Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,873	T→C	R28R (CGT→CGC)	chiQ →	lipoprotein ChiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,873	0	T	C	100.0%	197.6 / NA	57	R28R (CGT→CGC)	chiQ	lipoprotein ChiQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/32);  total (25/32)

TCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGA  >  NC_000913/709736‑709998                                                                                                                                          |                                                                                                                              tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCt                                                                                                                             >  2:607175/1‑140 (MQ=255) tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCt                                                                                                                             >  2:233235/1‑140 (MQ=255)     aTGCCCGACGGGGCAGGTTTTGGGCGTGCGCCAGGTTTGAGGAATTTGCTAGGAAAAACCGGATACACATCGCCATAAGGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCAAAGGAAGACAGTCGCCTGaca                                                                                                                         <  2:170874/140‑3 (MQ=255)     aTGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGaaa                                                                                                                         <  1:224049/140‑1 (MQ=255)     aTGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGaaa                                                                                                                         >  1:714018/1‑140 (MQ=255)      tGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAag                                                                                                                        >  2:270611/1‑140 (MQ=255)      tGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAag                                                                                                                        >  1:438690/1‑140 (MQ=255)        cccGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAagag                                                                                                                      >  2:526079/1‑140 (MQ=255)         ccGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTGGCCTGAAAGAgg                                                                                                                     >  2:454772/1‑140 (MQ=255)         ccGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGACTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAgg                                                                                                                     <  2:507514/140‑1 (MQ=255)          cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGc                                                                                                                    >  1:151397/1‑140 (MQ=255)          cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGc                                                                                                                    >  2:6810/1‑140 (MQ=255)            aCGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCAt                                                                                                                  >  1:147322/1‑140 (MQ=255)             cgcgGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGATAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATa                                                                                                                 >  1:874716/1‑140 (MQ=255)               cgGCCGGTTTTGTGCCTGCCGAAAGTGTGAGGAATTTGCTATGAAAAAGCAGATTCTTATCGCCATAATGGCACAGGGGCTGGTGGCTTGTGCGCAATCACCCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACa                                                                                                               <  2:312442/140‑1 (MQ=255)                  cAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACGgcg                                                                                                            <  2:93998/140‑1 (MQ=255)                  cAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAgcg                                                                                                            <  2:61296/140‑1 (MQ=255)                    ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGcc                                                                                                          <  2:6496/140‑1 (MQ=255)                    ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGcc                                                                                                          <  2:147322/140‑1 (MQ=255)                         tgtgCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTAt                                                                                                     <  1:29287/140‑1 (MQ=255)                            gcctgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCaa                                                                                                  <  1:241646/140‑1 (MQ=255)                              ctgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAaca                                                                                                <  2:707893/140‑1 (MQ=255)                              ctgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAaca                                                                                                <  2:373134/140‑1 (MQ=255)                              ctgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAaca                                                                                                <  1:384215/140‑1 (MQ=255)                                         ttGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCATGAAAGAGGCATACAGCGCCTGTATCAACACCGca                                                                                           <  1:47199/134‑1 (MQ=255)                                         ttGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCATGAAAGAGGCATACAGCGCCTGTATCAACACCGca                                                                                           >  2:47199/1‑134 (MQ=255)                                            aGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCg                                                                                  <  2:119614/140‑1 (MQ=255)                                                      tATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGaaaaaa                                                                        >  1:52664/1‑140 (MQ=255)                                                           aaaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTGGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa                                                                   <  1:454772/140‑1 (MQ=255)                                                           aaaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa                                                                   <  1:526079/140‑1 (MQ=255)                                                           aaaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa                                                                   <  2:438690/140‑1 (MQ=255)                                                            aaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAg                                                                  <  1:270611/140‑1 (MQ=255)                                                                     tctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACATGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                         <  2:401113/140‑1 (MQ=255)                                                                     tctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAATACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                         <  2:149782/140‑1 (MQ=255)                                                                      ctcATCGCCATAGTGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                        <  2:686531/140‑1 (MQ=255)                                                                      ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                        <  1:99620/140‑1 (MQ=255)                                                                         aTCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCag                                                          >  2:727717/1‑135 (MQ=255)                                                                         aTCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCag                                                          <  1:727717/135‑1 (MQ=255)                                                                           cGCCATAATAGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCgtgt                                                   >  2:406901/1‑140 (MQ=255)                                                                             ccATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTa                                                 >  1:955174/1‑140 (MQ=255)                                                                                  aTGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGt                                            >  2:256613/1‑140 (MQ=255)                                                                                   tGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTg                                           <  1:607175/140‑1 (MQ=255)                                                                                        tCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaa                                      >  2:390575/1‑140 (MQ=255)                                                                                        tCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaa                                      >  2:519435/1‑140 (MQ=255)                                                                                          ggggCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaaga                                    <  1:390575/140‑1 (MQ=255)                                                                                            ggCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaa                                  >  2:753987/1‑140 (MQ=255)                                                                                               tGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag                               >  1:954949/1‑140 (MQ=255)                                                                                                 gTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACTGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGaa                             <  2:321113/140‑1 (MQ=255)                                                                                                 gTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGaa                             <  2:52664/140‑1 (MQ=255)                                                                                                   aGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAaac                           <  2:954949/140‑1 (MQ=255)                                                                                                        gtgCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACAt                      >  2:847978/1‑140 (MQ=255)                                                                                                        gtgCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACAt                      <  1:586346/140‑1 (MQ=255)                                                                                                         tgCGCAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAGCATc                     >  2:906288/1‑140 (MQ=255)                                                                                                           cgcAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcag                   >  1:177725/1‑140 (MQ=255)                                                                                                             cAATCAACCGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATcagca                 <  1:753987/140‑1 (MQ=255)                                                                                                                    ccGCGCCACAGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGcc          >  2:540240/1‑140 (MQ=255)                                                                                                                            caGGAAGACAGTCGCCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGa  <  2:151397/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              TCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGA  >  NC_000913/709736‑709998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑