Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	925,047	A→G	D595G (GAC→GGC)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,047	0	A	G	100.0%	173.7 / NA	50	D595G (GAC→GGC)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

GGTCATCAAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGACTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGG  >  NC_000913/924919‑925178                                                                                                                                 |                                                                                                                                    ggTCATCAAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACg                                                                                                                          <  1:314134/140‑1 (MQ=255) ggTCATCAAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACg                                                                                                                          <  1:465633/140‑1 (MQ=255)     atcaAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACgtggt                                                                                                                      >  2:134710/1‑140 (MQ=255)     atcaAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACgtggt                                                                                                                      >  2:182241/1‑140 (MQ=255)      tcaAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACgtggtg                                                                                                                     <  1:313192/140‑1 (MQ=255)       caAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGc                                                                                                                    <  2:953694/140‑1 (MQ=255)             tCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGtga                                                                                                              >  2:122107/1‑140 (MQ=255)             tCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGtga                                                                                                              >  1:345042/1‑140 (MQ=255)               cacaTGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGtgatg                                                                                                            >  1:877075/1‑140 (MQ=255)               cacaTGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGtgatg                                                                                                            <  2:28601/140‑1 (MQ=255)                    gCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACgtggtg                                                                                                                     <  2:565625/126‑1 (MQ=255)                    gCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACgtggtg                                                                                                                     >  1:565625/1‑126 (MQ=255)                       gTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTg                                                                                                                  <  2:287015/126‑1 (MQ=255)                       gTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTg                                                                                                                  >  1:287015/1‑126 (MQ=255)                         gctgctgctGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACg                                                                                                  >  1:500164/1‑140 (MQ=255)                         gctgctgctGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACg                                                                                                  >  2:841268/1‑140 (MQ=255)                          ctgctgctgGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGc                                                                                                 <  1:281132/140‑1 (MQ=255)                               gctgGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCgggg                                                                                            >  2:949902/1‑140 (MQ=255)                                ctgGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGt                                                                                           <  2:582961/140‑1 (MQ=255)                                ctgGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGt                                                                                           <  2:377583/140‑1 (MQ=255)                                        aaTCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGaaa                                                                                   >  2:968272/1‑140 (MQ=255)                                           cGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTg                                                                                <  2:619708/140‑1 (MQ=255)                                                        ccGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCAt                                                                   <  2:769352/140‑1 (MQ=255)                                                            aCGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGt                                                               >  1:756681/1‑140 (MQ=255)                                                                gtTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTa                                                           >  1:429105/1‑140 (MQ=255)                                                                   cAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATcc                                                        <  1:943635/140‑1 (MQ=255)                                                                    aaTATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATcca                                                       <  1:841268/140‑1 (MQ=255)                                                                     atatTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATccac                                                      <  1:182241/140‑1 (MQ=255)                                                                        ttCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg                                                   <  2:433319/140‑1 (MQ=255)                                                                          cTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGa                                                 <  1:246926/140‑1 (MQ=255)                                                                           tGTTGCAGGTGATGGATAACGGTACGCTGACCAATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGAt                                                >  1:81018/1‑140 (MQ=255)                                                                                cAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAg                                           >  2:214780/1‑140 (MQ=255)                                                                                   gTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCAc                                        <  1:593035/140‑1 (MQ=255)                                                                                       tGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg                                                   <  1:916801/125‑1 (MQ=255)                                                                                       tGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAg                                                   >  2:916801/1‑125 (MQ=255)                                                                                       tGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcgat                                    >  1:594176/1‑140 (MQ=255)                                                                                       tGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcgat                                    >  1:594857/1‑140 (MQ=255)                                                                                          aTAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcgatgcg                                 <  2:791825/140‑1 (MQ=255)                                                                                           tAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcgatgcga                                <  2:594176/140‑1 (MQ=255)                                                                                             aCGGTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACcgatgcgatg                              >  2:642972/1‑140 (MQ=255)                                                                                                gTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGaa                           <  2:259691/140‑1 (MQ=255)                                                                                                gTACGCTGACCGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGaa                           >  1:259691/1‑140 (MQ=255)                                                                                                         ccGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCaag                  >  1:669568/1‑140 (MQ=255)                                                                                                         ccGATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCaag                  <  1:134710/140‑1 (MQ=255)                                                                                                           gATAACAACGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCaagaa                >  2:675245/1‑140 (MQ=255)                                                                                                                  acGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCaagaa                >  2:413197/1‑133 (MQ=255)                                                                                                                  acGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCaagaa                <  1:413197/133‑1 (MQ=255)                                                                                                                  acGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCttt         >  2:160554/1‑140 (MQ=255)                                                                                                                  acGGACGCAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCttt         >  1:95203/1‑140 (MQ=255)                                                                                                                         cAAAGCAGGCTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCgg  <  2:500164/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    GGTCATCAAGCATCCACATGCGGTGCTGCTGCTGGACGAAATCGAGAAAGCGCACCCGGACGTGTTCAATATTCTGTTGCAGGTGATGGATAACGGTACGCTGACCGATAACAACGGACGCAAAGCAGACTTCCGTAACGTGGTGCTGGTGATGACCACCAACGCCGGGGTACGGGAAACTGAGCGCAAATCCATTGGTCTTATCCACCAGGATAACAGCACCGATGCGATGGAAGAGATCAAGAAGATCTTTACACCGG  >  NC_000913/924919‑925178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑