Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,248,432	A→G	L228P (CTG→CCG)	dhaM ←	dihydroxyacetone kinase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,248,432	0	A	G	100.0%	148.5 / NA	43	L228P (CTG→CCG)	dhaM	dihydroxyacetone kinase subunit M
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/24);  total (19/24)

TTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATC  >  NC_000913/1248297‑1248559                                                                                                                                        |                                                                                                                                tttGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGc                                                                                                                             <  1:368147/140‑1 (MQ=255)      cTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTCCCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCtt                                                                                                                        <  1:951153/140‑1 (MQ=255)              tACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCt                                                                                                                >  2:88462/1‑140 (MQ=255)              tACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCt                                                                                                                >  2:139809/1‑140 (MQ=255)              tACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCt                                                                                                                >  2:827007/1‑140 (MQ=255)                        tGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCg                                                                                                      <  2:108421/140‑1 (MQ=255)                        tGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCg                                                                                                      <  1:319770/140‑1 (MQ=255)                          gTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGTAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCa                                                                                                    >  1:324649/1‑140 (MQ=255)                          gTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGcc                                                                                                            >  1:412453/1‑132 (MQ=255)                          gTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGcc                                                                                                            <  2:412453/132‑1 (MQ=255)                           ttGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCaa                                                                                                   >  2:652781/1‑140 (MQ=255)                                  taaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGgcg                                                                                            <  1:652781/140‑1 (MQ=255)                                  taaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGgcg                                                                                            <  2:722370/140‑1 (MQ=255)                                  taaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGgcg                                                                                            <  1:934288/140‑1 (MQ=255)                                    aaaGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCa                                                                                          >  1:326936/1‑140 (MQ=255)                                          tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt                                                                                    >  1:563937/1‑140 (MQ=255)                                            tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCg                                                                                       >  1:36314/1‑135 (MQ=255)                                            tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCg                                                                                       <  2:36314/135‑1 (MQ=255)                                            tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCg                                                                                  <  1:563423/140‑1 (MQ=255)                                            tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAACCAGGCGCAGCg                                                                                       >  2:99460/1‑135 (MQ=255)                                            tACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAACCAGGCGCAGCg                                                                                       <  1:99460/135‑1 (MQ=255)                                             aCCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGt                                                                                 >  1:736123/1‑140 (MQ=255)                                                   aaCAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGc                                                                           <  2:563937/140‑1 (MQ=255)                                                        gCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACt                                                                      >  1:663317/1‑140 (MQ=255)                                                          ggAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTg                                                                    <  1:88462/140‑1 (MQ=255)                                                                  aCGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagta                                                               >  1:738767/1‑137 (MQ=255)                                                                  aCGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagta                                                               <  2:738767/137‑1 (MQ=255)                                                                      agagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt                                                        <  2:518093/140‑1 (MQ=255)                                                                      agagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt                                                        <  2:5427/140‑1 (MQ=255)                                                                      agagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt                                                        <  1:240159/140‑1 (MQ=255)                                                                        agTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCt                                                      <  1:139809/140‑1 (MQ=255)                                                                               ggAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAAt                                               <  2:154749/140‑1 (MQ=255)                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAct                                            >  2:215114/1‑140 (MQ=255)                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAct                                            >  2:917756/1‑140 (MQ=255)                                                                                       ttCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTg                                       <  2:140273/140‑1 (MQ=255)                                                                                            ccGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGtgtg                                  >  1:716755/1‑140 (MQ=255)                                                                                                ttCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGc                              >  2:316509/1‑140 (MQ=255)                                                                                                 tCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCa                             >  1:459698/1‑140 (MQ=255)                                                                                                              cTTCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGTCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGtttttt                <  2:736123/140‑1 (MQ=255)                                                                                                                tCAGCCAGCTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTc              <  2:716755/140‑1 (MQ=255)                                                                                                                   gccagcTGACGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAg           <  2:663317/140‑1 (MQ=255)                                                                                                                           aCGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCTAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAatatat   <  2:684188/140‑1 (MQ=255)                                                                                                                            cGGAAAGCGATCGGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATc  >  2:209122/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                TTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATC  >  NC_000913/1248297‑1248559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑