MQ=255)                                   ccTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAAc                                                                                                 >  2:467949/1‑140 (MQ=255)                                     ttCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCg                                                                                               >  2:215454/1‑140 (MQ=255)                                                ttACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgaga                                                                                    >  2:702714/1‑140 (MQ=255)                                                    aaaaGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGAt                                                                                <  2:427493/140‑1 (MQ=255)                                                    aaaaGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGAt                                                                                <  2:404990/140‑1 (MQ=255)                                                        gTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCt                                                                            >  2:960383/1‑140 (MQ=255)                                                          ccACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGc                                                                          >  1:356853/1‑140 (MQ=255)                                                           cacaGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGcc                                                                         >  1:97551/1‑140 (MQ=255)                                                                cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAaga                                                                    <  1:125168/140‑1 (MQ=255)                                                                cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAaga                                                                    >  1:872129/1‑140 (MQ=255)                                                                cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAaga                                                                    <  1:313399/140‑1 (MQ=255)                                                                   aaTGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGAt                                                                 <  1:828570/140‑1 (MQ=255)                                                                          gcgcCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAAc                                                          >  1:189839/1‑140 (MQ=255)                                                                                          cGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCAcc                                          >  1:234238/1‑140 (MQ=255)                                                                                               tttACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCcggcg                                     <  2:189839/140‑1 (MQ=255)                                                                                                    tGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGAt                                <  2:188744/140‑1 (MQ=255)                                                                                                     gTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATc                               >  2:110123/1‑140 (MQ=255)                                                                                                     gTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATc                               >  1:786395/1‑140 (MQ=255)                                                                                                      ttCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCt                              >  1:750038/1‑140 (MQ=255)                                                                                                       tCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCcggcg                                     >  2:198167/1‑132 (MQ=255)                                                                                                       tCCGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCcggcg                                     <  1:198167/132‑1 (MQ=255)                                                                                                         cGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGTGCCACCCGGCGGTGATCTAcg                           <  1:262215/140‑1 (MQ=255)                                                                                                         cGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcg                           <  2:97551/140‑1 (MQ=255)                                                                                                         cGGCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcg                           <  1:479339/140‑1 (MQ=255)                                                                                                           gCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcgcc                         >  2:426876/1‑140 (MQ=255)                                                                                                           gCGATCTGATCTCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTAcgcc                         >  2:840597/1‑140 (MQ=255)                                                                                                                 tgatctCTATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTg                   <  1:791757/140‑1 (MQ=255)                                                                                                                     ctctATTGATCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGa               >  2:596533/1‑140 (MQ=255)                                                                                                                             aTCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATTAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGaa       >  2:170955/1‑140 (MQ=255)                                                                                                                              tCCAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATTAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAg      <  1:170955/140‑1 (MQ=255)                                                                                                                               ccAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGa     >  2:287106/1‑140 (MQ=255)                                                                                                                                cAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGAt    <  1:808807/140‑1 (MQ=255)                                                                                                                                cAGAGGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGAt    >  1:632255/1‑140 (MQ=255)                                                                                                                                  gagGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGGAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGa  >  2:880365/1‑140 (MQ=255)                                                                                                                                  gagGGTTGCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGa  >  2:524027/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGA  >  NC_000913/627204‑627472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑