Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,923	A→G	intergenic (‑104/‑166)	ybjL ← / → ybjM	putative transport protein YbjL/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,923	0	A	G	100.0%	191.6 / NA	54	intergenic (‑104/‑166)	ybjL/ybjM	putative transport protein YbjL/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/34);  total (20/34)

CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAATG  >  NC_000913/889788‑890057                                                                                                                                        |                                                                                                                                       ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                    <  2:185631/140‑1 (MQ=255)       aaCAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaa                                                                                                                              >  2:568524/1‑140 (MQ=255)       aaCAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaa                                                                                                                              >  1:869337/1‑140 (MQ=255)           aTTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagtaa                                                                                                                          >  1:172557/1‑140 (MQ=255)           aTTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagtaa                                                                                                                          <  1:786917/140‑1 (MQ=255)            ttCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAAc                                                                                                                         >  2:575005/1‑140 (MQ=255)             tCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACa                                                                                                                        >  1:310830/1‑140 (MQ=255)             tCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACa                                                                                                                        <  1:568524/140‑1 (MQ=255)              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                       <  1:280963/140‑1 (MQ=255)              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                       <  1:575005/140‑1 (MQ=255)              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                       <  1:35176/140‑1 (MQ=255)                       ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                              <  1:571049/140‑1 (MQ=255)                       ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGA‑AATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                             <  1:162393/140‑1 (MQ=255)                        tataTTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCACAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                             <  1:215606/140‑1 (MQ=255)                         atatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACt                                                                                                            <  2:639452/140‑1 (MQ=255)                            ttCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAgt                                                                                                         <  2:172557/140‑1 (MQ=255)                                cGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                     <  1:973725/140‑1 (MQ=255)                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                    <  1:633589/140‑1 (MQ=255)                                     gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATcaca                                                                                                <  1:808763/140‑1 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                             <  2:77303/126‑1 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                             >  1:77303/1‑126 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                     <  2:831151/134‑1 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                     >  1:831151/1‑134 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                    <  2:789213/135‑1 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                    >  1:789213/1‑135 (MQ=255)                                         aCTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAg                                                                                            <  1:187390/140‑1 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATca                                                                                                  >  1:549353/1‑128 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATca                                                                                                  <  2:818859/128‑1 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATca                                                                                                  <  2:549353/128‑1 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATca                                                                                                  >  1:818859/1‑128 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                      <  2:972894/140‑1 (MQ=255)                                                gTTTACTAGTAAGCTGTTGAAAGAAATGGTAACTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCaa                                                                                     <  2:869337/140‑1 (MQ=255)                                                   tACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACgg                                                                                  >  1:295681/1‑140 (MQ=255)                                                     cTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCt                                                                                >  1:689251/1‑140 (MQ=255)                                                           aGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGa                                                                          >  1:323798/1‑140 (MQ=255)                                                           aGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCACTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGa                                                                          >  2:736742/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                     >  2:872697/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                     >  2:513727/1‑140 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                   <  2:121455/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                   <  1:337739/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                   <  2:23285/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                   <  1:82513/140‑1 (MQ=255)                                                                     agaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                   >  1:166467/1‑137 (MQ=255)                                                                     agaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                   <  2:166467/137‑1 (MQ=255)                                                                      gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                               <  2:689251/140‑1 (MQ=255)                                                                      gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                               <  2:295681/140‑1 (MQ=255)                                                                       aaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                              <  2:310830/140‑1 (MQ=255)                                                                                      aTAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGaa                                               >  2:566096/1‑140 (MQ=255)                                                                                        aaTGTTTTTTACCAGAATTCAGGGCGCAGATTCACTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATg                                             <  1:736742/140‑1 (MQ=255)                                                                                           gTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGa                                          <  2:139026/140‑1 (MQ=255)                                                                                               tttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCg                                      >  1:159884/1‑140 (MQ=255)                                                                                                     aGAATTCAGGGCGCAGATTCAGTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTa                                <  2:895576/140‑1 (MQ=255)                                                                                                            aGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACt                         <  1:566096/140‑1 (MQ=255)                                                                                                                                   ccTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAAtg  >  1:87691/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAATG  >  NC_000913/889788‑890057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑