Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,004,562	A→G	S206G (AGC→GGC)	ycbF →	putative fimbrial chaperone YcbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,004,562	0	A	G	100.0%	157.6 / NA	45	S206G (AGC→GGC)	ycbF	putative fimbrial chaperone YcbF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

CGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGT  >  NC_000913/1004427‑1004697                                                                                                                                        |                                                                                                                                         cGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGc                                                                                                                                        >  1:202939/1‑138 (MQ=255)               ttCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCg                                                                                                                        <  2:535564/140‑1 (MQ=255)                 cGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  2:902527/140‑1 (MQ=255)                 cGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  2:822860/140‑1 (MQ=255)                 cGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  1:623585/140‑1 (MQ=255)                       gtgtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  2:983453/134‑1 (MQ=255)                       gtgtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      >  1:983453/1‑134 (MQ=255)                         gtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCg                                                                                                                   >  2:545194/1‑135 (MQ=255)                         gtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCg                                                                                                                   <  1:545194/135‑1 (MQ=255)                          tACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCAt                                                                                                             <  1:436314/140‑1 (MQ=255)                                tCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAACCGCACCGTGTCAt                                                                                                             >  1:378700/1‑134 (MQ=255)                                tCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAACCGCACCGTGTCAt                                                                                                             <  2:378700/134‑1 (MQ=255)                                  aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTg                                                                                                     >  1:341041/1‑140 (MQ=255)                                  aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTg                                                                                                     >  2:471762/1‑140 (MQ=255)                                       cccAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTggcgtg                                                                                                >  1:397954/1‑138 (MQ=255)                                       cccAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTggcggg                                                                                                >  1:28496/1‑140 (MQ=255)                                           aCGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGAt                                                                                            >  2:115639/1‑140 (MQ=255)                                            cGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATa                                                                                           <  2:461454/140‑1 (MQ=255)                                                  attaCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGAt                                                                                     >  2:378605/1‑140 (MQ=255)                                                  attaCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGAt                                                                                     >  1:768903/1‑140 (MQ=255)                                                         tAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTAcggcg                                                                              >  1:279285/1‑140 (MQ=255)                                                           aCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCggg                                                                            >  1:711236/1‑140 (MQ=255)                                                             cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          <  1:115639/140‑1 (MQ=255)                                                             cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          >  2:451329/1‑140 (MQ=255)                                                             cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          <  2:279285/140‑1 (MQ=255)                                                             cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          <  2:188309/140‑1 (MQ=255)                                                                aTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAg                                                                       >  2:539133/1‑140 (MQ=255)                                                                     ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTAcg                                                                                 <  1:134252/125‑1 (MQ=255)                                                                     ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTAcg                                                                                 >  2:134252/1‑125 (MQ=255)                                                                     ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGCaa                                                                  >  1:910462/1‑140 (MQ=255)                                                                                   gTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGGGGGAAGAAGGA‑GGaaaaa                                                   >  2:647107/1‑139 (MQ=255)                                                                                    tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATc                                                  <  1:554241/140‑1 (MQ=255)                                                                                    tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATc                                                  <  1:554369/140‑1 (MQ=255)                                                                                    tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATc                                                  <  1:471762/140‑1 (MQ=255)                                                                                     aaaGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGGAAAAAAGA‑GCgaaaaaa                                                 >  2:11622/1‑137 (MQ=255)                                                                                       aGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGc                                               <  1:464040/140‑1 (MQ=255)                                                                                           ttAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGGAAAAAAGA‑GGAAAAACAGccccc                                           >  2:507536/1‑138 (MQ=255)                                                                                              aGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGaaa                                        <  1:118797/140‑1 (MQ=255)                                                                                                   tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGTGGAAAGAAAGAGGCAAAAACTCCCCGTAAAAAgg                                    >  1:378019/1‑140 (MQ=255)                                                                                                       ggAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGTGGCAAGAAAGA‑GCAAAAACATCCCTCAAAAAAGGTCgg                               >  1:228552/1‑140 (MQ=255)                                                                                                                          gCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGttttttt            <  1:799883/140‑1 (MQ=255)                                                                                                                               cGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCg       <  1:647107/140‑1 (MQ=255)                                                                                                                               cGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCg       <  1:539133/140‑1 (MQ=255)                                                                                                                                    gACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGt  <  1:553089/140‑1 (MQ=255)                                                                                                                                    gACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGt  <  1:11622/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGT  >  NC_000913/1004427‑1004697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑