Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	816,176	T→C	T158A (ACT→GCT)	ybhK ←	putative transferase YbhK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	816,176	0	T	C	100.0%	193.1 / NA	55	T158A (ACT→GCT)	ybhK	putative transferase YbhK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CAGAATTGGCATCAGGCTGGTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGATTACGAATTAAATTGAT  >  NC_000913/816045‑816305                                                                                                                                    |                                                                                                                                  cagAATTGGCATCAGGCTGGTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGt                                                                                                                           <  1:186502/140‑1 (MQ=255) cagAATTGGCATCAGGCTGGTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGt                                                                                                                           <  1:545846/140‑1 (MQ=255)      ttGGCATCAGGCTGGTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGata                                                                                                                      >  2:643023/1‑140 (MQ=255)            tCAGGCTGGTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGAcc                                                                                                                <  2:171481/140‑1 (MQ=255)                    gTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTa                                                                                                        <  2:375858/140‑1 (MQ=255)                          aaaCTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTc                                                                                                  <  1:918885/140‑1 (MQ=255)                               gCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGcc                                                                                             >  2:380722/1‑140 (MQ=255)                                 cAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGcccc                                                                                           >  1:537157/1‑140 (MQ=255)                                            atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAAt                                                                                <  1:71319/140‑1 (MQ=255)                                            atgatgAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAAt                                                                                <  2:39313/140‑1 (MQ=255)                                                tgaGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGcc                                                                            <  2:537157/140‑1 (MQ=255)                                                    aTCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCa                                                                        >  1:187612/1‑140 (MQ=255)                                                    aTCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCa                                                                        >  1:291530/1‑140 (MQ=255)                                                     tCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAg                                                                       <  2:291530/140‑1 (MQ=255)                                                     tCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCCAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAg                                                                       <  1:104986/140‑1 (MQ=255)                                                     tCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTCGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAg                                                                       <  2:187612/140‑1 (MQ=255)                                                      ccGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGa                                                                      >  2:710713/1‑140 (MQ=255)                                                         cTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAAt                                                                                <  1:212499/127‑1 (MQ=255)                                                         cTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAAt                                                                                >  2:212499/1‑127 (MQ=255)                                                         cTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCa                                                                   >  1:332031/1‑140 (MQ=255)                                                         cTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCa                                                                   >  2:304645/1‑140 (MQ=255)                                                          ttCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCaa                                                                  <  1:193095/140‑1 (MQ=255)                                                           tCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAAc                                                                 <  1:380722/140‑1 (MQ=255)                                                            cATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACa                                                                >  2:829105/1‑140 (MQ=255)                                                             aTTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAg                                                               <  2:538893/140‑1 (MQ=255)                                                                  tAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGc                                                          <  1:395363/140‑1 (MQ=255)                                                                    gCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGAt                                                            <  1:130251/136‑1 (MQ=255)                                                                    gCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGAt                                                            >  2:130251/1‑136 (MQ=255)                                                                    gCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGctc                                                        >  2:812843/1‑140 (MQ=255)                                                                    gCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGctc                                                        >  2:411101/1‑140 (MQ=255)                                                                     cGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGa                                                             <  1:817346/134‑1 (MQ=255)                                                                     cGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTAAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGa                                                             >  2:817346/1‑134 (MQ=255)                                                                       tGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGa                                                     >  1:937256/1‑140 (MQ=255)                                                                         aaCCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACa                                                   >  2:760128/1‑140 (MQ=255)                                                                            cGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTg                                                >  2:485161/1‑140 (MQ=255)                                                                              ccTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGa                                              >  1:286906/1‑140 (MQ=255)                                                                               cTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGa                                                             >  2:89215/1‑124 (MQ=255)                                                                               cTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGa                                                             <  1:89215/124‑1 (MQ=255)                                                                                tCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAAt                                            >  1:203187/1‑140 (MQ=255)                                                                                 cACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATc                                           >  2:902939/1‑140 (MQ=255)                                                                                  aCGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCa                                          <  1:669495/140‑1 (MQ=255)                                                                                   cgcgTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCa                                          >  2:352053/1‑139 (MQ=255)                                                                                   cgcgTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCa                                          <  1:352053/139‑1 (MQ=255)                                                                                    gcgTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCaaa                                        <  2:937256/140‑1 (MQ=255)                                                                                        tGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCg                                    <  1:812843/140‑1 (MQ=255)                                                                                             gTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATcc                               <  2:332031/140‑1 (MQ=255)                                                                                                     ggCGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTcag                       <  1:829105/140‑1 (MQ=255)                                                                                                       cGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTcagc                      >  1:387978/1‑139 (MQ=255)                                                                                                       cGTTAACAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTcagc                      <  2:387978/139‑1 (MQ=255)                                                                                                           aaCAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGAtt                 >  2:608590/1‑140 (MQ=255)                                                                                                           aaCAATAACTCTTGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGAtt                 >  1:562965/1‑140 (MQ=255)                                                                                                                      ttGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGATTACGAATTAAAt      >  2:266003/1‑140 (MQ=255)                                                                                                                       tGAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGATTACGAATTAAAtt     >  1:50198/1‑140 (MQ=255)                                                                                                                        gAATCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGATTACGAATTAAATTg    >  1:566597/1‑140 (MQ=255)                                                                                                                          aTCGGCGTAGCTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGATTACGAATTAAATTGAt  <  2:286906/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                  CAGAATTGGCATCAGGCTGGTATAAAAACTGCCAGGCCCAATAATGATGAGATCCGCTTCATTGATAGCGTGAACCGCCTCACGCGTTGCGGGTACATTAGGCGTTAACAATAACTCTTGAATCGGCGTAGTTAACTGGTCGATATTGACCTCGCCGTAAACTTCATGCCCCTGATCGTCAATCGCCATCAGATCAACAGGATGCTCTGACATTGGAATCAAATGCGTATCCACTTTCAGCAGATTACGAATTAAATTGAT  >  NC_000913/816045‑816305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑