Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	834,209	A→G	V380V (GTA→GTG)	dinG →	ATP‑dependent DNA helicase DinG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	834,209	0	A	G	100.0%	202.2 / NA	57	V380V (GTA→GTG)	dinG	ATP‑dependent DNA helicase DinG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/32);  total (25/32)

TGCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTACGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCG  >  NC_000913/834077‑834343                                                                                                                                     |                                                                                                                                       tGCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGc                                                                                                                                 <  2:113741/140‑1 (MQ=255) tGCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGc                                                                                                                                 >  2:969586/1‑140 (MQ=255)  gCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCa                                                                                                                                <  1:318424/140‑1 (MQ=255)        ggCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAAGGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGtt                                                                                                                          >  2:881182/1‑140 (MQ=255)        ggCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGtt                                                                                                                          >  2:456439/1‑140 (MQ=255)        ggCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGtt                                                                                                                          >  2:813577/1‑140 (MQ=255)             aCTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGAttt                                                                                                                     <  1:813577/140‑1 (MQ=255)             aCTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGAttt                                                                                                                     <  2:469498/140‑1 (MQ=255)                   aGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGt                                                                                                                           >  1:36844/1‑128 (MQ=255)                   aGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGt                                                                                                                           <  2:36844/128‑1 (MQ=255)                   aGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGa                                                                                                               >  1:246674/1‑140 (MQ=255)                      tGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGa                                                                                                            >  1:397120/1‑140 (MQ=255)                         aGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAAcc                                                                                                         <  1:456439/140‑1 (MQ=255)                          gTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACcg                                                                                                        <  1:890082/140‑1 (MQ=255)                           tGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACcgc                                                                                                       >  1:323209/1‑140 (MQ=255)                            gCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACcgcg                                                                                                      <  1:649347/140‑1 (MQ=255)                            gCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACcgcg                                                                                                      <  1:969586/140‑1 (MQ=255)                                 agaTCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTg                                                                                                 <  2:397120/140‑1 (MQ=255)                                    tCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTgggg                                                                                              <  1:483075/140‑1 (MQ=255)                                     cTGCCAGTGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGa                                                                                             >  1:105876/1‑140 (MQ=255)                                     cTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGa                                                                                             >  1:137460/1‑140 (MQ=255)                                     cTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGa                                                                                             >  1:986679/1‑140 (MQ=255)                                       gCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATg                                                                                           >  1:697102/1‑140 (MQ=255)                                                                 aTGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTggcg                                                                 >  2:941475/1‑140 (MQ=255)                                                                  tgctgcGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTggcgg                                                                >  2:173018/1‑140 (MQ=255)                                                                           gCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCggctggctacg                                                       <  1:322083/140‑4 (MQ=255)                                                                           gCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCg                                                       >  1:405236/1‑140 (MQ=255)                                                                                         tttCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTc                                         <  2:373830/140‑1 (MQ=255)                                                                                         tttCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTc                                         <  2:323209/140‑1 (MQ=255)                                                                                         tttCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTc                                         <  2:986679/140‑1 (MQ=255)                                                                                         tttCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTc                                         <  1:941475/140‑1 (MQ=255)                                                                                         tttCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTc                                         <  1:630679/140‑1 (MQ=255)                                                                                          ttCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCGTCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTcc                                        >  2:405348/1‑140 (MQ=255)                                                                                             ttAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCGTCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGt                                     <  1:405348/140‑1 (MQ=255)                                                                                             ttAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGt                                     <  1:173018/140‑1 (MQ=255)                                                                                             ttAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGt                                     >  1:426341/1‑140 (MQ=255)                                                                                              tAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTg                                    >  1:971494/1‑140 (MQ=255)                                                                                              tAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTg                                    >  1:393286/1‑140 (MQ=255)                                                                                                aCGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCa                                  <  2:65358/140‑1 (MQ=255)                                                                                                aCGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTACa                                  >  2:791176/1‑140 (MQ=255)                                                                                                   aTTTAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGACGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCg                               <  1:41823/140‑1 (MQ=255)                                                                                                     ttAAGTGAGAAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGTGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGt                             <  2:246674/140‑1 (MQ=255)                                                                                                            agaAAACCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCaaa                      >  1:598594/1‑140 (MQ=255)                                                                                                              aaaaCCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATg                    >  1:44818/1‑140 (MQ=255)                                                                                                              aaaaCCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATg                    >  1:757176/1‑140 (MQ=255)                                                                                                              aaaaCCGGCAGCCATGACATTGTGCGTCTGCATCGGTAGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAGTCTTCCGGTGCACCGGTGACTAAATg                    >  2:871225/1‑140 (MQ=255)                                                                                                                 aCCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGc                 <  1:881182/140‑1 (MQ=255)                                                                                                                 aCCGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTACACCGGTGACCAAATGGGc                 <  1:791176/140‑1 (MQ=255)                                                                                                                   cGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGa               <  2:44818/140‑1 (MQ=255)                                                                                                                   cGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGa               <  2:610702/140‑1 (MQ=255)                                                                                                                   cGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGa               <  2:697102/140‑1 (MQ=255)                                                                                                                   cGGCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGa               <  2:105876/140‑1 (MQ=255)                                                                                                                     gCAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGAcg             <  2:426341/140‑1 (MQ=255)                                                                                                                      cAGCCATGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGAcgc            >  1:322401/1‑140 (MQ=255)                                                                                                                         ccATGACATTGTGCATCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCggg         <  2:647499/140‑1 (MQ=255)                                                                                                                           aTGACATTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGaa       <  1:40350/140‑1 (MQ=255)                                                                                                                                aTTGTGCGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAgcg  <  2:757176/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TGCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATTGTACGTCTGCATCGGTTGATTTTGCAGATGAACCGCGCGTTGGGGATGTTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCG  >  NC_000913/834077‑834343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑