Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	955,039	A→G	Y532H (TAC→CAC)	ycaO ←	ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	955,039	0	A	G	100.0%	204.7 / NA	59	Y532H (TAC→CAC)	ycaO	ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/34);  total (25/34)

CTGCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTACATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATC  >  NC_000913/954902‑955169                                                                                                                                          |                                                                                                                                   cTGCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCa                                                                                                                                  >  1:907517/1‑140 (MQ=255) cTGCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCa                                                                                                                                  >  1:791550/1‑140 (MQ=255) cTGCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCa                                                                                                                                  >  1:485365/1‑140 (MQ=255) cTGCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCa                                                                                                                                  >  1:409767/1‑140 (MQ=255)   gCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATg                                                                                                                                <  2:374290/140‑1 (MQ=255)     aGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCg                                                                                                                              <  2:791550/140‑1 (MQ=255)           tCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACaaa                                                                                                                        <  1:643496/140‑1 (MQ=255)           tCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACaaa                                                                                                                        <  2:475339/140‑1 (MQ=255)           tCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACaaa                                                                                                                        <  2:651910/140‑1 (MQ=255)            cGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAAc                                                                                                                       >  1:12812/1‑140 (MQ=255)                            cGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTc                                                                                                                 >  2:850120/1‑130 (MQ=255)                            cGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTc                                                                                                                 <  1:850120/130‑1 (MQ=255)                                 gATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGc                                                                                                  <  2:966723/140‑1 (MQ=255)                                 gATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGc                                                                                                  >  1:66434/1‑140 (MQ=255)                                   tgtgCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTg                                                                                                >  2:133519/1‑140 (MQ=255)                                     tgCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGc                                                                                              <  1:45080/140‑1 (MQ=255)                                     tgCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGc                                                                                              <  2:661724/140‑1 (MQ=255)                                     tgCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGc                                                                                              <  2:563954/140‑1 (MQ=255)                                      gcagcaAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTAGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCg                                                                                             >  2:725344/1‑140 (MQ=255)                                           aaaCGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGAtctt                                                                                        <  1:726376/140‑1 (MQ=255)                                            aaCGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGAtcttc                                                                                       >  2:155035/1‑140 (MQ=255)                                             aCGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGc                                                                                              >  1:127357/1‑132 (MQ=255)                                             aCGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGc                                                                                              <  2:127357/132‑1 (MQ=255)                                                 gTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCt                                                                                  <  1:259684/140‑1 (MQ=255)                                                    cAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCtgtg                                                                               <  1:52551/140‑1 (MQ=255)                                                    cAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCtgtg                                                                               <  2:12812/140‑1 (MQ=255)                                                    cAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCtgtg                                                                               <  2:857721/140‑1 (MQ=255)                                                    cAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCtgtg                                                                               <  2:409767/140‑1 (MQ=255)                                                      gATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGcc                                                                             <  1:735758/140‑1 (MQ=255)                                                      gATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGcc                                                                             >  2:548837/1‑140 (MQ=255)                                                           cTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGtaa                                                                        >  1:344623/1‑140 (MQ=255)                                                                 aCCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATaacaa                                                                  >  1:611777/1‑140 (MQ=255)                                                                       tGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTg                                                            <  2:611777/140‑1 (MQ=255)                                                                        gCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTgc                                                           >  1:30357/1‑140 (MQ=255)                                                                          aGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTgcag                                                         >  1:982828/1‑140 (MQ=255)                                                                            gCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTgcaggc                                                       <  2:907517/140‑1 (MQ=255)                                                                                  aaaCGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGat                                                 <  2:930797/140‑1 (MQ=255)                                                                                        cgcTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGt                                           >  1:959176/1‑140 (MQ=255)                                                                                          cTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTg                                         >  1:126708/1‑140 (MQ=255)                                                                                          cTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTg                                         >  1:766261/1‑140 (MQ=255)                                                                                            tCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGgc                                       <  2:30357/140‑1 (MQ=255)                                                                                                      ttGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGa                             <  1:13442/140‑1 (MQ=255)                                                                                                      ttGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGa                             <  1:733208/140‑1 (MQ=255)                                                                                                       tGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGAc                            >  2:363614/1‑140 (MQ=255)                                                                                                       tGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGAc                            >  1:419267/1‑140 (MQ=255)                                                                                                        gCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACt                           <  1:272679/140‑1 (MQ=255)                                                                                                        gCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACt                           <  2:766261/140‑1 (MQ=255)                                                                                                        gCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACt                           <  1:363614/140‑1 (MQ=255)                                                                                                                ggcggcTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACtg                   <  1:562078/140‑1 (MQ=255)                                                                                                                agcggcTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACtg                   <  1:725344/139‑1 (MQ=255)                                                                                                                 gcggcTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTcc                                <  2:310360/126‑1 (MQ=255)                                                                                                                 gcggcTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTcc                                >  1:310360/1‑126 (MQ=255)                                                                                                                   ggcTTCTACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACtgatg                <  2:982828/140‑1 (MQ=255)                                                                                                                         tACGGCATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTaa          <  1:133519/140‑1 (MQ=255)                                                                                                                              cATCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTcc     <  2:419267/140‑1 (MQ=255)                                                                                                                                tCTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCAt   >  2:881728/1‑140 (MQ=255)                                                                                                                                 cTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCa    >  1:637039/1‑138 (MQ=255)                                                                                                                                 cTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCa    <  2:637039/138‑1 (MQ=255)                                                                                                                                 cTGCGCCGTGCATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATc  >  2:923149/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   CTGCAGCTTTTCGTAGGCCTTCAACAACGACTGATGTGCAGCAAACGCGTGCAGATCGCTATCTACCGGTTGCAGGCCGTAAAACGCCGCTTCGCCGCTCATTGCCGCACTGGCGGCTTCTACGGCATCTGCGCCGTACATGCGAACAAACGCATTCAGATATTGCAGCGGCTGGCGATCTTCTTCCTGTGCCAGTAATAACAACGTTTGCAGGCAGCGATAATAGTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATC  >  NC_000913/954902‑955169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑