Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	139,481	A→G	V582A (GTC→GCC)	gcd ←	quinoprotein glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	139,481	0	A	G	100.0%	174.7 / NA	50	V582A (GTC→GCC)	gcd	quinoprotein glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

CCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTTCGTCGG  >  NC_000913/139347‑139611                                                                                                                                       |                                                                                                                                   ccATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGc                                                                                                                               <  2:873247/140‑1 (MQ=255) ccATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTAGAACATCCCCAGGTTACCCGGGAAGGCCAGc                                                                                                                               <  1:170357/140‑1 (MQ=255)    tCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTa                                                                                                                            <  2:593685/140‑1 (MQ=255)     cGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTAc                                                                                                                           <  2:370647/140‑1 (MQ=255)       ggTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTAccc                                                                                                                         <  2:328116/140‑1 (MQ=255)            ccAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTc                                                                                                                    >  2:218541/1‑140 (MQ=255)                    aCGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCg                                                                                                            <  1:230006/140‑1 (MQ=255)                     cgcgGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCgg                                                                                                           >  2:641556/1‑140 (MQ=255)                     cgcgGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCgg                                                                                                           >  1:715620/1‑140 (MQ=255)                      gcgGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCggg                                                                                                          >  1:495039/1‑140 (MQ=255)                          gATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGCTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGa                                                                                                      >  1:387140/1‑140 (MQ=255)                             cAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGaaaa                                                                                                   <  1:785411/140‑1 (MQ=255)                                       acaaacGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATa                                                                                         <  2:33201/140‑1 (MQ=255)                                              gCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATc                                                                                  <  1:885719/140‑1 (MQ=255)                                               cAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCa                                                                                           <  2:760722/130‑1 (MQ=255)                                               cAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCa                                                                                           >  1:760722/1‑130 (MQ=255)                                                 gTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCtgg                                                                               <  2:189881/140‑1 (MQ=255)                                                   gCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATc                                                                                  >  2:674977/1‑135 (MQ=255)                                                   gCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATc                                                                                  <  1:674977/135‑1 (MQ=255)                                                   gCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCtggtg                                                                             >  2:644789/1‑140 (MQ=255)                                                   gCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCtggtg                                                                             >  1:654038/1‑140 (MQ=255)                                                      aTTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGaa                                                                          <  2:493552/140‑1 (MQ=255)                                                      aTTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGaa                                                                          <  2:836991/140‑1 (MQ=255)                                                            ttGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAgcgc                                                                                     <  2:354491/123‑1 (MQ=255)                                                            ttGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAgcgc                                                                                     >  1:354491/1‑123 (MQ=255)                                                             tGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCAcg                                                                   <  2:517250/140‑1 (MQ=255)                                                              ggCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCAcgc                                                                  <  1:644789/140‑1 (MQ=255)                                                              ggCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCAcgc                                                                  >  1:625279/1‑140 (MQ=255)                                                                    cGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGcaca                                                            >  2:920886/1‑140 (MQ=255)                                                                     gCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCacac                                                           <  2:715620/140‑1 (MQ=255)                                                                                      aTCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACAtgg                                          >  2:125028/1‑140 (MQ=255)                                                                                        cAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACAtggtg                                        <  1:218541/140‑1 (MQ=255)                                                                                           cGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCt                                     <  1:641556/140‑1 (MQ=255)                                                                                           cGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCt                                     >  1:755780/1‑140 (MQ=255)                                                                                                aTCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACAtggtgg                                       >  2:840143/1‑133 (MQ=255)                                                                                                aTCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACAtggtgg                                       <  1:840143/133‑1 (MQ=255)                                                                                                aTCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCca                                >  2:428654/1‑140 (MQ=255)                                                                                                aTCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCca                                <  2:495039/140‑1 (MQ=255)                                                                                                aTCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCca                                <  1:334373/140‑1 (MQ=255)                                                                                                aTCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCca                                <  2:625279/140‑1 (MQ=255)                                                                                                           ttCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCca                                <  2:972830/129‑1 (MQ=255)                                                                                                           ttCGAACATCCCCAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCca                                >  1:972830/1‑129 (MQ=255)                                                                                                                    ccccAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAAt            >  2:875455/1‑140 (MQ=255)                                                                                                                     cccAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATc           >  2:133913/1‑140 (MQ=255)                                                                                                                      ccAGGTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCt          >  2:290365/1‑140 (MQ=255)                                                                                                                          gTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTtcg      >  1:750961/1‑140 (MQ=255)                                                                                                                          gTTACCCGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTtcg      >  1:465855/1‑140 (MQ=255)                                                                                                                              cccGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTTCGTcgg  >  2:654271/1‑140 (MQ=255)                                                                                                                              cccGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTTCGTcgg  >  1:917057/1‑140 (MQ=255)                                                                                                                              cccGGGAAGGCCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTTCGTcgg  >  1:270100/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   CCATCGGGTTGCCAGGACCACGCGGGATCAGTTTCGAAACAAACGGCAGTGCCATTGGGTTGGCAATCGCCACTTCACGATTTGGATCAACGGAAATCCCGCCCCATTCGAACATCCCCAGGTTACCCGGGAAGACCAGCGTACCCTGTTCAGATGGCGGGGTGAAAATGCCTTCATAGCGCATCTGGTGGAACATCACGCGGCACACCAGTTGGTCAAACATGGTGGCTCCCCACATATCCGCACCGCTCAAATCTTTCGTCGG  >  NC_000913/139347‑139611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑